Expression and prognostic significance of centromere protein A in human lung adenocarcinoma

Short hairpin RNA-mediated down-regulation of CENP-A attenuates the aggressive phenotype of lung adenocarcinoma cells

“…Some commercial RNA-based assays include CENP-A as a biomarker for cancer prognosis. Previously, we found that CENP-A expression is significantly increased in lung adenocarcinoma and has a poor prognostic impact on the overall survival of patients with lung adenocarcinoma [12]. Here, we confirm a high level of expression of CENP-A in at least some of the lung adenocarcinoma-derived cell lines tested.…”

“…Our previous work has shown that elevated CENP-A expression is associated with the occurrence of aggressive tumor phenotypes and poor overall survival rates in patients with lung adenocarcinoma [12]. These clinical findings suggest an important role for CENP-A in lung cancer progression.…”

“…mRNA expression analysis was performed using reverse transcription-polymerase chain reaction (RT-PCR) in conjunction with quantitative PCR (qPCR), as described before [12][13][14]. Briefly, total RNA was extracted from cells or tissues using an RNeasy mini kit (Qiagen, Hilden, Germany) and complementary DNA (cDNA) was synthesized using a First-Strand cDNA Synthesis Kit for RT-PCR (Roche, Mannheim, Germany).…”

Essential role for centromeric factors following p53 loss and oncogenic transformation

“…CENP-A overexpression has been reported in several human cancers, including breast (Ma et al 2003;Montes de Oca et al 2015), colorectal (Tomonaga et al 2003), liver (Li et al 2011), lung (Wu et al 2012), ovarian (Qiu et al 2013), and osteosarcoma (Gu et al 2014). HJURP overexpression was first identified in lung (Kato et al 2007) and further observed in breast (Hu et al 2010;Montes de Oca et al 2015), glioma (de Tayrac et al 2013), and astrocytoma .…”

The role of the Mis18α-β complex and its interactions with HJURP and CENP-A in human centromeric chromatin establishment

“…This implies that the suggested height difference in the experiments may be in fact due to the experimental method of atomic force microscopy that may not be biologically relevant 36 . Evidence for an octameric CENP-A nucleosome has been demonstrated showing the dimerization interface between CENP-A/Cse4 is required for stable deposition at centromeres in budding yeast, flies, and humans [43][44][45] .…”