Expression and Clinical Significance of miRNA-34a in Colorectal Cancer

Ma, Zongwen; Kong, Xiaolin; Cui, Gang; Ren, Cuicui; Zhang, Yingjie; Fan, Shengjin; Li, Yinghua

doi:10.7314/apjcp.2014.15.21.9265

Cited by 12 publications

(9 citation statements)

References 33 publications

(28 reference statements)

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“…This accordance with the study of Ma et al who found that Polyps tissues had significantly higher miRNA34a gene expression than para-neoplastic and colorectal cancer tissue samples [17] .…”

Section: Discussionsupporting

confidence: 92%

The clinical impact of miRNA34a and P53 gene expression in colon cancer

Badr

Assar

Gohar

et al. 2018

Biochemistry and Biophysics Reports

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ObjectiveTo study the potential role of miRNA34a gene expression and its relationship with P53 gene expression, fate, stage, metastasis and overall survival of colorectal cancer.Patients and methodsThis study was carried out 30 patients with colon adenocarcinoma, 30 patients with benign colon polyp and 30 apparently healthy persons served as controls. All participants were subjected to full history taking, general clinical examination. Complete blood count, liver and kidney function, determination of serum tumor markers were done. Estimation of microRNA 34a and P53 Gene expression by real-time PCR were done.ResultsThere was a significant negative relationship between serum tumor markers and micro RNA 34a gene expression in cancer patients. Also, there was a statistically significant positive relationship between miRNA34a gene expression and P53 gene expression in both patients groups. The diagnostic accuracy of miRNA34a gene expression was both sensitive and specific for colon cancer. MiRNA34a and P53 gene expression had statistically significant relation with tumor stage and presence of metastases.ConclusionIt can be concluded that the level of miRNA34a can be used to differentiate between colon cancers and begin adenomas. MiRNA34a can be used as a prognostic marker in colon cancer.

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“…This accordance with the study of Ma et al who found that Polyps tissues had significantly higher miRNA34a gene expression than para-neoplastic and colorectal cancer tissue samples [17] .…”

Section: Discussionsupporting

confidence: 92%

The clinical impact of miRNA34a and P53 gene expression in colon cancer

Badr

Assar

Gohar

et al. 2018

Biochemistry and Biophysics Reports

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“…miR-34a-5p expression has been shown to be directly regulates the expression of proteins involved in cell cycle, differentiation, apoptosis, and antagonizes processes, including cervical, ovarian and testicular cancer (19,20). Ma et al conducted a series of studies on miR-34a-5p, and were the first to confirm its pro-apoptotic function (21). Moreover, miR-34a-5p has been found to inhibit cell proliferation and invasion in vitro, which suggested that miR-34a-5p might play a role in inhibiting tumor recurrence (22).…”

Section: Discussionmentioning

confidence: 99%

miRNA-34a-5p downregulation of VEGFA in endometrial stem cells contributes to the pathogenesis of endometriosis

Huang

Chen

2017

Molecular Medicine Reports

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Endometrial-derived stem cells (EnSCs) serve an important role in the development of endometriosis via retrograde menstruation. Abnormal expression of miRNAs in EnSCs is involved in the etiology of endometriosis, however, the mechanisms remain unclear. The aim of the present study was to investigate the expression of miR‑34a‑5p and VEGFA in endometrial samples from patients with or without endometriosis, and then examine the underlying mechanism of microRNA‑34a‑5p regulation of VEGFA in EnSCs. Endometrial samples from patients with or without endometriosis were collected, and miR‑34a‑5p expression in the two groups was measured using RT‑PCR. Human endometrial‑derived stem cells (hEnSCs) were isolated from these endometrial samples, and hEnSCs were transfected with the miR‑34a‑5p mimics or control miRNAs. qPCR and western blotting were performed to assess the effects of miR‑34a‑5p on the expression of VEGFA in hEnSCs, and cell growth was assessed by an MTT assay. miR‑34a‑5p was significantly downregulated in patients with endometriosis when compared with that of those without endometriosis. VEGFA expression levels in hEnSCs with an overexpression of miR‑34a‑5p were significantly reduced when compared with those in the negative control (P<0.01). In addition, the upregulation of miR‑34a‑5p suppressed EnSCs proliferation by targeting the 3' untranslated region of VEGFA. miR‑34a‑5p provides a novel avenue for the understanding of the development of endometriosis, and may facilitate the development of potential therapeutics against endometriosis.

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“…Through binding target mRNA molecules, miRNAs generally downregulate gene expression and can influence cell proliferation, apoptosis, and the cell cycle 7–9. Dysregulated miRNA expression is a common feature in human cancers,10,11 and previous studies have demonstrated that several miRNAs are dysregulated in colorectal cancer (CRC), and associated with its progression 12–14. Aberrant expression of miRNAs is increasingly recognized as involved in the inappropriate stimulation of cellular programs, such as autophagy 15,16…”

Section: Introductionmentioning

confidence: 99%

<p>Overexpression of microRNA-216a inhibits autophagy by targeting regulated MAP1S in colorectal cancer</p>

Wang¹,

Zhang²,

Dang³

et al. 2019

OTT

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Background: Autophagy executes the rapid degradation of unneeded proteins and organelles through the lysosomal pathway, and is a crucial catabolic process widely conserved among eukaryotes. miRNAs can modulate autophagy by targeting genes encoding proteins involved in the process. A great deal of researchhas indicated that miR-216a was a functional miRNA related to tumorigenesis. However, the contribution of miR-216a to autophagy in colorectal cancer (CRC) remains unclear. The purpose of this study was to investigate the role of miR-216a in autophagy in CRC cells. Methods: The expression levels of miR-216a in 67 paired CRC patients were evaluated by qRT-PCR. Direct gene targeting predicted by TargetScan and miRanda was confirmed by luciferase activity. Western blot and flow cytometry were used to identify the regulatory mechanism of miR-216a on autophagy in CRC cells. Results: We determined that miR-216a is downregulated in CRC by screening its expression in 67 CRC tissue samples. Dual luciferase reporter assays showed that miR-216a binds the 3′-UTR of MAP1S, suggesting that MAP1S is a direct target of miR-216a. miR-216a could inhibit autophagy in HCT-116 and HT-29 CRC cells through downregulating MAP1S expression. Flow cytometry and Western blot analysis demonstrated that overexpression of miR-216a reduced MAP1S mRNA and protein levels. Moreover, we determined that miR-216a-regulated inhibition of autophagy via MAP1S regulation involves the TGF-β pathway. Conclusion: Taken together, our findings indicate that miR-216a was a tumor-suppressor miRNA in human CRC, which can inhibit autophagy via the TGF-β/MAP1S pathway.

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Expression and Clinical Significance of miRNA-34a in Colorectal Cancer

Cited by 12 publications

References 33 publications

The clinical impact of miRNA34a and P53 gene expression in colon cancer

The clinical impact of miRNA34a and P53 gene expression in colon cancer

miRNA-34a-5p downregulation of VEGFA in endometrial stem cells contributes to the pathogenesis of endometriosis

<p>Overexpression of microRNA-216a inhibits autophagy by targeting regulated MAP1S in colorectal cancer</p>

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