Expression and distribution of pituitary adenylate cyclase-activating peptide in human prostate and prostate cancer tissues

Garcı́a-Fernández, M.Olga; Bodega, Guillermo; Solano, Rosa M.; Ruíz-Villaespesa, Antonio; Sánchez‐Chapado, Manuel; Carmena, Marı́a J.; Prieto, Juan Carlos

doi:10.1016/s0167-0115(02)00108-8

Cited by 16 publications

(11 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Values lower than 200 cells were scored as negative. The selective agonists for VPAC 1 and VPAC 2 receptors, [K 15 , R 16 , L 27 ] VIP (1-7)/GFR (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27) and RO 25-1553, respectively, was a gift from Patrick Robberecht (Université Libre de Bruxelles, Belgium).…”

Section: Detection Of Ne Morphology In Lncap Cellsmentioning

confidence: 99%

Vasoactive intestinal peptide induces neuroendocrine differentiation in the LNCaP prostate cancer cell line through PKA, ERK, and PI3K

et al. 2004

View full text Add to dashboard Cite

show abstract

Section: Detection Of Ne Morphology In Lncap Cellsmentioning

confidence: 99%

Vasoactive intestinal peptide induces neuroendocrine differentiation in the LNCaP prostate cancer cell line through PKA, ERK, and PI3K

et al. 2004

View full text Add to dashboard Cite

show abstract

“…Sections were examined using a Zeiss Axiocam HR camera and AxioVision software 4.8 (Carl Zeiss, Jena, Germany). Healthy human prostate tissue was used as a positive control, as it was previously shown to express PACAP and its 3 receptors [32,33]. …”

Section: Methodsmentioning

confidence: 99%

Distribution and Function of PACAP and Its Receptors in the Healthy and Nephrotic Kidney

Eneman¹,

Heuvel²,

Freson³

et al. 2016

Nephron

View full text Add to dashboard Cite

Background/Aims: Plasma deficiency of pituitary adenylate cyclase-activating polypeptide (PACAP) was recently demonstrated in children with nephrotic syndrome (NS). Previous studies have reported an important protective effect of PACAP on kidney proximal tubules. The aim of this study was to explore the expression of PACAP and its receptors PAC1, VPAC1 and VPAC2 in the healthy and nephrotic kidney and to determine if PACAP has an effect on renal proximal tubular cells exposed to albumin. Methods: Expression of PACAP and its receptors was studied using kidney tissue from healthy and nephrotic children, and in 3 human renal cell lines (glomerular microvascular endothelial cells, podocytes and proximal tubular epithelial HK-2 cells). The functionality of the VPAC1 receptor was tested in HK-2 cells, measuring cyclic adenosine monophosphate levels after PACAP exposure. The influence of PACAP on cell viability and transforming growth factor-β1 (TGF-β1) expression was measured in HK-2 cells exposed to albumin, mimicking proteinuria related damage. Results: VPAC1 expression was detected in the tubular proximal epithelial cells and in the glomerular podocytes of renal tissue from healthy and nephrotic children. Increased staining for PACAP was found in the proximal tubules of renal sections from children with NS compared to healthy renal sections. Expression and functionality of VPAC1 were demonstrated in HK-2 cells. Finally, PACAP did not alter cell viability or TGF-β1 expression of HK-2 cells exposed to albumin. Conclusion: VPAC1 is the predominant receptor in the human kidney. The enhanced presence of PACAP in proximal tubular epithelial cells in nephrotic kidneys points to the reabsorption of filtered PACAP. On short term, PACAP has no in vitro effect on cell viability and TGF-β1 expression of proximal tubular epithelial cells exposed to high concentrations of albumin.

show abstract

“…It was reported that VIP, CGRP and NPY were found in human non-malignant and malignant tissues of the prostate gland and urinary bladder [6]. Similarly, pituitary adenyl cyclase-activating polypeptide (PACAP) is present in tumour tissues of the ovary, prostate gland and mammary gland [7][8][9]. In these papers except for confirmation of the presence of PACAP, VIP and NPY, there is no explanation of the possible role of neuropeptides mentioned above in carcinogenesis of these organs.…”

Section: Introductionmentioning

confidence: 98%

Concentration of vasoactive intestinal polypeptide, neuropeptide Y and calcitonin gene related peptide in neoplastic tissue of the human large intestine

Godlewski¹,

Pidsudko²,

Sienkiewicz³

2011

View full text Add to dashboard Cite

A Ai im m o of f t th he e s st tu ud dy y: : The purpose of this study was to investigate the presence of vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY) and calcitonin gene related peptide (CGRP) neuropeptides in neoplastic tissue of the large intestine. The results obtained were compared to an unchanged part of the intestine originating from the same patient. M Ma at te er ri ia al l a an nd d m me et th ho od ds s: : The tissue samples were taken from the postoperative part of the intestine, resected during an operation due to treatment of colon cancer. Tissues were taken from 15 patients. Concentrations of neuropeptides were determined by ELISA tests, and results were calculated per 1 g of fresh tissue. The data obtained were statistically analysed. R Re es su ul lt ts s: : In tissues of the neoplastic tumour of the sigmoid colon and rectum the concentration of VIP and NPY was determined (28.9 ng/g and 8.6 ng/g, respectively). The observed values were statistically significantly lower than those observed in corresponding tissues of unchanged intestinal segments (83.9 ng/g for VIP and 19.5 ng/g for NPY). Surprisingly, amounts of CGRP were very similar in both neoplastic and intact tissues (13.3 ng/g and 13.4 ng/g respectively). C Co on nc cl lu us si io on ns s: : Immunoenzymatic investigations allow one to establish the concentration of neuropeptides associated with the enteric nervous system in cancer tissues of the large intestine. Various concentrations of these neuropeptides were observed in particular patients of both compared groups. The performed statistical analysis did not reveal associations between degree of cancer infiltration within the colonic wall and the level of presence of investigated neuropeptides in either cancer or control tissues. The fact of presence of these neuropeptides in cancer tissues confirmed by this study should be underlined at the same time.K Ke ey y w wo or rd ds s: : colon cancer, enteric nervous system, neuropeptides, calcitonin gene related peptide, neuropeptide Y, vasoactive intestinal polypeptide.

show abstract

Expression and distribution of pituitary adenylate cyclase-activating peptide in human prostate and prostate cancer tissues

Cited by 16 publications

References 26 publications

Vasoactive intestinal peptide induces neuroendocrine differentiation in the LNCaP prostate cancer cell line through PKA, ERK, and PI3K

Vasoactive intestinal peptide induces neuroendocrine differentiation in the LNCaP prostate cancer cell line through PKA, ERK, and PI3K

Distribution and Function of PACAP and Its Receptors in the Healthy and Nephrotic Kidney

Concentration of vasoactive intestinal polypeptide, neuropeptide Y and calcitonin gene related peptide in neoplastic tissue of the human large intestine

Contact Info

Product

Resources

About