Expression and Purification of Recombinant Immunogenic Proteins of Goat Poxvirus in Prokaryotic System

Kumar, Amit; Venkatesan, Gnanavel; Kushwaha, Anand; Kumar, Pavan; Ramakrishnan, Muthannan Andavar; Dhar, P.

doi:10.20546/ijcmas.2019.801.208

Cited by 4 publications

(2 citation statements)

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“…As employed in this study, the P32 has successfully been used for explicit detection and differentiation of goat pox virus [ 10 , 27 – 29 ]. The P32 gene obtained from the samples showed similar resemblance to reference Capripox strains and other goat pox strains [ 12 , 14 , 26 , 30 , 31 ]. Likewise, the nucleotide analysis also yielded a similarity score to other goatpox virus strains [ 10 , 27 ].…”

Section: Discussionmentioning

confidence: 65%

“…Furthermore, a high specificity and sensitivity by PCR confirmation of P32 recombinant plasmid cloned into Pichia host GSII5 compared to serum neutralization test or indirect ELISA has been recorded [ 30 ]. Cloning of F33L and truncated P32 genes by Kumar et al [ 31 ] reported that the purified recombinant was potentially useful for serodiagnosis of capripoxviruses. Utilization of A32L gene from field isolated goat pox virus strain in Taiwan [ 12 ] suggested that multiplex PCR and high resolution melt analysis (HRMA) could play and important role in goat pox virus diagnosis.…”

Section: Discussionmentioning

confidence: 99%

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Molecular phylogenetics of a recently isolated goat pox virus from Vietnam

et al. 2021

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Background After a decade of silence, an outbreak of the contagious and Asian endemic disease, goat pox re-emerged in North Vietnam affecting more than 1800 heads with a mortality rate of 6.5%. The inevitable impact of goat pox on hide quality, breeding, chevon and milk production has resulted in a significant economic losses to the developing goat industry of Vietnam. In the act of establishing an effective control of this devastating disease, tracing the source of re-emergence via a phylogenetic study was carried out to reveal their genetic relatedness. Either skin scab or papule from the six affected provinces were collected, cultured into Vero cells followed by restricted enzyme digestion of targeted P32 gene DNA encoding. The P32 gene was then cloned and transformed into E.coli competent cells for further sequencing. Results The isolated sequence is deposited into GenBank under Accession No. MN317561/VNUAGTP1. The phylogenetic tree revealed high similarity of nucleotide and amino acid sequences to references goat pox strains accounting for 99.6 and 99.3, respectively. The Vietnamese strain is clustered together with currently circulating goat pox virus in China, India and Pakistan which suggested the origin of South China. Conclusions This Vietnam isolate is clustered together with other Asian goat pox strains indicating the dissemination of a common goat pox virus within this continent.

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