Expression and comparison of sweet corn <scp>CYP81A9s</scp> in relation to nicosulfuron sensitivity

Choe, Eunsoo; Williams, Martin M.

doi:10.1002/ps.5848

Cited by 26 publications

(24 citation statements)

References 40 publications

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“…Whereas, there is only one amino acid substitution in CYP81A21 with no mutations in CYP81A12 between R and S E. phyllopogon plants (Iwakami et al, 2014). Variants of CYP81A9 (Nsf1) have been reported in sweet corn inbred lines, which is responsible for changes in nicosulfuron sensitivity in susceptible lines (Choe and Williams, 2020). In addition to genes from different families or different members from the same family, whether and how these variants affect the level and pattern of herbicide metabolism and thus resistance remain to be determined.…”

Section: Discussionmentioning

confidence: 99%

Cytochrome P450 CYP81A10v7 in Lolium rigidum confers metabolic resistance to herbicides across at least five modes of action

et al. 2020

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SUMMARYRapid and widespread evolution of multiple herbicide resistance in global weed species endowed by increased capacity to metabolize (degrade) herbicides (metabolic resistance) is a great threat to herbicide sustainability and global food production. Metabolic resistance in the economically damaging crop weed species Lolium rigidum is well known but a molecular understanding has been lacking. We purified a metabolic resistant (R) subset from a field evolved R L. rigidum population. The R, the herbicide susceptible (S) and derived F2 populations were used for candidate herbicide resistance gene discovery by RNA sequencing. A P450 gene CYP81A10v7 was identified with higher expression in R vs. S plants. Transgenic rice overexpressing this Lolium CYP81A10v7 gene became highly resistant to acetyl‐coenzyme A carboxylase‐ and acetolactate synthase‐inhibiting herbicides (diclofop‐methyl, tralkoxydim, chlorsulfuron) and moderately resistant to hydroxyphenylpyruvate dioxygenase‐inhibiting herbicide (mesotrione), photosystem II‐inhibiting herbicides (atrazine and chlorotoluron) and the tubulin‐inhibiting herbicide trifluralin. This wide cross‐resistance profile to many dissimilar herbicides in CYP81A10v7 transgenic rice generally reflects what is evident in the R L. rigidum. This report clearly showed that a single P450 gene in a cross‐pollinated weed species L. rigidum confers resistance to herbicides of at least five modes of action across seven herbicide chemistries.

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Section: Discussionmentioning

confidence: 99%

Cytochrome P450 CYP81A10v7 in Lolium rigidum confers metabolic resistance to herbicides across at least five modes of action

et al. 2020

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“…Recently Choe and Williams (2020) confirmed that nsf1 is the gene responsible for sweet corn sensitivity to nicosulfuron. The protein coding sequence of Nsf1 produces CYP81A9; however, the molecular polymorphisms of Nsf1 differ in field corn and sweet corn.…”

Section: Introductionmentioning

confidence: 93%

“…Field corn lines sensitive to nicosulfuron contain either a 392bp insertion mutation in their nsf1 coding sequence, resulting in a truncated, nonfunctional CYP (Williams et al 2006), or two deletion mutations on CYP81A9 (Liu et al 2019). While CYP81A9s from tolerant sweet corn inbreds binds with nicosulfuron, CYP81A9s from sensitive inbreds are inactive, the degree to which is due to the type of amino acid substitutions in CYP81A9 (Choe and Williams 2020).…”

Section: Introductionmentioning

confidence: 99%

Significance of application timing, formulation, and cytochrome P450 genotypic class on sweet corn response to dicamba

