Expression and promoter analysis of the TaLTP1 gene induced by drought and salt stress in wheat (Triticum aestivum L.)

Jang, Cheol Seong; Lee, Ho Joung; Chang, Suck Joo; Seo, Yong Weon

doi:10.1016/j.plantsci.2004.05.019

Cited by 73 publications

(43 citation statements)

References 27 publications

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“…Transcript accumulation of only a few Triticum aestivum nsLtp genes has been monitored during grain development (Altenbach and Kothari 2004;Boutrot et al 2005), in crown of wheat subjected to cold (Gaudet et al 2003) or fungal infection (Lu et al 2005), and in plants of a wheat-rye translocation line exposed to diVerent biotic and abiotic stress (Jang et al 2004(Jang et al , 2005.…”

Section: Introductionmentioning

confidence: 99%

The Triticum aestivum non-specific lipid transfer protein (TaLtp) gene family: comparative promoter activity of six TaLtp genes in transgenic rice

Boutrot

Meynard²,

Guiderdoni³

et al. 2006

Planta

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Plant non-specific lipid transfer proteins (nsLTPs) are encoded by a multigene family and support physiological functions, which remain unclear. We adapted an efficient ligation-mediated polymerase chain reaction (LM-PCR) procedure that enabled isolation of 22 novel Triticum aestivum nsLtp (TaLtp) genes encoding types 1 and 2 nsLTPs. A phylogenetic tree clustered the wheat nsLTPs into ten subfamilies comprising 1-7 members. We also studied the activity of four type 1 and two type 2 TaLtp gene promoters in transgenic rice using the 1-Glucuronidase reporter gene. The activities of the six promoters displayed both overlapping and distinct features in rice. In vegetative organs, these promoters were active in leaves and root vascular tissues while no beta-Glucuronidase (GUS) activity was detected in stems. In flowers, the GUS activity driven by the TaLtp7.2a, TaLtp9.1a, TaLtp9.2d, and TaLtp9.3e gene promoters was associated with vascular tissues in glumes and in the extremities of anther filaments whereas only the TaLtp9.4a gene promoter was active in anther epidermal cells. In developing grains, GUS activity and GUS immunolocalization data evidenced complex patterns of activity of the TaLtp7.1a, TaLtp9.2d, and TaLtp9.4a gene promoters in embryo scutellum and in the grain epicarp cell layer. In contrast, GUS activity driven by TaLtp7.2a, TaLtp9.1a, and TaLtp9.3e promoters was restricted to the vascular bundle of the embryo scutellum. This diversity of TaLtp gene promoter activity supports the hypothesis that the encoded TaLTPs possess distinct functions in planta.

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Section: Introductionmentioning

confidence: 99%

The Triticum aestivum non-specific lipid transfer protein (TaLtp) gene family: comparative promoter activity of six TaLtp genes in transgenic rice

Boutrot

Meynard²,

Guiderdoni³

et al. 2006

Planta

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“…LTPs are induced by pathogens (Molina and García-Olmedo, 1993;Guiderdoni et al, 2002;Park et al, 2002), display antimicrobial activity (Nielsen et al, 1996;Molina and Garcia-Olmedo, 1997;Kristensen et al, 2000), and are involved in systemic resistance signaling (Maldonado et al, 2002). Furthermore, many LTP genes are induced by drought stress and/or water deficit (ColmeneroFlores et al, 1997;Jang et al, 2004), and some are up-regulated when exposed to both pathogens and dehydration stress (Jung et al, 2003). Several studies have confirmed that LTPs are secreted and that many members of this large family are predominately expressed in the epidermis (Sterk et al, 1991;Fleming et al, 1992;Thoma et al, 1993;Pyee et al, 1994;Canevascini et al, 1996;Smart et al, 2000).…”

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confidence: 99%

Increased Accumulation of Cuticular Wax and Expression of Lipid Transfer Protein in Response to Periodic Drying Events in Leaves of Tree Tobacco

2005

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Cuticular wax deposition and composition affects drought tolerance and yield in plants. We examined the relationship between wax and dehydration stress by characterizing the leaf cuticular wax of tree tobacco (Nicotiana glauca L. Graham) grown under periodic dehydration stress. Total leaf cuticular wax load increased after each of three periods of dehydration stress using a CH 2 Cl 2 extraction process. Overall, total wax load increased 1.5-to 2.5-fold, but composition of the wax was not altered. Homologous series of wax components were classified into organic groups; n-hentriacontane was the largest component (.75%) with alcohols and fatty acids representing ,10% of the entire wax load. An increase in density, but no change in the three-dimensional shape, of leaf wax crystals was evident under low-kV scanning electron microscopy after each drying event. Leaves excised from plants subjected to multiple drying events were more resistant to water loss compared to leaves excised from well-watered plants, indicating that there is a negative relationship between total wax load and epidermal conductance. Lipid transfer proteins (LTPs) are thought to be involved in the transfer of lipids through the extracellular matrix for the formation of cuticular wax. Using northern analysis, a 6-fold increase of tree tobacco LTP gene transcripts was observed after three drying events, providing further evidence that LTP is involved in cuticle deposition. The simplicity of wax composition and the dramatic wax bloom displayed by tree tobacco make this an excellent species in which to study the relationship between leaf wax deposition and drought tolerance.

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“…TaLTP1 gene encodes plant lipid transfer proteins (LTPs), which has a role in in vitro transfer of phospholipids and facilitates the formation and reinforcement of plant surface layers, embryogenesis and defence against pathogens, symbiosis and abiotic stress tolerance (Kader 1996). Transcripts of the TaLTP1 are increased by drought, salinity, wounding and application of SA, ethephon and H 2 O 2 (Jang et al 2004). OsGGT (asubmergence-induced gene) is determined in submergence-tolerant cultivar of rice (Oryza sativa L.) (Qi et al 2005).…”

Section: Salicylic Acid-induced Gene Expression and Its Implications mentioning

confidence: 99%

Salicylic Acid and Drought Stress Response: Biochemical to Molecular Crosstalk

Pandey

Chakraborty

2015

Stress Responses in Plants

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Salicylic acid, a naturally occurring phenolic compound, is a multifaceted plant growth modulator and activates the systemic acquired defence in plants as a response to pathogen effect. In recent years in addition to the activation of SAR, SA is reported to play a major role in the modulation of plant responses to biotic and abiotic stresses like drought, salinity, heat, heavy metal stress, osmotic stress, defence against pathogenic elicitors and effectors and symbiotic relationships. Additionally, SA has well laid out physiological roles in growth and development of plants. Several of the targets of SA have been recognized, and the molecular mode of action elucidating the complex signal transduction and involving crosstalk of multiple metabolic pathways is being unravelled. This chapter deals with recent findings on the improvement of drought tolerance vis-a-vis salicylic acid-induced modulation of metabolic pathways and signalling mechanisms.

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Expression and promoter analysis of the TaLTP1 gene induced by drought and salt stress in wheat (Triticum aestivum L.)

Cited by 73 publications

References 27 publications

The Triticum aestivum non-specific lipid transfer protein (TaLtp) gene family: comparative promoter activity of six TaLtp genes in transgenic rice

The Triticum aestivum non-specific lipid transfer protein (TaLtp) gene family: comparative promoter activity of six TaLtp genes in transgenic rice

Increased Accumulation of Cuticular Wax and Expression of Lipid Transfer Protein in Response to Periodic Drying Events in Leaves of Tree Tobacco

Salicylic Acid and Drought Stress Response: Biochemical to Molecular Crosstalk

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