Expression and modulation by cytokines of the intercellular adhesion molecule‐1 (ICAM‐1) in human central nervous system tumor cell cultures

Abstract

The intercellular adhesion molecule (ICAM-1) has been shown to be important in interactions involving cells of the immune system and to be upregulated in a number of cell culture systems by cytokines, including immune interferon (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha). In the present study, we have determined by fluorescence-activated cell sorter (FACS) analysis and the anti-ICAM-1 monoclonal antibody (MAb) CL203.4 the base-line expression of ICAM-1 and its modulation by recombinant IFN-beta, IFN-gamma and TNF-alpha in early passage (less than 15) human central nervous system (CNS) tumor-derived cell cultures. These cultures were established from various malignancies, including glioblastoma multiforme (GBM), astrocytoma (AST), ganglioglioma, medulloblastoma, meningioma and a pineal tumor. ICAM-1 expression was highest in the GBM- and AST-derived cell cultures and was lowest in the ganglioglioma and normal pineal cell cultures. Variable ICAM-1 expression was found, however, in tumors of the same histological group. In several cell cultures the variable expression observed by FACS was substantiated by the intensity of the molecular species immunoprecipitated by the anti-ICAM-1 MAb CL203.4 from these cells. All the cell cultures displayed variable but consistent increases in ICAM-1 expression following treatment with IFN-gamma or TNF-alpha. In general, the degree of increase in ICAM-1 expression was greatest in cultures exposed to TNF-alpha. Upregulation of ICAM-1 expression in an established glioblastoma multiforme cell line was of greater magnitude and more rapid following TNF-alpha treatment (within 2 to 3 hr) than exposure to IFN-gamma (by 24 hr). In several cultures, IFN-beta also increased ICAM-1 expression and enhanced the increase induced by TNF-alpha. The results of the present study indicate that variable expression of ICAM-1 is a common property of early passage cultures of CNS tumors and recombinant interferons and TNF-alpha can differentially upregulate ICAM-1 expression in these CNS tumor cell cultures.

Insights into glutamate transport regulation in human astrocytes: Cloning of the promoter for excitatory amino acid transporter 2 (EAAT2)

“…HCN-2, HCN-1A, DU-145, MCF-7, Colo 205, and PANC-1 cells were from the American Type Culture Collection and maintained as described (25). A normal cerebellum cell line was established and grown as described (27).…”

Genomic structure, chromosomal localization and expression profile of a novel melanoma differentiation associated (mda-7) gene with cancer specific growth suppressing and apoptosis inducing properties

“…HBL-100, MCF-7, T47D, MDA-MB-157, MDA-MB-231, MDA-MB-453, LNCaP, PC-3, DU-145, HeLa, Saos2, MIA PaCa-2, PANC-1, BxPC-3, AsPC-1, T98G and HONE-1 cells were obtained from the American Type Culture Collection. NC, a normal human cerebellum astrocyte cell line and the GBM-18 human glioblastoma multiforme cell line were established in culture from patient-derived samples (Vita et al, 1988;Guarini et al, 1990). Early passage normal human prostate epithelial cells (HuPEC) were obtained from Clonetics Inc. (CA, USA) and cultured using reagents and medium provided by the company.…”

Levels of soluble intercellular adhesion molecule‐1 and total sialic acid in serum of patients with colorectal cancer

“…ICAM-1, the inducible ligand for lymphocyte function associated antigen-1 (LFA-1) and Mac-1, is found on endothelial cells, leukocytes, and some epithelial tissue, and plays a major role in cell-cell interactions in inflammatory and immune response [5][6][7]. In previous reports, an elevation of serum ICAM-1 or soluble variant of ICAM-1 (sICAM-1) was seen in patients with gastric and pancreatic carcinoma [1,8,9], melanoma, metastatic cancer and urolithiasis [6], systemic lupus erythematosus [10]. In addition, the concentrations were found to be related with staging and metastasis [1].…”