Expression and function of the LIM-homeodomain transcription factor Islet-1 in the developing and mature vertebrate retina

Bejarano-Escobar, Ruth; Álvarez-Hernán, Guadalupe; Morona, Ruth; González, Agustı́n; Martı́n-Partido, Gervasio; Francisco-Morcillo, Javier

doi:10.1016/j.exer.2015.06.021

Cited by 29 publications

(34 citation statements)

References 87 publications

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“…52,53,[68][69][70] It also extended to more peripheral regions of the retina following a central to peripheral gradient, similar to the central-to-peripheral sweep of cell differentiation described by the aforecited authors in the chick retina. 52,53,[68][69][70] It also extended to more peripheral regions of the retina following a central to peripheral gradient, similar to the central-to-peripheral sweep of cell differentiation described by the aforecited authors in the chick retina.…”

Section: Sa-β-gal Activity In the Nblmentioning

confidence: 76%

“…[52][53][54] At HH18, a faint SA-β-GAL histochemical signal was observed in the presumptive ganglion cell layer (GCL), colocalizing with TUJ-1 immunoreactivity in differentiating neurons ( Figure 2C-D). [52][53][54] At HH18, a faint SA-β-GAL histochemical signal was observed in the presumptive ganglion cell layer (GCL), colocalizing with TUJ-1 immunoreactivity in differentiating neurons ( Figure 2C-D).…”

Section: Resultsmentioning

confidence: 99%

“…TUJ-1 and islet-1 (Isl1) have been described as excellent markers of the first differentiating neurons in the developing avian retina. [52][53][54] At HH18, a faint SA-β-GAL histochemical signal was observed in the presumptive ganglion cell layer (GCL), colocalizing with TUJ-1 immunoreactivity in differentiating neurons ( Figure 2C-D). At this stage, some differentiating Isl1-positive migrating progenitors were detected in the vitreal half of the NbL in the central retina ( Figure 2E, F), coinciding with the location of SA-β-GAL staining.…”

Section: Resultsmentioning

confidence: 99%

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Senescence‐associated β‐galactosidase activity in the developing avian retina

Mera-Rodríguez

Álvarez-Hernán

Gañán

et al. 2019

Developmental Dynamics

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Background: Senescence-associated β-galactosidase (SA-β-GAL) histochemistry is the most commonly used biomarker of cellular senescence. These SA-β-GALpositive cells are senescent embryonic cells that are usually removed by apoptosis from the embryo, followed by macrophage-mediated clearance. Results: Some authors have proposed that SA-β-GAL activity in differentiated neurons from young and adult mammals cannot be uniquely attributed to cell senescence, whether in vivo or in vitro. Using the developing visual system of the chicken as a model, the present study found that SA-β-GAL detected in the developing retina corresponded to lysosomal β-galactosidase activity, and that SAβ-GAL activity did not correlate with the chronotopographical distribution of apoptotic cells. However, SA-β-GAL staining in the undifferentiated retina coincided with the appearance of early differentiating neurons. In the laminated retina, SAβ-GAL staining was concentrated in the ganglion, amacrine, and horizontal cell layers. The photoreceptors and pigment epithelial cells also exhibited SA-β-GAL activity throughout retinal development. We have also found that SA-β-GAL staining strongly correlated p21 immunoreactivity. Conclusion: In conclusion, the results clearly show that SA-β-GAL activity cannot be regarded as a specific marker of senescence during retinal development, and that it is mainly expressed in subpopulations of postmitotic neurons, which are nonproliferative cells, even at early stages of cell differentiation. K E Y W O R D Sapoptosis, cell differentiation, chicken embryo, retina, senescence, senescence-associated β-galactosidase

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Section: Sa-β-gal Activity In the Nblmentioning

confidence: 76%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Senescence‐associated β‐galactosidase activity in the developing avian retina

Mera-Rodríguez

Álvarez-Hernán

Gañán

et al. 2019

Developmental Dynamics

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“…Several lines of evidence suggest that LIM codes control various functions in differentiating neurons, since the positioning of cell bodies, axon projections, neurotransmitter identities and ion channel expression, are all affected in many different neurons when ISL1 is downregulated9234849505152. Both ISL1 and LHX4 appeared to be required to induce Slit2 transcription as shown in cell lines, chick and mouse embryos, which favors the idea that they constitute the LIM code.…”

Section: Discussionmentioning

confidence: 89%

ISL1-based LIM complexes control Slit2 transcription in developing cranial motor neurons

Kim

et al. 2016

Sci Rep

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LIM-homeodomain (HD) transcription factors form a multimeric complex and assign neuronal subtype identities, as demonstrated by the hexameric ISL1-LHX3 complex which gives rise to somatic motor (SM) neurons. However, the roles of combinatorial LIM code in motor neuron diversification and their subsequent differentiation is much less well understood. In the present study, we demonstrate that the ISL1 controls postmitotic cranial branchiomotor (BM) neurons including the positioning of the cell bodies and peripheral axon pathfinding. Unlike SM neurons, which transform into interneurons, BM neurons are normal in number and in marker expression in Isl1 mutant mice. Nevertheless, the movement of trigeminal and facial BM somata is stalled, and their peripheral axons are fewer or misrouted, with ectopic branches. Among genes whose expression level changes in previous ChIP-seq and microarray analyses in Isl1-deficient cell lines, we found that Slit2 transcript was almost absent from BM neurons of Isl1 mutants. Both ISL1-LHX3 and ISL1-LHX4 bound to the Slit2 enhancer and drove endogenous Slit2 expression in SM and BM neurons. Our findings suggest that combinations of ISL1 and LHX factors establish cell-type specificity and functional diversity in terms of motor neuron identities and/or axon development.

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“…As a pleiotropic transcription factor, Islet‐1 targets multiple downstream genes in temporally and spatially specific manners. Islet‐1 plays an important role in the development of the pancreas , progenitors of a majority of hindlimb cells , vertebrate retina , and inner ear; in motor column formation; and in the transition from sensory neurogenesis to subtype specification . Additionally, as a cardiac progenitor cell marker, Islet‐1 is expressed in the second heart field and is very important in the development of the heart.…”

Section: Discussionmentioning

confidence: 99%