Expression and clinical significance of miR-122 and miR-29 in hepatitis B virus-related liver disease

Abstract

ABSTRACT. MicroRNA molecules have been increasingly regarded as a diagnostic and prognostic marker of certain diseases. The aim of this study was to investigate the expression and clinical significance of miR-122 and miR-29 in liver disease related to hepatitis B virus infection. The serum levels of miR-122 and miR-29 in 20 patients with hepatocellular carcinoma (HCC), 20 patients with liver cirrhosis (LC), 29 patients with chronic hepatitis B (CHB), 20 cases of hepatitis B virus carriers (ASC), and 20 healthy controls (HC) were determined by a fluorescence real-time quantitative PCR method and then evaluated by clinical correlation analysis. Compared with the serum levels of miR-122 in the HC, LC, and ASC groups, those in patients with HCC and CHB were significantly increased. The serum levels of miR-29 in LC patients were lower than those in the healthy controls (P < 0.01). A positive correlation was observed between the expression of miR-122 and miR-29, and HBV DNA in patients with CHB. A negative correlation was found between miR-29 and α-fetoprotein in patients with HCC. The elevation in miR-122 was correlated with liver damage in CHB patients and with the pathogenesis of liver cancer in HCC patients. The decrease in miR-29 expression was related to the incidence

Non-coding RNA-mediated epigenetic regulation of liver fibrosis

“…13 associated with chronic HBV infection [106]. In addition, miR-29b was involved in the hypomethylation of PTEN by curcumin.…”

Circulating miR-122 levels in self-recovering hepatitis E patients

“…These miRNAs exhibit a consistent expression profile among healthy individuals. miR-122, the most abundant hepatic microRNA, has been evaluated mainly in chronic hepatitis B virus (HBV), hepatitis C virus (HCV) infections and hepatocellular carcinoma (HCC) [17][18][19][20][21][22][23][24].…”

Correlation between circulating miR‐122 and prognosis of chronic HBV‐related liver failure

“…Roderburg et al analyzed serum miR‐122 expression in 223 patients with critical illness and 57 with cirrhosis, demonstrating that an elevated serum miR‐122 level was an independent marker of ongoing liver injury regardless of the underlying disease. As miR‐122 is correlated with liver damage in chronic hepatitis B (CHB) patients, it can be employed as a potential biomarker for the evaluation of CHB . In vitro studies have shown that miR‐122 could inhibit HBV replication .…”

“…As miR-122 is correlated with liver damage in chronic hepatitis B (CHB) patients, 17 it can be employed as a potential biomarker for the evaluation of CHB. 17,18 In vitro studies have shown that miR-122 could inhibit HBV replication. 19 In China, CHB-related liver failure (CHBLF) has extremely high morbidity and mortality.…”