Expression and single-nucleotide polymorphisms of the H-FABP gene in pigs

Shang, Peng; Zhang, Bo; Zhang, Jian; Duan, Mengqi; Wu, Lvcao; Gong, Xinglong; Tang, Keren; Chamba, Yangzom

doi:10.1016/j.gene.2019.05.061

Cited by 10 publications

(9 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This stems from the fact that IMF provides the needed marbling for taste, juiciness, and tenderness, while DHA being the major prevalent fatty acid in the brain membrane, is vital for the maintenance of healthy and functional brain development in infants and adults [94]. In pigs, Shang et al [95] identified 3 FABP gene SNP and demonstrated that the genotype C-1375G was associated with fat deposition, while Gao et al [96] reported that an association analysis of FABP SNP indicated that the polymorphism had a significant effect on marbling, in which pigs with the DD genotype had higher marbling than CD and CC genotypes, but the difference between CD and CC genotypes was not significant. They also reported that this FABP SNP had a highly significant effect on intramuscular fat content (p < 0.01).…”

Section: Fabp Gene Polymorphismmentioning

confidence: 99%

Next Generation Sequencing of Single Nucleotide Polymorphic DNA-Markers in Selecting for Intramuscular Fat, Fat Melting Point, Omega-3 Long-Chain Polyunsaturated Fatty Acids and Meat Eating Quality in Tattykeel Australian White MARGRA Lamb

Pewan

Otto

Huerlimann

et al. 2021

Foods

View full text Add to dashboard Cite

Meat quality data can only be obtained after slaughter when selection decisions about the live animal are already too late. Carcass estimated breeding values present major precision problems due to low accuracy, and by the time an informed decision on the genetic merit for meat quality is made, the animal is already dead. We report for the first time, a targeted next-generation sequencing (NGS) of single nucleotide polymorphisms (SNP) of lipid metabolism genes in Tattykeel Australian White (TAW) sheep of the MARGRA lamb brand, utilizing an innovative and minimally invasive muscle biopsy sampling technique for directly quantifying the genetic worth of live lambs for health-beneficial omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA), intramuscular fat (IMF), and fat melting point (FMP). NGS of stearoyl-CoA desaturase (SCD), fatty acid binding protein-4 (FABP4), and fatty acid synthase (FASN) genes identified functional SNP with unique DNA marker signatures for TAW genetics. The SCD g.23881050T>C locus was significantly associated with IMF, C22:6n-3, and C22:5n-3; FASN g.12323864A>G locus with FMP, C18:3n-3, C18:1n-9, C18:0, C16:0, MUFA, and FABP4 g.62829478A>T locus with IMF. These add new knowledge, precision, and reliability in directly making early and informed decisions on live sheep selection and breeding for health-beneficial n-3 LC-PUFA, FMP, IMF and superior meat-eating quality at the farmgate level. The findings provide evidence that significant associations exist between SNP of lipid metabolism genes and n-3 LC-PUFA, IMF, and FMP, thus underpinning potential marker-assisted selection for meat-eating quality traits in TAW lambs.

show abstract

Section: Fabp Gene Polymorphismmentioning

confidence: 99%

Next Generation Sequencing of Single Nucleotide Polymorphic DNA-Markers in Selecting for Intramuscular Fat, Fat Melting Point, Omega-3 Long-Chain Polyunsaturated Fatty Acids and Meat Eating Quality in Tattykeel Australian White MARGRA Lamb

Pewan

Otto

Huerlimann

et al. 2021

Foods

View full text Add to dashboard Cite

show abstract

“…It is noteworthy that these metabolic characteristics are consistent with slow muscle metabolism features (Hargreaves & Spriet, 2018) and in accord with muscle fiber types' conversion. In recent years, more and more studies have shown that H-FBAP (Lang et al, 2017;Shang et al, 2019;Wang et al, 2016), LPL (Wang, Zhong, et al, 2020), CPT1B , and ADIPOQ (Zhao et al, 2020) are related to the deposition of intramuscular fat, although it is still controversial whether it can promote fat deposition.…”

Section: Discussionmentioning

confidence: 99%

Effect of high‐concentrate diets on mRNA expression of genes related to muscle fiber type and metabolism of psoas major muscle in goats

Ren¹,

Li²,

Xu³

et al. 2022

Animal Science Journal

View full text Add to dashboard Cite

In the process of modern breeding, high‐concentrate diets are widely used to meet the high energy nutritional requirements of animals but change the form of access to energy and nutrients and the way the organism metabolizes them. Goat psoas major (PM) muscle is a hybrid skeletal muscle whose characteristics are important for the motility and meat quality of goats. However, there are few studies on the effects of high‐concentrate diets on the muscle type and metabolic characteristics of PM in goats. In this study, two treatment groups were set up: high concentrate group (HC) and control group (C). The expression of genes related to muscle type and metabolism of the PM was examined by quantitative PCR. The results showed that high concentrate promoted the conversion of PM fibers from intermediate to slow type at the mRNA level, improved the absorption, transport, and oxidation of fat by PM, and upregulated the expression of calpain system. These changes may be regulated by the involvement of differential expression of MSTN, Myf‐5, and IGF‐2. These results suggest that high concentrate may exert a positive effect on skeletal muscle function, metabolism, and meat quality in goats by affecting the expression of muscle type and metabolism‐related genes.

