Expression and significance of MOC‐31 and calretinin in pleural fluid of patients with lung cancer

Abstract

MOC-31 and calretinin are of important clinical value for diagnosing and differentially diagnosing the cancer cells in pleural fluid of patients with lung cancer.

The importance of EZH2 and MOC‐31 expression in the differential diagnosis of benign and malignant effusions

“…6,12,16,25,29 In the current study, all serous effusions were found to be negative for MOC-31. 6,12,16,25,29 In the current study, all serous effusions were found to be negative for MOC-31.…”

“…The presence of tumor cells in serous fluids reflects poor prognosis. 6,7,12 In many studies, MOC-31 has been used for the differentiation of reactive mesothelial proliferation or mesothelioma from metastatic adenocarcinoma in serous effusions. In mesothelial proliferation, there are reactive changes due to damage on the serosal surfaces, and mesothelioma overlap with the cytological characteristics of metastatic malignant epithelial tumors (particularly of adenocarcinomas), and therefore, differential diagnosis is difficult with smear and conventional stains only.…”

“…In mesothelial proliferation, there are reactive changes due to damage on the serosal surfaces, and mesothelioma overlap with the cytological characteristics of metastatic malignant epithelial tumors (particularly of adenocarcinomas), and therefore, differential diagnosis is difficult with smear and conventional stains only. 6,7,[13][14][15] In a meta-analysis of 18 publications between 1994 and 2012, Li et al 29 reported that MOC-31 had sensitivity and specificity of 85 and 97% in malignant effusions, respectively. [1][2][3][4][5] In the current series, there was only one case of mesothelioma, negative for MOC and positive for EZH2.…”

“…[1][2][3] Mesothelial cells paving the serosal surface have ovalround paracentric nuclei with smooth contours and small nucleoli. [1][2][3][4][5][6] The differential diagnosis of epithelial tumor metastasis (particularly adenocarcinoma) of pleural effusions from mesothelioma and reactive mesothelial cells is difficult because of cytomorphological similarities. The nuclear/cytoplasmic ratio varies.…”

“…1 For the differentiation of reactive mesothelial proliferation and mesothelioma from malignant epithelial tumor, mesothelial markers, such as calretinin, mesothelin, WT-1, cytokeratin 5/6, and D2-40 are used while epithelial markers include carcinoembryonic antigen (CEA), Ber-Ep4, B72.3, and MOC-31. 6,12 At present, it is often used as a marker to differentiate mesothelioma or reactive mesothelial proliferation from metastatic adenocarcinoma in effusions. [9][10][11][12][13][14][15][16] In literature these markers have shown variable sensitivity and specificity, and calretinin and mesothelin have also shown immunoreactivity with nonmesothelial cells.…”