Expression and Localization of Phosphoinositide-Specific Phospholipases C in Cultured, Differentiating and Stimulated Human Osteoblasts

Specific Subcellular Localization of Phosphoinositide-Specific Phospholipase C enzymes in Different Human Osteosarcoma Cell Lines

“…Selected PLC enzymes were detected in pseudopodlike structures, in cell protrusions, and/or in membrane ruffles (Table 3, Figures 2 and 3). This observation accords to previous literature data describing PLC enzymes in pseudopod-like structures [39,40,46,47], probably suggesting that PLC enzymes might be involved in in cell cytoskeleton reorganization. The presence and distribution of pseudopodlike structures we identified in the present experiments increased depending on the malignancy potential of the analyzed OS cells lines.…”

“…Selected PLC enzymes were detected in intracellular vesicles in all the analyzed OS cell lines, accordingly to previous literature data that identified the presence of PLCs both in intracellular vesicles in osteoblasts [39,40] and in extracellular vesicles in different OS cell lines [17].…”

“…In our present experiments, MG-63 cells lack the presence of selected PLC enzymes, including PLC δ3. Recent literature data report that PLC δ3 was not detected in human osteoblasts [39,40]. PLC δ3 in involved in physiological processes, such as the downregulation of RhoA/Rho signaling during neurite extension [41], and in cardiomyocyte survival [42].…”