Expression and kinetic analysis of carboxylesterase LmCesA1 from Locusta migratoria

Yin, Fei; Ma, Wei; Li, Daqi; Zhang, Xueyao; Zhang, Jianqin

doi:10.1007/s10529-021-03086-1

Biotechnol Lett

2021

DOI: 10.1007/s10529-021-03086-1

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Expression and kinetic analysis of carboxylesterase LmCesA1 from Locusta migratoria

Fei Yin

Wei Ma

Daqi Li

et al.

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2024

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Activities and Kinetic Parameters of Carboxylesterases in Model Insects depending on a Substrate of the Enzyme

Kinareikina,

Silivanova

2024

Agrohimiâ

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House flies Musca domestica L. (Diptera: Muscidae) serve as a common model organism for testing of insecticides and research of insecticidal resistance mechanisms in insects. One of important stages is to assess of detoxifying enzyme activities including carboxylesterase activities (CarE). In this study, we compared specific activities and kinetic parameters (Vmax and Km) of CarE in adults M. domestica of two laboratory strains (TY, UF) depending on the enzymatic substrate used. The specific CarE activities towards α- and β-naphthyl acetate (α-NA and β-NA) were similar in both males and females of the TY strain. In males of the UF strain, the value of the specific and the maximal velocity (Vmax) of β-NA hydrolysis was 1.90- and 1.57-fold respectively less than that of α-NA; this difference was not observed in females of the same strain. Some characteristics of CarE varied depending on sex of insects when p-nitrophenyl acetate was used as an enzymatic substrate. In particular, the specific activity was 1.62-fold less in males of the UF strain compared to this value in females. The activity and main kinetic parameters of CarE towards α-NA not differed statistically significant depending on sex and the strains. Based on the results obtained we suggest that α-naphthyl acetate is the preferred substrate to evaluate the CarE enzymatic activity in the model insect M. domestica of different strains.

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Activities and Kinetic Parameters of Carboxylesterases in Model Insects depending on a Substrate of the Enzyme

Kinareikina,

Silivanova

2024

Agrohimiâ

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show abstract

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Expression and kinetic analysis of carboxylesterase LmCesA1 from Locusta migratoria

Cited by 1 publication

References 33 publications

Activities and Kinetic Parameters of Carboxylesterases in Model Insects depending on a Substrate of the Enzyme

Activities and Kinetic Parameters of Carboxylesterases in Model Insects depending on a Substrate of the Enzyme

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