Expression and production of soluble Mycobacterium tuberculosis H37Rv mycosin-3

Fang, Zhuo; Schubert, Wolf‐Dieter; Pittius, Nicolaas C. Gey van

doi:10.1016/j.bbrep.2016.02.005

Cited by 1 publication

(2 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The membrane-anchored mycosin genes encode a family of subtilisin-like proteases, designated MycP1-MycP5 (Brown et al, 2000;Fang et al, 2016). These genes comprise a part of the ESX system that is involved in the virulence of MTBC and they are also present in BCG strains.…”

Section: Genomic Analysismentioning

confidence: 99%

See 1 more Smart Citation

Phylogenomic and epidemiological insights into two clinical Mycobacterium bovis BCG strains circulating in South Africa

Modipane

Reva

Magazi

et al. 2019

International Journal of Infectious Diseases

View full text Add to dashboard Cite

Background: Mycobacterium bovis BCG is a live, attenuated tuberculosis vaccine. While the vaccine protects infants from tuberculosis, complications including disseminated infections have been reported following vaccination. Genetically diverse BCG sub-strains now exist following continuous passaging of the original Pasteur strain for vaccine manufacture. This genetic diversity reportedly influences the severity of disseminated BCG infections and the efficacy of BCG immunization. Methods: M. bovis BCG was isolated from infants suspected of being infected with tuberculosis. The whole genome of the clinical isolates and BCG Moscow were sequenced using Illumina Miseq and the sequences were analysed using CLC Genomics Workbench 7.0, PhyResSE v1.0, and Parsnp. Results and conclusions: Genetic variations between the clinical strains and the reference BCG Copenhagen were identified. The clinical strains shared only one mutation in a secretion protein. Mutations were identified in various antibiotic resistance genes in the BCG isolates, which suggests their potential as multidrug-resistant (MDR) phenotypes. Phylogenetic analysis showed that the two isolates were distantly related, and the M1_S48 clinical isolate was closely related to M. bovis BCG Moscow. The phylogenomics results imply that two different BCG strains may be circulating in South Africa. However, it is difficult to associate the BCG vaccine strain administered and the BCG strain supplied with specific adverse events, as BCGiosis is under-reported. This study presents background genomic information for future surveillance and tracking of the distribution of BCGiosis-associated mycobacteria. It is also the first to report on the genomes of clinical BCG strains in Africa.

show abstract

Section: Genomic Analysismentioning

confidence: 99%

“…Additionally, MycP5 reportedly facilitates the secretion of the PE/PPE (prolineglutamate/proline-proline-glutamate) proteins. Mutations in these genes may therefore interfere with mycobacterial growth and secretions of the PE/PPE proteins (Chen, 2016;Fang et al, 2016). The PE-PGRs are a subfamily of the PE proteins.…”

Section: Genomic Analysismentioning

confidence: 99%