et al. 2022

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Sweet corn (Zea mays L.) tolerance to dicamba and several other herbicides is due to cytochrome P450 (CYP) mediated metabolism and is conferred by a single gene (Nsf1). Tolerance varies by CYP genotypic class with hybrids homozygous for functional CYP (Nsf1Nsf1) being the most tolerant and hybrids homozygous for mutant CYP alleles (nsf1nsf1) being the least tolerant. The herbicide safener cyprosulfamide (CSA) increases tolerance to dicamba by stimulating the expression of several CYPs. However, the extent to which CSA improves the tolerance of different sweet corn CYP genotypic classes to dicamba is poorly understood. Additionally, the effect of growth stage on sweet corn sensitivity to dicamba is inadequately described. The objective of this work was to quantify the significance of application timing, formulation, and CYP genotypic class on sweet corn response to dicamba. Hybrids representing each of the three CYP genotypes (Nsf1Nsf1, Nsf1nsf1, nsf1nsf1), were treated with dicamba or dicamba + CSA at one of three growth stages: V3, V6, or V9. Across all timings the nsf1nsf1 hybrid was the least tolerant to dicamba, displaying 16% higher crop injury levels 2 weeks after treatment and 2.13 Mt ha-1 lower ear mass yields compared to the Nsf1Nsf1 hybrid. The V9 growth stage was the most susceptible time for dicamba injury regardless of genotypic class, with 1.89 and 1.75 Mt ha-1 lower ear mass yields compared to the V3 and V6 application timings, respectively. The addition of CSA to dicamba V9 applications reduced the injury from dicamba for all three genotypic classes; however, it did not eliminate the injury. The use of Nsf1Nsf1 or Nsf1nsf1 sweet corn hybrids along with herbicide safeners will reduce the frequency and severity of injury from dicamba and other CYP-metabolized herbicides.

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“…35 In a recent study, nicosulfuron was shown to bind to CYP81A9, although nicosulfuron metabolism was not directly confirmed. 36 Barley CYP81A63 metabolized three herbicides (diclofop-methyl, pinoxaden and tralkoxydim) belonging to three distinct chemical classes of ACCase inhibitors, 37 although the in planta role of this P450 has not been investigated by knock-down or knock-out experiments. The important perspective is that these herbicides are often effective against grass weeds even if grasses carry CYP81A P450s.…”

Section: P450s Involved In Herbicide Selectivitymentioning

confidence: 99%

“…The in planta role of this gene was confirmed by CYP81A9 knock‐down, resulting in the loss of tolerance in the knock‐down plants 35 . In a recent study, nicosulfuron was shown to bind to CYP81A9, although nicosulfuron metabolism was not directly confirmed 36 . Barley CYP81A63 metabolized three herbicides (diclofop‐methyl, pinoxaden and tralkoxydim) belonging to three distinct chemical classes of ACCase inhibitors, 37 although the in planta role of this P450 has not been investigated by knock‐down or knock‐out experiments.…”

Section: P450s Involved In Herbicide Selectivitymentioning

confidence: 99%

Cytochrome P450‐mediated herbicide metabolism in plants: current understanding and prospects

Dimaano

Iwakami

2020

Pest Management Science

118

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Cytochrome P450s (P450s) have been at the center of herbicide metabolism research as a result of their ability to endow selectivity in crops and resistance in weeds. In the last 20 years, ≈30 P450s from diverse plant species have been revealed to possess herbicide-metabolizing function, some of which were demonstrated to play a key role in plant herbicide sensitivity. Recent research even demonstrated that some P450s from crops and weeds metabolize numerous herbicides from various chemical backbones, which highlights the importance of P450s in the current agricultural systems. However, due to the enormous number of plant P450s and the complexity of their function, expression and regulation, it remains a challenge to fully explore the potential of P450-mediated herbicide metabolism in crop improvement and herbicide resistance mitigation. Differences in the substrate specificity of each herbicide-metabolizing P450 are now evident. Comparisons of the substrate specificity and protein structures of P450s will be beneficial for the discovery of selective herbicides and may lead to the development of crops with higher herbicide tolerance by transgenics or genome-editing technologies. Furthermore, the knowledge will help design sound management strategies for weed resistance including the prediction of cross-resistance patterns. Overcoming the ambiguity of P450 function in plant xenobiotic pathways will unlock the full potential of this enzyme family in advancing global agriculture and food security.

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Expression and comparison of sweet corn CYP81A9s in relation to nicosulfuron sensitivity

Cited by 26 publications

References 40 publications

Cytochrome P450 CYP81A10v7 in Lolium rigidum confers metabolic resistance to herbicides across at least five modes of action

Cytochrome P450 CYP81A10v7 in Lolium rigidum confers metabolic resistance to herbicides across at least five modes of action

Significance of application timing, formulation, and cytochrome P450 genotypic class on sweet corn response to dicamba

Cytochrome P450‐mediated herbicide metabolism in plants: current understanding and prospects

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