Expression and suppression of circulative aphid transmission in pea enation mosaic virus.

Demler, S. A.; Rucker-Feeney, D G; Skaf, J. S.; Zoeten, G. A. de

doi:10.1099/0022-1317-78-3-511

Cited by 41 publications

(19 citation statements)

References 35 publications

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“…Deletions in and around the homologous regions in infectious clones of BWYV also destroyed or greatly reduced accumulation of RTD in infected plants (11). In PEMV, a single, naturally occurring base change in the region homologous to the downstream readthrough element of PAV prevented readthrough and aphid transmissibility of the virus (29).…”

Section: Translationmentioning

confidence: 99%

“…They proposed that strand-switching by the replicase facilitated recombination at these sites. Furthermore, Demler et al (29) found a natural PEMV deletion mutant in which a large region of the readthrough ORF, including portions of the proximal and distal elements and all the sequence between them, was deleted. This deletion could be explained by an intramolecular strand-switching event that would be favored if the two sequence elements were located in close proximity.…”

Section: Translationmentioning

confidence: 99%

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Barley Yellow Dwarf Viruses

Miller

Rasochova²

1997

Annu. Rev. Phytopathol.

215

154

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Barley yellow dwarf viruses represent one of the most economically important and ubiquitous groups of plant viruses. This review focuses primarily on four research areas in which progress has been most rapid. These include (a) evidence supporting reclassification of BYDVs into two genera; (b) elucidation of gene function and novel mechanisms controlling gene expression; (c) initial forays into understanding the complex interactions between BYDV virions and their aphid vectors; and (d ) replication of a BYDV satellite RNA. Economic losses, symptomatology, and means of control of BYD are also discussed.

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Section: Translationmentioning

confidence: 99%

Section: Translationmentioning

confidence: 99%

Barley Yellow Dwarf Viruses

Miller

Rasochova²

1997

Annu. Rev. Phytopathol.

215

154

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show abstract

“…Genetic studies have revealed that specific viral capsid protein domains and amino acids are determinative for transmissibility and vector specificity. Examples can be found for aphid-transmitted viruses in the genera Potyvirus (1,2,11), Luteovirus (53), Polerovirus (3), Enamovirus (7), and Cucumovirus (35,36,44); for the nematode-transmitted viruses in the genus Tobravirus (25); and for fungus-transmitted viruses in the genera Tombusvirus (18,40) and Benyvirus (52). An emerging theme for a number of these viruses is that a minor capsid protein readthrough protein plays an important role in transmission; this protein is generated by a mechanism involving readthrough of the major capsid protein stop codon (reviewed in reference 4).…”

mentioning

confidence: 99%

A Conserved Capsid Protein Surface Domain of Cucumber Mosaic Virus Is Essential for Efficient Aphid Vector Transmission

Liu

Park

et al. 2002

J Virol

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A prominent feature on the surfaces of virions of Cucumber mosaic virus (CMV) is a negatively charged loop structure (the ␤H-␤I loop). Six of 8 amino acids in this capsid protein loop are highly conserved among strains of CMV and other cucumoviruses. Five of these amino acids were individually changed to alanine or lysine (an amino acid of opposite charge) to create nine mutants (the D191A, D191K, D192A, D192K, L194A, E195A, E195K, D197A, and D197K mutants). Transcripts of cDNA clones were infectious when they were mechanically inoculated onto tobacco, giving rise to symptoms of a mottle-mosaic typical of the wild-type virus (the D191A, D191K, D192A, E195A, E195K, and D197A mutants), a systemic necrosis (the D192K mutant), or an atypical chlorosis with necrotic flecking (the L194A mutant). The mutants formed virions and accumulated to wild-type levels, but eight of the nine mutants were defective in aphid vector transmission. The aspartate-to-lysine mutation at position 197 interfered with infection; the only recovered progeny (the D197K‫ء‬ mutant) harbored a second-site mutation (denoted by the asterisk) of alanine to glutamate at position 193, a proximal site in the ␤H-␤I loop. Since the disruption of charged amino acid residues in the ␤H-␤I loop reduces or eliminates vector transmissibility without grossly affecting infectivity or virion formation, we hypothesize that this sequence or structure has been conserved to facilitate aphid vector transmission.

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“…Capped in vitro run-off transcripts were synthesized from PstI-or SmaI-linearized full-length cDNA of PEMV-1 or -2, respectively (Demler et al, 1997), using T7 RNA polymerase (Ribomax; Promega). Equal amounts of PEMV-1 and -2 transcripts were mixed and approximately 1 mg of each RNA was mechanically rubbed onto the leaf surface of 10-day-old pea seedlings (Pisum sativum cv.…”

Section: Methodsmentioning

confidence: 99%

Transcriptomic analysis of intestinal genes following acquisition of pea enation mosaic virus by the pea aphid Acyrthosiphon pisum

Brault

Tanguy²,

Reinbold

et al. 2009

Journal of General Virology

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Viruses in the family Luteoviridae are strictly transmitted by aphids in a non-propagative, circulative and persistent mode. Virions ingested by aphids successively cross the gut and the accessory salivary gland epithelia before being released, together with saliva, into the plant vasculature. Virion transport through aphid cells occurs by a transcytosis mechanism. This study conducted a transcriptomic analysis of intestinal genes of the pea aphid Acyrthosiphon pisum following uptake of pea enation mosaic virus. Among the 7166 transcripts analysed, 128 were significantly regulated (105 genes downregulated and 23 upregulated). Of these genes, 5 % were involved in intracellular trafficking, endocytosis and signal transduction, three important steps in the internalization and transport of virions. The limited levels of downregulation (maximum of 3.45-fold) and upregulation (maximum of 1.37-fold) suggest that the virus hijacks a constitutive endocytosis-exocytosis mechanism without heavily perturbing cell metabolism. Although limited to about 20 % of the pea aphid genes, this work represents the first large-scale analysis of aphid gene regulation following virus acquisition. A better knowledge of this virus-vector interaction will be possible only when tools representing the complete genomic capacity of the aphid become available.

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Expression and suppression of circulative aphid transmission in pea enation mosaic virus.

Cited by 41 publications

References 35 publications

Barley Yellow Dwarf Viruses

Barley Yellow Dwarf Viruses

A Conserved Capsid Protein Surface Domain of Cucumber Mosaic Virus Is Essential for Efficient Aphid Vector Transmission

Transcriptomic analysis of intestinal genes following acquisition of pea enation mosaic virus by the pea aphid Acyrthosiphon pisum

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