Expression and distribution of <i>N‐</i>acetyl and <i>N‐</i>glycolylneuraminic acids in secreted and cell‐associated glycoconjugates by two human osteosarcoma cell lines

Tzanakakis, George N.; Nikitovic, Dragana; Katonis, Pavlos; Kanakis, Ioannis; Karamanos, Nikos K.

doi:10.1002/bmc.771

Cited by 13 publications

(10 citation statements)

References 24 publications

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“…The most abundant type was Neu5Ac in both cell-associated and secreted forms followed by Neu5Gc. This data shows different results when compared with the findings in the osteosarcoma cell line, MG-63, which was isolated from patients with different nationality with altered chromosomes 15 . Trace amounts of KDN was also detectable in both osteosarcomas and osteoblasts.…”

Section: Discussioncontrasting

confidence: 65%

“…The main cell-associated Sia found in MG-63 was Neu5Gc, whereas that from the secreted form was Neu5Ac. This indicated that the human osteosarcoma cells expressed both forms of Sia-containing glycoconjugates and the differences in the amounts of each of the two major Sia types and their distribution may be related to the morphology and/or metastatic of osteosarcoma 15 . Another studies of Sia level in the serum reported that the amount of Sia in the serum of osteosarcoma patients was higher than in the control group, but did not differ from those of other bone-forming groups 16 .…”

Section: Discussionmentioning

confidence: 95%

“…The first one was published in Ref. 15 where it was reported that a high metastatic osteosarcoma cell line showed a significant elevation of total Sia when compared with that found in a low metastatic cell line. Moreover, not only was the basic type of Sia (Neu5Ac) found to increase but Neu5Gc also increased.…”

Section: Introductionmentioning

confidence: 99%

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Level and distribution of secreted and cell-associated N-acetyl, N-glycolylneuraminic, and deaminoneuraminic acids in osteosarcoma cells isolated from patients

Phitak¹,

Klangjorhor²,

Pothacharoen³

et al. 2017

ScienceAsia

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Sialic acids, which are members of a family of 9-carbon carboxylated sugars, usually occupy the terminal position of the glycan chains of glycoconjugates and play an important role in various biological processes. Nacetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc) are the dominant sialic acids in mammals, whereas deaminoneuraminic acids (KDN) can be detected in trace amounts. The increase of sialic acid levels has been reported in relation to the pathology and aggressiveness of cancer. Here we used a fluorometric-HPLC method to investigate the level and distribution of Neu5Ac, Neu5Gc, and KDN in osteosarcoma cells isolated from 7 patients and compare them with those in osteoblasts isolated from 3 patients with other bone diseases. Both cell types expressed all three sialic acids with abundances Neu5Ac > Neu5Gc > KDN. Total sialic acid levels in osteosarcoma cells were twice that in osteoblast cells. Only cell-associated Neu5Ac and Neu5Gc levels were higher, but not secreted forms. However, cell-associated and secreted KDN levels were not significantly different among those two groups. This suggests that the level and distribution of sialic acids in osteosarcoma cells were different from those in normal osteoblast cells. Thus their level, type, and distribution may be related to the pathology of osteosarcoma.

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Section: Discussioncontrasting

confidence: 65%

Section: Discussionmentioning

confidence: 95%

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Level and distribution of secreted and cell-associated N-acetyl, N-glycolylneuraminic, and deaminoneuraminic acids in osteosarcoma cells isolated from patients

Phitak¹,

Klangjorhor²,

Pothacharoen³

et al. 2017

ScienceAsia

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“…Both cell lines are of comparable size, yet in vivo MG-63 cells have a higher metastatic potential. [10] In addition, MG-63 cells have a higher proliferation rate (shorter cell cycle time). [11] When grown on microstructured surfaces, both MG-63 and SaOs-2 showed a deformation of their nuclei and their cytoskeleton, visible in the fluorescence microscopy image (Fig.…”

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confidence: 99%

Microstructured Surfaces Cause Severe but Non‐Detrimental Deformation of the Cell Nucleus

Davidson

Özçelik²,

Hasırcı³

et al. 2009

Advanced Materials

112

120

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Surface features on the length scale of organelles allow their manipulation. Here, we present observations of an unexpected deformation of nuclei within cells growing on surfaces with micrometer‐sized pillars. Our results demonstrate that a microstructured surface can induce strong shape deformations in cells, without harmful consequences, and strongly suggest that these are limited to cancerous cells.

