Expression and significance of CD44s, CD44v6, and nm23 mRNA in human cancer

Liu, Yongjun; Pei-song, Yan; Li, Jun; Jia, Jing-Fen

doi:10.3748/wjg.v11.i42.6601

Cited by 69 publications

(57 citation statements)

References 15 publications

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“…A significant heterogeneity was observed among these included studies in this meta-analysis, and through the subgroup analysis, we can see the heterogeneity may be produced by the cut-off scores, ethnicity, year of publication and follow-up period. We also noticed that some studies had examined CD44v6 expression in NSCLC patients using methods other than immunohistochemistry (Miyoshi et al, 1997;Lee et al, 2005;Liu et al, 2005). Using reverse transcription PCR, Lee found that CD44v6 low expression was correlated to short disease-free interval and overall survival in NSCLC (Lee et al, 2005), this result was conflict with our meta-analysis.…”

Section: Discussioncontrasting

confidence: 60%

Prognostic Value of CD44 Variant exon 6 Expression in Non-Small Cell Lung Cancer: a Meta-analysis

He¹,

Qiu²,

Chen³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Section: Discussioncontrasting

confidence: 60%

Prognostic Value of CD44 Variant exon 6 Expression in Non-Small Cell Lung Cancer: a Meta-analysis

He¹,

Qiu²,

Chen³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…51,52 At variance with other reports, in the present series, no significant relationship was found with histotypes of GC.…”

Section: Cd44v6 In Gastric Cancercontrasting

confidence: 57%

De novo expression of CD44 variants in sporadic and hereditary gastric cancer

Cunha

Oliveira

Wen

et al. 2010

Laboratory Investigation

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CD44 is the major ubiquitously expressed cell surface receptor for hyaluronate. The CD44 gene encodes several protein isoforms due to extensive alternative splicing and post-translational modifications. Some of these CD44 variable isoforms have been foreseen as key players in malignant transformation and their expression is highly restricted and highly specific, unlike the canonical CD44 standard isoform. In this study, we aimed at dissecting the mRNA splicing pattern of CD44 in normal stomach and gastric cancer (GC) cell lines (n ¼ 9) using cloning and quantitative mRNA amplification assays. Moreover, we assessed the RNA levels and protein expression pattern of relevant splicing forms in distinct premalignant and malignant gastric lesions (sporadic (n ¼ 43) and hereditary (n ¼ 3) forms) using real-time RT-PCR and immunohistochemistry. We also explored the association of CD44 and E-cadherin expression by immunohistochemistry, as E-cadherin has a pivotal functional role in GC. We established the pattern of CD44 variant forms in normal stomach and gastric malignancy. We observed that although exon v6-containing isoforms were rarely expressed in normal gastric mucosa, they became increasingly expressed both in gastric premalignant (hyperplastic polyps, complete and incomplete intestinal metaplasia, low-and high-grade dysplasia) and malignant lesions (cell lines derived from GCs, primary sporadic GCs and hereditary diffuse GCs (HDGCs)). Moreover, we verified that whenever E-cadherin expression was absent, exon v6-containing CD44 isoforms were overexpressed. The lack of expression of CD44 isoforms containing exon v6 in the surface and foveolar epithelia of normal stomach and, its de novo expression in premalignant, as well as in sporadic and hereditary malignant lesions of the stomach, pinpoint CD44 v6-containg isoforms as potential biomarkers for early transformation of the gastric mucosa. Further, our results raise the hypothesis of using CD44v6 as a marker of early invasive intramucosal carcinoma in HDGC CDH1 mutation carriers that lack CDH1 expression in their tumors.

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“…This finding suggests that this protein plays a role in the chain of events involved in neoplastic changes. The NM23 gene codifies subunits of nucleoside diphosphate kinase protein involved in cell differentiation (7,24,28) . NM23 expression is therefore expected to be increased in processes of cell multiplication.…”

Section: Discussionmentioning

confidence: 99%

NM23 protein expression in colorectal carcinoma using TMA (tissue microarray): association with metastases and survival

Oliveira

Neto

Waisberg

et al. 2010

Arq. Gastroenterol.

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-Context -NM23, a metastasis suppressor gene, may be associated with prognosis in patients with colorectal carcinoma.Objective -To analyze NM23 expression and its association with the presence of lymph node and liver metastases and survival in patients operated on for colorectal carcinoma. Methods -One hundred thirty patients operated on for colorectal carcinoma were investigated. Tissue microarray blocks containing neoplastic tissue and tumor-adjacent non-neoplastic mucosa were obtained and analyzed by immunohistochemical staining using a monoclonal anti-NM23 antibody. Immunohistochemical expression was assessed using a semiquantitative scoring method, counting the percentage of stained cells. The results were compared regarding morphological and histological characteristics of the colorectal carcinoma, presence of lymph node and liver metastases, tumor staging, and patient survival. Statistical analysis was performed using the Mann-Whitney test, the Kruskal-Wallis test and Fisher's exact test. Survival analysis was performed using the Kaplan-Meier method and the log-rank test. Results -NM23 expression was higher in colorectal carcinoma tissue than in adjacent non-neoplastic mucosa (P<0.0001). NM23 protein expression did not correlate with degree of cell differentiation (P = 0.57), vascular invasion (P = 0.85), lymphatic invasion (P = 0.41), perineural infiltration (P = 0.46), staging (P = 0.19), lymph node metastases (P = 0.08), or liver metastases (P = 0.59). Disease-free survival showed significant association (P = 0.01) with the intensity of NM23 protein immunohistochemical expression in colorectal carcinoma tissue, whereas overall survival showed no association with NM23 protein expression (P = 0.13). Conclusions -NM23 protein expression was higher in neoplastic colorectal carcinoma tissue than in adjacent non-neoplastic mucosa, showing no correlation with morphological aspects, presence of lymph node or liver metastases, colorectal carcinoma staging, or overall survival. Disease-free survival was higher in patients with increased NM23 expression.

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Expression and significance of CD44s, CD44v6, and nm23 mRNA in human cancer

Cited by 69 publications

References 15 publications

Prognostic Value of CD44 Variant exon 6 Expression in Non-Small Cell Lung Cancer: a Meta-analysis

Prognostic Value of CD44 Variant exon 6 Expression in Non-Small Cell Lung Cancer: a Meta-analysis

De novo expression of CD44 variants in sporadic and hereditary gastric cancer

NM23 protein expression in colorectal carcinoma using TMA (tissue microarray): association with metastases and survival

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