Expression and clinical significance of microRNA‑21, PTEN and p27 in cancer tissues of patients with non‑small cell lung cancer

Abstract

Expression and clinical significance of micro-RNA-21, PTEN and p27 in cancer tissue of patients with non-small cell lung cancer (NSCLC) were investigated. In this study, cancer tissue and adjacent tissue specimens from 230 patients with NSCLC were collected from thoracic surgery department in Hubei Cancer Hospital from March 2010 to February 2016. The expression of miRNA-21, PTEN and p27 in cancer tissue and adjacent tissue of patients with NSCLC was detected by RT-PCR. Combined with clinical information, the correlation among miRNA-21, PTEN, p27 and clinical features of NSCLC was analyzed. The expression of miRNA-21, PTEN, p27 in NSCLC was significantly lower than that in adjacent tissue by RT-PCR (P<0.05). There was no significant difference in age, sex and course of disease (P>0.050), but there were differences in smoking, lymph node metastasis, TNM stage and differentiation degree classification (P<0.050). By comparing the 3-year survival rate in the group with high and low expression of miRNA-21, PTEN and p27, it was found that the 36-month survival rate of patients with high expression of miRNA-21 was 85.19% (P<0.05), and of patients with low expression of miRNA-21 it was 95.90% (P<0.05). The 36-month survival rate of patients with high expression of PTEN was 85.59% (P<0.05), of patients with low expression of PTEN it was 94.96% (P<0.05) and in patients with high expression of p27 it was 84.91% (P<0.05). The 36-month survival rate of patients with low expression of p27 was 94.35% (P<0.05). The survival rates of miRNA-21, PTEN and p27 low expression groups were significantly higher than those of high expression groups (P<0.05). In conclusion, the expression of miRNA-21, PTEN and p271 in cancer tissue of NSCLC patients was low. The three indexes have good diagnostic efficacy based on ROC curve analysis, and are expected to be excellent indexes for early clinical diagnosis and prognosis of NSCLC.

MicroRNA-21 as a diagnostic and prognostic biomarker of lung cancer: a systematic review and meta-analysis

“…A total of 1603 participants from 15 studies [ 22 , 50–72 ] were included in our meta-analysis of prognosis. Among these studies, 12 provided data on OS of lung cancer patients [ 60 , 62–72 ], 1 on PFS [ 61 ], 1 on DFS [ 22 ].…”

Effect of Statins on Lung Cancer Molecular Pathways: A Possible Therapeutic Role

“…However, the cohort was too small to make definitive statements [ 101 ]. Similarly, there was a certain correlation between smoking, TNM staging, lymph node metastasis, differentiation and miRNA-21, PTEN and p27 expression [ 46 ].…”

“…Protein p27 expression seems to be associated with a better prognosis [ 45 ]. In a systematic review, Zhuang et al [ 46 ] reported that in NSCLC patients, a high p27 expression was associated with better survival. This data may be interesting if integrated with those reported in the previous section.…”

Clinicopathological significance and prognostic value of P27 expression in gastric cancer patients: a meta-analysis

“…A previous study proved that low P 27 expression developed metastasis and predicted a poorer prognosis of eyelid sebaceous gland carcinoma [11]. To our knowledge, low P 27 levels have also been associated with poor survival outcomes in other types of cancers, such as ovarian cancer [12], breast cancer [13], papillary thyroid carcinoma [14], non-small cell lung cancer [15] and hepatocellular carcinoma [16]. Recent studies demonstrated that P 27 represents lower expression in gastric cancer patients compared to healthy volunteers [4,10,14].…”