Expression and Significance of the Wip1 Proto-oncogene in Colorectal Cancer

Li, Zongtao; Zhang, Liu; Gao, Xiao-Zeng; Jiang, Xiaohua

doi:10.7314/apjcp.2013.14.3.1975

Cited by 14 publications

(9 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another study reveals that in breast cancer treatment, decreased expression of PPM1D improves the effect of doxorubicin‐induced apoptosis via activating p53‐mediated signaling pathway in MCF‐7 breast cancer cell line . In addition, the predictive role of PPM1D on prognosis has been reported by several researches in solid tumors . For example, in colorectal cancer, patients with high levels of PPM1D show worse five‐year OS and recurrence‐free survival compared with those with low levels of PPM1D .…”

Section: Discussionmentioning

confidence: 94%

“…Protein phosphatase Mg2+/Mn2+ dependent 1D is reported to be a growth‐promoting phosphatase via exerting negative control on several tumor suppressor pathways and functions as an oncogene in various solid tumors . For example, clinical experiments indicate that PPM1D is highly expressed in non–small‐cell lung cancer tissues compared with normal lung tissues, and PPM1D overexpression is correlated with advanced tumor features (increased tumor size and lower histological differentiation) in NSCLC patients .…”

Section: Discussionmentioning

confidence: 99%

“…Regarding the correlation of PPM1D with prognosis, some recent studies report the positive association of PPM1D with high chemotherapy resistance and undesirable survival profile in several solid tumors . For example, one study indicates that downregulation of PPM1D activates Chk1 and p53, which further increases the ovarian cancer cell sensibility to cisplatin treatment .…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Potentiality of Protein phosphatase Mg²⁺/Mn²⁺ dependent 1D as a biomarker for predicting prognosis in acute myeloid leukemia patients

et al. 2020

Clinical Laboratory Analysis

View full text Add to dashboard Cite

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. AbstractObjective: The present study aimed to investigate the correlation of protein phosphatase Mg 2+ /Mn 2+ dependent 1D (PPM1D) with the risk stratification, treatment response, and survival profile in acute myeloid leukemia (AML) patients.Methods: Totally 221 de novo AML patients and 50 healthy donors were enrolled.The bone marrow samples were collected before treatment from AML patients and acquired after enrollment from healthy donors. And bone marrow mononuclear cells were separated for detecting the mRNA/protein expressions of PPM1D by reverse transcription-quantitative polymerase chain reaction and Western blot. Complete remission (CR) was assessed after induction treatment, and event-free survival (EFS) and overall survival (OS) were calculated in AML patients.Results: PPM1D mRNA (P < .001)/protein (P < .001) relative expressions were increased in AML patients compared with healthy donors, and receiver operating characteristic curve presented that PPM1D mRNA (AUC: 0.728, 95% CI: 0.651-0.806)/ protein (AUC: 0.782, 95% CI: 0.707-0.857) relative expressions could differentiate AML patients from healthy donors. In AML patients, PPM1D mRNA (P < .001)/protein (P < .001) high relative expressions were correlated with poor-risk stratification.As for its association with prognosis, PPM1D mRNA (P < .001)/protein (P = .010) relative expressions were elevated in CR patients compared with non-CR patients.Patients with PPM1D mRNA (P < .001 for EFS; P = .004 for OS)/protein (P < .001 for EFS; P = .006 for OS) high relative expressions exhibited reduced EFS and OS compared with those with low expressions.Conclusion: PPM1D high expression correlates with poor-risk stratification and might serve as a potential biomarker for worse prognosis in AML patients, suggesting its potential to guide AML management. K E Y W O R D S acute myeloid leukemia, complete remission, protein phosphatase Mg 2+ /Mn 2+ dependent 1D, risk stratification, survival profile S U PP O RTI N G I N FO R M ATI O N Additional supporting information may be found online in the Supporting Information section. How to cite this article: Yu M, Hu J, He D, et al. Potentiality of Protein phosphatase Mg 2+ /Mn 2+ dependent 1D as a biomarker for predicting prognosis in acute myeloid leukemia patients. J Clin Lab Anal. 2020;34:e23171. https ://doi.

show abstract

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Potentiality of Protein phosphatase Mg²⁺/Mn²⁺ dependent 1D as a biomarker for predicting prognosis in acute myeloid leukemia patients

et al. 2020

Clinical Laboratory Analysis

View full text Add to dashboard Cite

show abstract

“…Thereby, Wip1 promotes tumorigenesis (11). The Wip1 gene has been reported to be overexpressed in a variety of tumors (12,13). Previous studies reported an association between Wip1 and sensitivity towards chemotherapy.…”

