Expression and significance of IGF-2, PCNA, MMP-7, and ?-actin in gastric carcinoma with Lauren classification

Kuang, Rong-Guang; Wu, Heng-Xiang; Hao, Guo-Xiang; Wang, Jianwen; Zhou, Chengjun

doi:10.4318/tjg.2013.0571

Cited by 16 publications

(8 citation statements)

References 32 publications

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“…PCNA plays a pivotal role in numerous cellular processes, for instance DNA replication, cell cycle progression, transcription, and chromosome segregation [ 29 , 30 , 31 ]. Consequently, it has been previously reported that the expression level of PCNA was higher in cancer cells and cancer tissues, such as breast, gastric, and lung cancer [ 32 , 33 , 34 , 35 ]. It has been found that novel classes of the compound known as PCNA inhibitor (PCNA-I1) can directly bind to PCNA, stabilize PCNA trimers, decrease PCNA–chromatin association, and inhibit tumor cell growth by inducing a cell cycle arrest [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

Proteomic Investigation to Identify Anticancer Targets of Nemopilema nomurai Jellyfish Venom in Human Hepatocarcinoma HepG2 Cells

Choudhary

Lee

Pyo

et al. 2018

Toxins

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Nemopilema nomurai is a giant jellyfish that blooms in East Asian seas. Recently, N. nomurai venom (NnV) was characterized from a toxicological and pharmacological point of view. A mild dose of NnV inhibits the growth of various kinds of cancer cells, mainly hepatic cancer cells. The present study aims to identify the potential therapeutic targets and mechanism of NnV in the growth inhibition of cancer cells. Human hepatocellular carcinoma (HepG2) cells were treated with NnV, and its proteome was analyzed using two-dimensional gel electrophoresis, followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI/TOF/MS). The quantity of twenty four proteins in NnV-treated HepG2 cells varied compared to non-treated control cells. Among them, the amounts of fourteen proteins decreased and ten proteins showed elevated levels. We also found that the amounts of several cancer biomarkers and oncoproteins, which usually increase in various types of cancer cells, decreased after NnV treatment. The representative proteins included proliferating cell nuclear antigen (PCNA), glucose-regulated protein 78 (GRP78), glucose-6-phosphate dehydrogenase (G6PD), elongation factor 1γ (EF1γ), nucleolar and spindle-associated protein (NuSAP), and activator of 90 kDa heat shock protein ATPase homolog 1 (AHSA1). Western blotting also confirmed altered levels of PCNA, GRP78, and G6PD in NnV-treated HepG2 cells. In summary, the proteomic approach explains the mode of action of NnV as an anticancer agent. Further characterization of NnV may help to unveil novel therapeutic agents in cancer treatment.

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Section: Resultsmentioning

confidence: 99%

Proteomic Investigation to Identify Anticancer Targets of Nemopilema nomurai Jellyfish Venom in Human Hepatocarcinoma HepG2 Cells

Choudhary

Lee

Pyo

et al. 2018

Toxins

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“…Evidence is accumulating that patient prognosis and treatment response depend not only on the tumor stage but also on tumor-specific alterations of both gene expression and various signaling pathways. The two major histological subtypes of GC according to Laurén, diffuse-type and intestinal-type GC, have distinct tumor dissemination patterns and show diverse pathogeneses and expression profiles, likely resulting from molecular differences in tumor epithelial and stroma cells [ 4 , 5 ]. Although the distinction between diffuse and intestinal subtype in GC has prognostic significance, it is still widely neglected in patient-tailored treatment of GC [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Integrins αvβ3 and αvβ5 as prognostic, diagnostic, and therapeutic targets in gastric cancer