show abstract

“…Thus, researchers have considered the FABP3 gene as a candidate to regulate the IMF and tenderness of domestic animal muscle. The effect of FABP3 on IMF and meat quality is mainly due to the combination of fatty acids to preserve the concentration gradient of intracellular and extracellular fatty acids, promote fatty acids to enter the cells to increase IMF content (Atay et al 2019;Shang et al 2019) and to enhance muscle tenderness Zhou et al 2005).…”

Section: Discussionmentioning

confidence: 99%

Proteomics Analysis and Identification of Critical Proteins and Network Interactions that Regulate the Specific Deposition of IMF of Jingyuan Chicken

Huang

Zhang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Improving broiler production efficiency and delivering good quality chicken has become an exciting area of research. Many factors affect the quality of chicken, and the IMF content is one of the critical factors determining the quality of chicken. At present, there are many reports on the molecular mechanism of IMF-specific deposition in chicken; however, only a few reports discuss the specific deposition of IMF in different parts of a chicken.Methods: In order to analyze the molecular mechanism of IMF specific deposition in different parts of chickens, the present study has selected 180-days old Jingyuan chicken breast and leg muscles as the research materials, using proteomics technology and screening of PRM protein quantitative detection methods, identification and quantitative verification of proteins that control the IMF-specific deposition in the leg muscles and breast muscles of Jingyuan chickens. The protein was analyzed by advanced bioinformatics using GO, KEGG, R language, Gallus_gallus_UniPort, and other biological software, including tools and related databases for screening and identification.Results: By screening and identifying LC-MS/MS mass spectrometry data and comparing it with the Gallus_gallus_UniPort database, a total of 1940.0 proteins were screened and identified, of which 1317.0 proteins were quantified. Compared with the breast muscles, there are 190 differentially expressed proteins between leg and breast muscles. In the leg muscles, 121 proteins are up-regulated, whereas 69 are down-regulated. Gene Ontology (GO) function annotation and enrichment analysis were done for 190 differential proteins. These proteins participate in a biological process (BP), cellular component (CC), and molecular function (MF) pathways. However, a total of 286 signaling pathways involved 190 differential proteins using a KEGG pathway. Significantly different proteins between the leg and breast muscles were primarily enriched with gga00500Starchandsucrosemetabolism, gga00071 Fattyaciddegradation, gga01212Fattyacidmetabolism, gga03320PPAR signaling pathway. PRM was used to confirm and identify the differential proteins by screening quantitatively; 10 of them are related to fat deposition, namely E1BTT4, E1C0Q5 (ACAT1), F1NC38 (ACADL), Q5F420 (ACSL1), A0A1D5P3S9 (ECI1), Q5ZL56 (ACADS), F1NUQ3 (FABP3), A0A1D5PL36 (ACAA2), F1NR44 (ECHS1), Q5ZIR7 (FABP5) using STRING and Cytoscape software to analyze the network interactions of 10 differentially expressed proteins, resulting in 19 network interaction paths.Conclusion: We used modern omics technology, omics software, and related databases to screen and identify 3 key signal pathways, including 10 key functional proteins that control IMF-specific deposition in different parts of chickens. At the same time, the present results can be used for different parts of the chicken. Specifically, the molecular mechanism of IMF-specific deposition has been explained, and a key regulatory role is played by the network interaction analysis of proteins that control IMF-specific deposition in different parts of chickens. The results have a scientific value and theoretical significance for improving chicken quality and molecular breeding of high-quality local chicken breeds.

show abstract

Expression and single-nucleotide polymorphisms of the H-FABP gene in pigs

Cited by 10 publications

References 24 publications

Next Generation Sequencing of Single Nucleotide Polymorphic DNA-Markers in Selecting for Intramuscular Fat, Fat Melting Point, Omega-3 Long-Chain Polyunsaturated Fatty Acids and Meat Eating Quality in Tattykeel Australian White MARGRA Lamb

Next Generation Sequencing of Single Nucleotide Polymorphic DNA-Markers in Selecting for Intramuscular Fat, Fat Melting Point, Omega-3 Long-Chain Polyunsaturated Fatty Acids and Meat Eating Quality in Tattykeel Australian White MARGRA Lamb

Effect of high‐concentrate diets on mRNA expression of genes related to muscle fiber type and metabolism of psoas major muscle in goats

Proteomics Analysis and Identification of Critical Proteins and Network Interactions that Regulate the Specific Deposition of IMF of Jingyuan Chicken

Contact Info

Product

Resources

About