Section: Introductionmentioning

confidence: 99%

Wip1 gene silencing enhances the chemosensitivity of human colon cancer cells

Xia

et al. 2017

Oncology Letters

View full text Add to dashboard Cite

Abstract. Colon cancer is one of the most common cancers in the world. Multidrug resistance is one of the main reasons for failure of therapy in patients with advanced colon cancer. In previous studies, multiple methods were investigated to reverse the multidrug resistance of colon cancer cells. However, to date, no clinical method has been identified to be satisfactory. Therefore, successful reversal of drug resistance in colon cancer cells still requires new therapeutic strategies or pharmaceuticals. Wild-type p53-induced phosphatase (Wip1), a member of the 2C type serine/threonine protein phosphatase family, is closely associated with the p53 gene, which is the most important tumor-suppressor gene. Wip1 was reported to be associated with the chemosensitivity of breast cancer cells. However, the correlation between the expression of Wip1 gene and the chemosensitivity of colon cancer cells has not been reported yet. In the present study, Wip1-811 small interfering RNA (siRNA) targeting Wip1 was investigated to reverse the multidrug resistance of colon cancer cells. The siRNA duplexes were transfected into RKO colon cancer cells. The messenger RNA (mRNA) expression of Wip1 was measured by reverse transcription-quantitative polymerase chain reaction. The protein level of Wip1 was detected by western blotting. The cell viability was measured by MTS assay. The cell apoptosis and cell cycle were analyzed by flow cytometry. Intracellular adriamycin cumulative concentration was determined using flow cytometry. Wip1-811 siRNA efficiently inhibited the expression of Wip1 at the mRNA and protein levels, and enhanced the sensitivity of RKO colon cancer cells towards chemotherapy, which was accompanied by increased cell apoptosis, following the inhibition of Wip1 gene expression. These results indicate that Wip1 gene silencing could enhance the chemosensitivity of colon cancer cells, which may provide a new potential approach for the reversal of multidrug resistance in colon cancer cells.

show abstract

“…The overexpression and amplification of Wip1 had been previously identified in numerous types of human cancer including medulloblastomas (3), clear cell renal cell carcinoma (4), colorectal cancer (5) and breast cancer (6). In addition, Bulavin et al (7) demonstrated that Wip1 functioned as a proto-oncogene in breast cancer.…”

Section: Introductionmentioning

confidence: 99%

Clinical significance of Wip1 overexpression and its association with the p38MAPK/p53/p16 pathway in NSCLC

Yang

Dong

et al. 2016

Molecular Medicine Reports

View full text Add to dashboard Cite

Wip1 is deregulated in numerous human malignancies. However, its roles in non-small cell lung cancer (NSCLC) remain unclear. In the current study, the expression of Wip1 was investigated in NSCLC and its clinical significance was detected. Immunohistochemical staining was used to measure the expression of (wild-type p53 induced phosphatase 1) Wip1, p38 mitogen-activated protein kinase (MAPK), p53, p16 protein in a group of 60 NSCLC and 20 normal lung tissues. In addition, western blotting was performed to detect the Wip1 protein in fresh tissues. The correlations between clinical characteristics and Wip1 expression were analyzed using SPSS, version 16.0 software. The expression of Wip1 was positive in 63.3% (38/60) of NSCLC tissues, and in none of the normal lung tissues (0/20; P<0.01). In addition, Wip1 overexpression was significantly associated with tumor length and differentiation (P=0.008 and 0.03, respectively). The expression of Wip1 was negatively correlated with that of p38MAPK, p53 and p16 (r=−0.284, −0.352 and −0.348, respectively). The results of the current study demonstrated that Wip1 was frequently overexpressed in NSCLC, which may serve an essential role in the p38MAPK/p53/p16 signaling pathway.

show abstract

Expression and Significance of the Wip1 Proto-oncogene in Colorectal Cancer

Cited by 14 publications

References 20 publications

Potentiality of Protein phosphatase Mg²⁺/Mn²⁺ dependent 1D as a biomarker for predicting prognosis in acute myeloid leukemia patients

Potentiality of Protein phosphatase Mg²⁺/Mn²⁺ dependent 1D as a biomarker for predicting prognosis in acute myeloid leukemia patients

Wip1 gene silencing enhances the chemosensitivity of human colon cancer cells

Clinical significance of Wip1 overexpression and its association with the p38MAPK/p53/p16 pathway in NSCLC

Contact Info

Product

Resources

About

Expression and Significance of the Wip1 Proto-oncogene in Colorectal Cancer

Cited by 14 publications

References 20 publications

Potentiality of Protein phosphatase Mg2+/Mn2+ dependent 1D as a biomarker for predicting prognosis in acute myeloid leukemia patients

Potentiality of Protein phosphatase Mg2+/Mn2+ dependent 1D as a biomarker for predicting prognosis in acute myeloid leukemia patients

Wip1 gene silencing enhances the chemosensitivity of human colon cancer cells

Clinical significance of Wip1 overexpression and its association with the p38MAPK/p53/p16 pathway in NSCLC

Contact Info

Product

Resources

About

Potentiality of Protein phosphatase Mg²⁺/Mn²⁺ dependent 1D as a biomarker for predicting prognosis in acute myeloid leukemia patients

Potentiality of Protein phosphatase Mg²⁺/Mn²⁺ dependent 1D as a biomarker for predicting prognosis in acute myeloid leukemia patients