et al. 2014

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BackgroundWe investigated the expression of two αv integrins, αvβ3 and αvβ5, in gastric cancer (GC) by testing the following hypotheses: that these molecules are expressed in GC; that they are implicated in GC biology; that they help to distinguish between the two major histological subtypes of GC, according to Laurén; and that they are prognostically relevant.MethodsFormalin-fixed and paraffin-embedded tissue samples from 482 GC samples were stained immunohistochemically using rabbit monoclonal antibodies directed against αvβ3 (EM22703) and αvβ5 (EM09902). Immunostaining of tumor, stroma, and endothelial cells was evaluated separately by the quantity and intensity, generating an immunoreactivity score. The immunoreactivity score of both antibodies was correlated with clinicopathology data and patient survival.ResultsEach integrin was expressed in at least one tumor component in all GCs. Both were expressed significantly more often in the intestinal phenotype according to Laurén. Moreover, patients who grouped as “positive” for expression of αvβ3 on endothelial cells, and patients with an intestinal type GC, grouped as “negative” for expression of αvβ5 on stroma cells, had significantly longer survival. The expression of αvβ5 on stroma cells was confirmed to be an independent prognostic factor of intestinal-type GC.ConclusionThe expression of αvβ3 and αvβ5 in at least one tumor component in all GC samples is an interesting new result that should form a basis for further investigations; for example, regarding selective integrin antagonists and the value of αvβ3 and αvβ5 as putative prognostic biomarkers. Moreover, both markers might be helpful in the routine classification of GC subtypes.Electronic supplementary materialThe online version of this article (doi:10.1007/s10120-014-0435-2) contains supplementary material, which is available to authorized users.

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“…breast cancer [29], gastric cancer [30], prostate cancer [31], and NSCLC [32]. In our study, MMP9 showed distinctive biological activity levels in different subtypes of lung adenocarcinoma.…”

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confidence: 49%

“…Expression of genes involved in tumor cell invasion and metastasis were upregulated between 10‐fold (MMP2) and 5000‐fold (MMP9), increased gene expression of MMP9, and decreased gene expression of tumor suppressor p53 was associated with poor tumor differentiation . Moreover, the level of MMP9 expression was also correlated with cancer classification and overall survival of breast cancer , gastric cancer , prostate cancer , and NSCLC . In our study, MMP9 showed distinctive biological activity levels in different subtypes of lung adenocarcinoma.…”

Section: Discussionmentioning

confidence: 99%

Prognostic value of MMP9 activity level in resected stage I B lung adenocarcinoma

et al. 2016

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The clinical outcomes of patients with early‐stage non‐small cell lung cancer (NSCLC) have remained unsatisfactory after complete surgical resection. The objective of this study was to explore the prognostic value of matrix metalloproteinase 9 (MMP9) activity level in Chinese patients with stage I B lung adenocarcinoma. A sensitive and validated method was employed for determining the activity of MMP9 in human lung adenocarcinoma cells in vitro. Then, the association was examined between the level of MMP9 enzymatic activity and clinical outcomes. A total of 104 cases were stratified according to the IASLC/ATS/ERS classification scheme and activity of MMP9 was analyzed by SensoLyte® assay kit. The results showed that the MMP9 activity was the highest in solid predominant and micropapillary predominant subtypes, intermediate in acinar predominant and papillary predominant subtypes, and the lowest in lepidic predominant subtype. Multivariate analysis revealed that pathological subtype and activity of MMP9 were independent prognostic factors for disease‐free survival (DFS), respectively (P = 0.005 and 0.029). Significant relationship existed between enzyme activity of MMP9 and prognosis. And the 30 months DFS of high‐ and low‐level MMP9 activity tumors was 44.2% and 84.1% (P < 0.0001), respectively. High‐level MMP9 activity is correlated with aggressive tumor behaviors and poor clinical outcomes in early‐stage lung adenocarcinoma after complete resection.

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Expression and significance of IGF-2, PCNA, MMP-7, and ?-actin in gastric carcinoma with Lauren classification

Cited by 16 publications

References 32 publications

Proteomic Investigation to Identify Anticancer Targets of Nemopilema nomurai Jellyfish Venom in Human Hepatocarcinoma HepG2 Cells

Proteomic Investigation to Identify Anticancer Targets of Nemopilema nomurai Jellyfish Venom in Human Hepatocarcinoma HepG2 Cells

Integrins αvβ3 and αvβ5 as prognostic, diagnostic, and therapeutic targets in gastric cancer

Prognostic value of MMP9 activity level in resected stage I B lung adenocarcinoma

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