Expression and immunohistochemical localization of the cytochrome P450 isoform 356A1 (CYP356A1) in oyster Crassostrea gigas

Rodrigues-Silva, Christielly; Flores-Nunes, Fabrício; Vernal, Javier; Cargnin-Ferreira, Eduardo; Bainy, Afonso Celso Dias

doi:10.1016/j.aquatox.2014.12.021

Cited by 13 publications

(3 citation statements)

References 42 publications

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“…These last years saw a significant advance in oyster genomic resources (Zhang et al 2012). A gene belonging to the CYP sub-family, CYP356A1, was identified in oysters exposed to a sanitary sewage-effluent (Rodrigues-Silva et al 2015). Through the production of a CYP356A1 purified protein and a corresponding mouse polyclonal antibody, dot blot analysis highlighted the inducibility of the CYP proteins in the lining epithelial cells of mantle, stomach, intestine, and gills but also in free cells such as hemocytes.…”

Section: Fate Of Diuron In Oyster and Detoxification Processmentioning

confidence: 99%

Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts

Akcha

Barranger

Bachère

et al. 2016

Environ Sci Pollut Res

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Genitors of the Pacific oyster Crassostrea gigas were submitted during gametogenesis to a short pulse exposure to the herbicide diuron at a realistic environmental concentration. Histological analysis showed no effect of diuron on gametogenesis course, sex ratio and reproductive effort. A non-significant increase in testosterone and progesterone levels was observed in genitors exposed to the herbicide. At cell level, diuron exposure was shown to modulate the phagocytic activity of circulating hemocytes. The results of a transcriptional analysis showed that diuron affected the expression of genes belonging to functions known to play a major role during oyster gametogenesis such as gene transcription regulation, DNA replication and repair, DNA methylation and cytokinesis. Taking into account the results we previously obtained on the same genitors, this study showed a negative effect of diuron on oyster reproduction by inducing both structural and functional modifications of the DNA.

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Section: Fate Of Diuron In Oyster and Detoxification Processmentioning

confidence: 99%

Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts

Akcha

Barranger

Bachère

et al. 2016

Environ Sci Pollut Res

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“…The Pacific oyster is a typical sentinel organism for biomonitoring studies and is widely used to evaluate environmental pollutant effects since it can accumulate and tolerate these compounds141516. Previous studies have demonstrated upregulation of C. gigas FABP intestinal-like gene (GenBank accession ABU41520) after sewage and pharmaceutical exposures17181920.…”

mentioning

confidence: 99%

Intracellular lipid binding protein family diversity from Oyster Crassostrea gigas: genomic and structural features of invertebrate lipid transporters

Toledo-Silva

Razzera

Zacchi

et al. 2017

Sci Rep

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Intracellular lipid binding proteins (iLBPs) play a role in the transport and cellular uptake of fatty acids and gene expression regulation. The aim of this work was to characterize the iLBP gene family of the Pacific oyster Crassostrea gigas, one of the most cultivated marine bivalves in the world, using bioinformatics and molecular biology approaches. A total of 26 different iLBPs transcripts were identified in the Pacific oyster genome, including alternative splicing and gene duplication events. The oyster iLBP gene family seems to be more expanded than in other invertebrates. Furthermore, 3D structural modeling and molecular docking analysis mapped the main amino acids involved in ligand interactions, and comparisons to available protein structures from vertebrate families revealed new binding cavities. Ten different CgiLBPs were analyzed by quantitative PCR in various tissues of C. gigas, which suggested differential prevalent gene expression of CgiLBPs among tissue groups. The data indicate a wider repertoire of iLBPs in labial palps, a food-sorting tissue. The different gene transcription profiles and reported docking systems suggest that the iLBPs are a non-generalist ligand binding protein family with specific functions.

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“…Estas proteínas se han reportado en la membrana del retículo endoplásmico hepático de peces y en el hepatopáncreas de moluscos bivalvos (Zhang et al, 2012). Se ha empleado el uso de CYP como una herramienta para la evaluación ambiental utilizando hidrocarburos policíclicos aromáticos (HAP) y metales pesados (Pan et al, 2011;Rodrigues-Silva et al, 2015;Zhang et al, 2012) y se ha observado la sobreexpresión de CYP en hemocitos de la almeja Venerupis philippinarum recolectada en zonas contaminadas por hidrocarburos (Boscolo Papo et al, 2014a). La almeja P. caroliniana de Tecolutla, Veracruz, mostró inmunorreacción positiva en las glándulas digestivas, órganos asociados a la digestión y desintoxicación de contaminantes (Morse y Zardus, 1997;Robledo et al, 1997).…”

Section: Discussionunclassified

Análisis de biomarcadores en la almeja Polymesoda caroliniana para la generación de un índice de salud y vulnerabilidad

Juárez

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Expression and immunohistochemical localization of the cytochrome P450 isoform 356A1 (CYP356A1) in oyster Crassostrea gigas

Cited by 13 publications

References 42 publications

Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts

Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts

Intracellular lipid binding protein family diversity from Oyster Crassostrea gigas: genomic and structural features of invertebrate lipid transporters

Análisis de biomarcadores en la almeja Polymesoda caroliniana para la generación de un índice de salud y vulnerabilidad

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