Expression and distribution of SIBLING proteins in the predentin/dentin and mandible of hyp mice

Zhang, B; Sun, Yao; Chen, L; Chen, Guan; Guo, Lei; Qin, Chunlin

doi:10.1111/j.1601-0825.2010.01656.x

Cited by 32 publications

(32 citation statements)

References 62 publications

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“…It has also been suggested that PHEX is likely responsible for the cleavage of DMP1 and DSPP, as it has a strong preference for cleaving bonds at the N-terminal of these two SIBLING proteins . However, analysis of the Hyp mouse indicated no differences in Dmp1 and Dspp expression in comparison with their WT controls, suggesting that DMP1 and DSPP are in fact properly processed in the Phex-deficient mouse (Zhang et al 2010). In addition to this, there is an accumulation of SIBLING ASARM peptides in the Hyp mouse and patients, thus challenging the hypothesis that the SIBLING proteins are substrates for PHEX.…”

Section: The Asarm Hypothesis and Bone Diseasesmentioning

confidence: 59%

The importance of the SIBLING family of proteins on skeletal mineralisation and bone remodelling

Staines¹,

MacRae²,

Farquharson³

2012

Journal of Endocrinology

192

109

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The small integrin-binding ligand N-linked glycoprotein (SIBLING) family consists of osteopontin, bone sialoprotein, dentin matrix protein 1, dentin sialophosphoprotein and matrix extracellular phosphoglycoprotein. These proteins share many structural characteristics and are primarily located in bone and dentin. Accumulating evidence has implicated the SIBLING proteins in matrix mineralisation. Therefore, in this review, we discuss the individual role that each of the SIBLING proteins has in this highly orchestrated process. In particular, we emphasise how the nature and extent of their proteolytic processing and post-translational modification affect their functional role. Finally, we describe the likely roles of the SIBLING proteins in clinical disorders of hypophosphataemia and their potential therapeutic use.

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Section: The Asarm Hypothesis and Bone Diseasesmentioning

confidence: 59%

The importance of the SIBLING family of proteins on skeletal mineralisation and bone remodelling

Staines¹,

MacRae²,

Farquharson³

2012

Journal of Endocrinology

192

109

View full text Add to dashboard Cite

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“…It has been reported that the NH 2 -terminal fragments of DMP-1 localized to predentin and not mineralized dentin matrix in rat molar [21], indicating that it as an inhibitor of dentin mineralization. Zhang et al [22] demonstrated that DMP-1 N-terminal fragments were located not only in normal rat predentin, but also in widened areas of predentin in Hyp mouse. Therefore, another possible explanation for the formation of a large number of unmineralized areas in the dentin observed here is that amoxicillin injection may directly and/or indirectly affect the SIBLING family, including DMP-1, leading to disruption of dentin mineralization, as suggested by Boukpessi et al [20].…”

Section: Discussionmentioning

confidence: 98%

Effect of single-dose amoxicillin on rat incisor odontogenesis: a morphological study

et al. 2011

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The effect of exposure to amoxicillin on tooth development remains to be elucidated. The purpose of this study was to investigate the effect of amoxicillin on rat incisor odontogenesis. Male Wistar rats weighing approximately 100 g were given a single intraperitoneal injection of 3.0 g/kg body weight amoxicillin. One week after injection, the rats were fixed, and the lower incisors were demineralized and prepared into paraffin sections for light microscopy (LM) and immunohistochemistry. Undemineralized samples were embedded in resin and ground for processing for contact microradiography (CMR) and scanning electron microscopy (SEM). Serum calcium, phosphate, and magnesium concentrations were measured. At 1 week after amoxicillin administration, LM, CMR, and SEM revealed a clear increase in the area of interglobular dentin, representing disruption of mineralization by odontoblasts. Immunohistochemistry demonstrated moderate levels of the small integrin-binding ligand N-linked glycoprotein family dentin matrix protein 1 in large areas of interglobular dentin. On the other hand, no morphological alteration or hypomineralization was observed in the enamel. Serum calcium values showed no significant differences between the control and experimental rats during the experimental period although both serum phosphate and magnesium levels increased at day 1 after amoxicillin injection. The results suggest that a single dose of amoxicillin specifically affects normal tooth dentin mineralization, but not enamel mineralization in rat incisor odontogenesis. The present results further our understanding of the clinical association between dentin abnormality and amoxicillin exposure during tooth development.

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“…1) The forming alveolar bone cells originate from the neural crest, which is from the ectoderm, while the osteoblasts of the long bone are from the mesoderm. 2) The DSPP expression level in alveolar bone is much higher than in the long bone [19, 45]. 3) The alveolar bone defects in Dspp -null mice [28] are much more severe than the long bone defects [22], suggesting that DSPP’s role in alveolar bone formation is more important than in the long bone.…”

Section: Discussionmentioning

confidence: 99%

Transgenic expression of Dspp partially rescued the long bone defects of Dmp1-null mice

Jani¹,

Gibson²,

Liu³

et al. 2016

Matrix Biology

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Dentin matrix protein 1 (DMP1) and dentin sialophosphoprotein (DSPP) belong to the Small Integrin-Binding Ligand N-linked Glycoprotein (SIBLING) family. In addition to the features common to all SIBLING members, DMP1 and DSPP share several unique similarities in chemical structure, proteolytic activation and tissue localization. Mutations in, or deletion of DMP1, cause autosomal recessive hypophosphatemic rickets along with dental defects; DSPP mutations or its ablation are associated with dentinogenesis imperfecta. While the roles and functional mechanisms of DMP1 in osteogenesis have been extensively studied, those of DSPP in long bones have been studied only to a limited extent. Previous studies by our group revealed that transgenic expression of Dspp completely rescued the dentin defects of Dmp1-null (Dmp1−/−) mice. In this investigation, we assessed the effects of transgenic Dspp on osteogenesis by analyzing the formation and mineralization of the long bones in Dmp1−/− mice that expresses a transgene encoding full-length DSPP driven by a 3.6-kb rat Col1a1 promoter (referred as “Dmp1−/−;Dspp-Tg mice”). We characterized the long bones of the Dmp1−/−;Dspp-Tg mice at different ages and compared them with those from Dmp1−/− and Dmp1+/− (normal control) mice. Our analyses showed that the long bones of Dmp1−/−;Dspp-Tg mice had a significant increase in cortical bone thickness, bone volume and mineral density along with a remarkable restoration of trabecular thickness compared to those of the Dmp1−/− mice. The long bones of Dmp1−/−;Dspp-Tg mice underwent a dramatic reduction in the amount of osteoid, significant improvement of the collagen fibrillar network, and better organization of the lacunocanalicular system, compared to the Dmp1−/− mice. The elevated levels of biglycan, bone sialoprotein and osteopontin in Dmp1−/− mice were also noticeably corrected by the transgenic expression of Dspp. These findings suggest that DSPP and DMP1 may function synergistically within the complex milieus of bone matrices.

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Expression and distribution of SIBLING proteins in the predentin/dentin and mandible of hyp mice

Cited by 32 publications

References 62 publications

The importance of the SIBLING family of proteins on skeletal mineralisation and bone remodelling

The importance of the SIBLING family of proteins on skeletal mineralisation and bone remodelling

Effect of single-dose amoxicillin on rat incisor odontogenesis: a morphological study

Transgenic expression of Dspp partially rescued the long bone defects of Dmp1-null mice

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