Expression and prognostic significance of Livin in gastric cancer

Abstract

Livin is one of the most important members of the inhibitor of apoptosis protein family. It is overexpressed in several types of tumors and may have prognostic significance. The present study investigated the biological role of Livin in the oncogenic behavior of gastric cancer cells, the expression of Livin in gastric cancer tissue and the relationship of its expression with various clinicopathological parameters and patient survival. Small interfering RNA blocked Livin gene expression in AGS and SNU638 human gastric cancer cell lines. The expression of Livin was investigated in gastric cancer tissues by RT-PCR, western blotting and immunohistochemistry. The associations with various clinicopathological parameters and survival were analyzed. Livin knockdown inhibited tumor cell migration, invasion and proliferation in AGS and SNU638 cells. Livin knockdown induced apoptosis by activating caspase-3, caspase-7 and PARP. Livin knockdown induced cell cycle arrest by a decrease in cyclin D1, cyclin-dependent kinase 4 and 6 and an increase in expression of p21 and p27. The ERK1/2 and JNK signaling pathways were inhibited by Livin knockdown. Livin expression was upregulated in gastric cancer tissues at the mRNA and protein levels. However, no significant correlation was found between Livin expression and various clinicopathological parameters including survival. In conclusion, Livin expression may be important in the alteration of invasive and oncogenic phenotypes of gastric cancer cells. The prognostic relevance of Livin remains unclear.

Livin/BIRC7 expression as malignancy marker in adrenocortical tumors

“…Similarly, in a very recent multicentric study regarding a comprehensive genomic characterization of a large series of 80 ACC [39], the authors found a relatively high livin mRNA expression by RNA-sequencing (median log fold change = 5.96). All these findings suggest a potential major role of livin in the adrenocortical tumor development, as previously reported for other cancer types [18–20, 29, 40–42]. …”

“…We did not found any significant correlation between livin expression at both mRNA and protein levels, and the histopathological and/or clinical parameters, including the clinical outcome as observed in some [22, 27, 42], but not all other cancer types [20, 29, 44, 45]. Our results are in accordance to other recently published gene expression data in ACCs [39], where no significant relationship was observed between livin expression and overall survival ( p = 0.86 ).…”

“…Livin up-regulation is mainly a risk factor for cancer progression, poor prognosis and resistance to anti-tumor treatment [24, 25]. However, in some tumors, such as in childhood acute lymphoblastic leukemia, malignant mesothelioma, renal cell carcinoma and hepatocellular carcinoma, high livin expression is correlated with better prognosis [23, 26–28] or has no impact on clinical outcome [20, 29]. Furthermore, several studies demonstrated a negative correlation between the expression of livin and caspase-3 [24, 30, 31], which represents the main molecular target of livin action.…”

Effects of siRNA Livin on EJ human bladder cancer cells treated with mitomycin-C

“…Overexpression of Livin inhibits the apoptosis of tumor cells and leads to the resistance of tumors to pro-apoptotic factors (11,12). In addition, Livin is associated with the invasiveness of tumors and oncogenic phenotypes (13). These studies suggest that Livin is one potential targeting site for the therapy of bladder carcinoma.…”

“…Therefore, finding new targeting sites and enhancing chemotherapeutic sensitivity are critical for the therapy of bladder carcinoma. Previous studies indicated that Livin is not expressed or expressed at low levels in most differentiated terminal tissues of adults, but overexpressed in a number of malignant tumors (8)(9)(10). Overexpression of Livin inhibits the apoptosis of tumor cells and leads to the resistance of tumors to pro-apoptotic factors (11,12).…”