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“…In the present study we used human osteosarcoma Mg-63 cells (characterized by a high metastatic potential in vivo) and Soas-2 cells (characterized by a low metastatic potential) (21,22). These two cell lines were purchased from ATCC (LGC Promochem, Wesel, Germany), and were cultured in DMEM medium (Sigma, USA) supplemented with 10% FBS (Amresco, USA) and 1% penicillin/ streptomycin.…”

Section: Methodsmentioning

confidence: 99%

Vasodilator-stimulated phosphoprotein regulates osteosarcoma cell migration

Wei

et al. 2011

Oncol Rep

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Abstract. Vasodilator-stimulated phosphoprotein (VASP) has been reported to play an important role in the process of cell migration and tumor metastasis. However, to date, no study has examined VASP expression and its function in osteocarcoma cells. In this study, we analyzed the effect of VASP on osteosarcoma cell migration and the signal transduction pathways involved. We used two osteosarcoma cell strains (Mg-63 and Saos-2 cells) with different metastatic potential. Silencing of VASP gene expression was carried out using RNA interference in these cells. Knockdown of expression at the transcriptional or translational level was determined by RT-PCR or Western blot analysis, respectively. The metastatic potential of the tumor cells was determined by a wound healing migration assay. VASP mRNA expression was also determined in 30 human osteosarcoma samples. Furthermore, Rac1 was determined as a regulator of VASP function. RT-PCR and Western blotting showed that Mg-63 cells had a significantly higher VASP expression at both the transcriptional and translational levels compared to Saos-2 cells. The wound healing assay revealed that Mg-63 cells had more migratory potential compared to Saos-2 cells. The effect was found to be reversible when VASP was knocked down by siRNA in Mg-63 cells. Specimens from human patients with metastases had higher VASP expression compared to specimens of patients without metastases. Knockdown of Rac1 resulted in inhibition of VASP expression in sarcoma cells. These results suggest that VASP protein regulates osteosarcoma cell migration and metastasis. Rac1 and VASP interaction may be a potential target for osteosarcoma treatment. IntroductionOsteosarcoma is the most prevalent primary malignant bone tumor in children and young adults. Although the life expectancy of osteosarcoma patients has been prolonged due to improvement of neoadjuvant chemotherapy and surgical resection during the past three decades, the long-term survival and quality of life is still unsatisfactory. Poor outcome of osteosarcoma is mainly caused by metastatic disease, and previous studies have demonstrated that more than 30% of patients already display distant metastasis without clinical detectable signs at the time of diagnosis (1,2).Vasodilator-stimulated phosphoprotein (VASP), a member of the Ena/VASP family, has been implicated in regulating key cellular functions, such as shape change, adhesion and migration, which is mainly due to its ability to modify dynamic action cytoskeleton (3). In addition to its role in normal cell growth (4,5), embryonic development (6) and homeostasis (7), VASP plays an essential role in many diseases, such as cancer metastasis (8,9), thrombosis (10), arteriosclerosis (11) and nephritis (12).Expression of VASP has been reported to be dysregulated in several types of malignancies and is relevant to tumor metastasis. For example, its level was found to be significantly increased in lung adenocarcinomas and gastric carcinomas compared to normal tissue, and this increase was correlated wit...

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Expression and distribution of N‐acetyl and N‐glycolylneuraminic acids in secreted and cell‐associated glycoconjugates by two human osteosarcoma cell lines

Cited by 13 publications

References 24 publications

Level and distribution of secreted and cell-associated N-acetyl, N-glycolylneuraminic, and deaminoneuraminic acids in osteosarcoma cells isolated from patients

Level and distribution of secreted and cell-associated N-acetyl, N-glycolylneuraminic, and deaminoneuraminic acids in osteosarcoma cells isolated from patients

Microstructured Surfaces Cause Severe but Non‐Detrimental Deformation of the Cell Nucleus

Vasodilator-stimulated phosphoprotein regulates osteosarcoma cell migration

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