Expression and regulation of Runx3 in mouse uterus during the peri-implantation period

Bai, Zhikun; Guo, Bin; Tian, Xiaochen; Li, Dangdang; Wang, Shoutang; Cao, Huiming; Wang, Qu-Yuan; Yue, Zhan‐Peng

doi:10.1007/s10735-013-9501-z

Cited by 7 publications

(2 citation statements)

References 20 publications

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“…MMP-2 and MMP-9 are enriched in the invadopodia where they contribute to ECM degradation in vitro and in vivo (104). In our previous studies, we provided evidence that RUNX3 can suppress MMP-2 activities in prostate and breast cancers, but MMP-9 in renal cell carcinoma (18,24,105). We suppose that RUNX3 regulate cell invasion through different MMPs depending on the cancer tissue.…”

Section: Runx3 In Angiogenesismentioning

confidence: 83%

The emerging role of RUNX3 in cancer metastasis (Review)

et al. 2015

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Metastasis remains the major driver of mortality in patients with cancer. The multistep metastatic process starts with the dissemination of tumor cells from a primary site and leading to secondary tumor development in an anatomically distant location. Although significant progress has been made in understanding the molecular characteristics of metastasis, many questions remain regarding the intracellular mechanisms governing transition through the various metastatic stages. The runt-related transcription factor 3 (RUNX3) is a downstream effector of the transforming growth factor-β (TGF-β) signaling pathway, and has critical roles in the regulation of cell death by apoptosis, and in angiogenesis, epithelial-to-mesenchymal transition (EMT), cell migration and invasion. RUNX3 functions as a bona fide initiator of carcinogenesis by linking the Wnt oncogenic and TGF-β tumor suppressive pathways. RUNX3 is frequently inactivated in human cancer cell lines and cancer samples by hemizygous deletion of the Runx3 gene, hypermethylation of the Runx3 promoter, or cytoplasmic sequestration of RUNX3 protein. Inactivation of RUNX3 makes it a putative tumor suppressor in human neoplasia. In the present review, we summarize the proposed roles of RUNX3 in metastasis and, when applicable, highlight the mechanism by which they function.

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Section: Runx3 In Angiogenesismentioning

confidence: 83%

The emerging role of RUNX3 in cancer metastasis (Review)

et al. 2015

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“…Moreover, although conventional 2D cell culture systems are attractive in vitro platforms for biologists and clinical users due to their simplicity and efficiency [9], most of these models have not properly recapitulate the multicellular 3D structure of human tissues. For example, the human endometrium consists of multiple cell types, such as stromal cells, vascular epithelial cells, immune cells, and endometrial stem cells [10].…”

Section: Introductionmentioning

confidence: 99%

Development of a novel dual reproductive organ on a chip: recapitulating bidirectional endocrine crosstalk between the uterine endometrium and the ovary

Park¹,

Kim²,

Lee³

et al. 2020

Biofabrication

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Conventional 2D or even 3D in vitro culture models for human reproductive organs cannot properly recapitulate the bidirectional endocrine crosstalk between the uterine endometrium and the ovary. This crosstalk is essential for maintaining the various physiological features and functions of each tissue. Moreover, most in vitro models for the female reproductive tract also fail to mimic its multicellular structure. We therefore developed a novel ‘dual reproductive organ on a chip’ that reflects the bidirectional endocrine cross-talk and the complex multicellular structures by integrating various cellular components of both the human uterine endometrium and the ovary with several biodegradable natural polymers. Indeed, the bidirectional endocrine crosstalk between these two tissues is achieved through media sharing between channels, and it can markedly improve the viability of loaded cells within each chamber of the chip platform. In addition, we also identified a reliable reproductive toxicity marker, SERPINB2, which is significantly increased in response to various toxic exposures in both endometrial and ovarian follicular cells. Based on these findings, we next established a SERPINB2 luciferase reporter system that was specifically designed for detecting and quantifying the toxicity of certain substances. By introducing this SERPINB2 luciferase reporter system into the loaded cells within the chip platform, we ultimately developed an effective ‘dual reproductive organ-on-chip’ that was successfully used to predict the reproductive toxicity of various hazardous materials.

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Differential expression and regulation of Runx1 in mouse uterus during the peri-implantation period

Bai

Guo

et al. 2015

Cell Tissue Res

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Runx1 transcription factor is a key developmental regulator. However, little is known about the effects of Runx1 on embryo implantation and decidualization. The aim of this study is to examine the expression and regulation of Runx1 in mouse uterus during the peri-implantation period. There was no evident Runx1 mRNA signal on days 1-4 of pregnancy. On day 5 of pregnancy, Runx1 mRNA was mainly localized in the subluminal stroma surrounding the implanting blastocyst. A similar result was observed in the estrogen-activated implantation uterus. Simultaneously, a high level of Runx1 mRNA expression was detected on days 6-8 of pregnancy and under artificial decidualization. 8-Br-cAMP could induce the expression of Runx1 mRNA in the uterine stromal cells. Moreover, the induction was obviously blocked by PKA inhibitor H89. Inhibition of Runx1 with specific siRNA could decrease the proliferation of stromal cells and expression of decidual markers Prl8a2 and Prl3c1 in the uterine stromal cells. Further study found that inhibition of Runx1 could also suppress the expression of Cox-2, mPGES-1 and Mmp2 genes in uterine stromal cells. Estrogen and progesterone could induce the expression of Runx1 mRNA in ovariectomized mouse uterus and uterine stromal cells. Taken together, these data suggest that Runx1 may play an important role during mouse decidualization.

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Expression and regulation of Runx3 in mouse uterus during the peri-implantation period

Cited by 7 publications

References 20 publications

The emerging role of RUNX3 in cancer metastasis (Review)

The emerging role of RUNX3 in cancer metastasis (Review)

Development of a novel dual reproductive organ on a chip: recapitulating bidirectional endocrine crosstalk between the uterine endometrium and the ovary

Differential expression and regulation of Runx1 in mouse uterus during the peri-implantation period

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