Expression and regulation of cholesterol sulfotransferase (SULT2B1b) in human endometrium

Expression of retinoic acid-related orphan receptor alpha and its responsive genes in human endometrium regulated by cholesterol sulfate

“…We previously reported that CS increased during the implantation period in rabbit endometrium [1,3]. Also, in human endometrium, we recently revealed that cholesterol sulfotransferase 2B1b, an enzyme of CS synthesis, increased during the mid-secretory phase of the menstrual cycle [4]. In the present study, the amount of RORA mRNA increased during the mid-secretory phase (Fig.…”

“…The techniques used for isolation and culture of human endometrial stromal cells (ESCs) and epithelial cells (EECs) were previously described in the literature [4,27,28]. Fresh endometrial biopsy specimens collected in a sterile medium were rinsed to remove blood cells.…”

“…Specifically, the level of acidic glycosphingolipids dramatically increases during the secretory phase [1][2][3], whereas the level of cholesterol sulfate (CS) increases in rabbit endometrium during the implantation window and steroid sulfatase activity concomitantly decreases [1,3]. In human endometrium, cholesterol sulfotransferase 2B1b (SULT2B1b), an enzyme involved in CS synthesis, is increased in human endometrium during the implantation period [4]. CS is a major sterol sulfate in human plasma that is detectable in the endometrial cells of the uterus, lungs, skin, hair, adrenal glands, and nails.…”

Inhibition of cell invasion and protease activity by cholesterol sulfate

“…In the present study, we demonstrated the inhibitory effect of CS with a physiologic concentration on cell invasion by a double-filter assay. Our previous data showed increase in CS and SULT2B1 levels in rabbit and human endometrium during the implantation period (1-3), and CS secretion from endometrial cells (3). Considering these results, CS may act as a signaling molecule between the trophoblast and endometrium, and may regulate the implantation process.…”

“…In 1991, Momoeda et al (1,2) reported that the characteristic accumulation of cholesterol sulfate (CS) in rabbit endometrium during the implantation period is followed by a gradual decline. Our recent study revealed that the level of the cholesterol sulfotransferase SULT 2B1b, an enzyme involved in CS synthesis, increased in the human endometrium during the midsecretory phase of the menstrual cycle, which is considered the implantation period in humans (3). Cholesterol sulfate is a major sterol sulfate in human plasma, and is detectable in the uterine endometrium, lung, skin, hair, adrenal gland, and nails.…”