Expression and tissue distribution analysis of Angiotensin II in sheep (Ovis aries) skins associated with white and black coat colors

Yin, Zhihong; Ge, Yaming; Ning, Hongmei; Zhu, Ying; Chen, Lingli; Zhang, Shouping; Xia, Xiaojing; Wang, Xinzhuang; Pang, Quanhai; Liu, Xingyou

doi:10.1016/j.acthis.2019.03.002

Cited by 3 publications

(2 citation statements)

References 30 publications

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“…Positive results were indicated by brown appearance. For negative controls, the primary antibody was replaced by non-immune bovine serum [12].…”

Section: Immunohistochemistrymentioning

confidence: 99%

Expression and tissue distribution analysis of vimentin and transthyretin proteins associated with coat colors in sheep (Ovis aries)

Yin¹,

Ma²,

Wang³

et al. 2023

Anim Biosci

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Objective: Pigment production and distribution are controlled through multiple proteins, resulting in different coat color phenotypes of sheep.Methods: The expression distribution of vimentin (VIM) and transthyretin (TTR) in white and black sheep skins was detected by liquid chromatography–electrospray ionization tandem MS (LC–ESI–MS/MS), gene ontology (GO) statistics, immunohistochemistry, Western blot, and quantitative real time polymerase chain reaction (qRT-PCR) to evaluate their role in the coat color formation of sheep.Results: LC–ESI–MS/MS results showed VIM and TTR proteins in white and black skin tissues of sheep. Meanwhile, GO functional annotation analysis suggested that VIM and TTR proteins were mainly concentrated in cellular components and biological process, respectively. Further research confirmed that VIM and TTR proteins were expressed at significantly higher levels in black sheep skins than in white sheep skins by Western blot, respectively. Immunohistochemistry notably detected VIM and TTR in hair follicle, dermal papilla, and outer root sheath of white and black sheep skins. qRT-PCR results also revealed that the expression of VIM and TTR mRNAs was higher in black sheep skins than in white sheep skins.Conclusion: The expression of VIM and TTR were higher in black sheep skins than in white sheep skins and the transcription and translation were unanimous in this study. VIM and TTR proteins were expressed in hair follicles of white and black sheep skins. These results suggested that VIM and TTR were involved in the coat color formation of sheep.

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“…Positive results were indicated by brown appearance. For negative controls, the primary antibody was replaced by non-immune bovine serum [12].…”

Section: Immunohistochemistrymentioning

confidence: 99%

Expression and tissue distribution analysis of vimentin and transthyretin proteins associated with coat colors in sheep (Ovis aries)

Yin¹,

Ma²,

Wang³

et al. 2023

Anim Biosci

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“…Table S10. Additional genetic studies on sheep coat color variation [310][311][312][313][314][315][316][317][318][319][320][321][322][323][324][325][326][327]. Table S11.…”

Section: Supplementary Informationmentioning

confidence: 99%

Genetics of the phenotypic evolution in sheep: a molecular look at diversity-driving genes

et al. 2022

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Background After domestication, the evolution of phenotypically-varied sheep breeds has generated rich biodiversity. This wide phenotypic variation arises as a result of hidden genomic changes that range from a single nucleotide to several thousands of nucleotides. Thus, it is of interest and significance to reveal and understand the genomic changes underlying the phenotypic variation of sheep breeds in order to drive selection towards economically important traits. Review Various traits contribute to the emergence of variation in sheep phenotypic characteristics, including coat color, horns, tail, wool, ears, udder, vertebrae, among others. The genes that determine most of these phenotypic traits have been investigated, which has generated knowledge regarding the genetic determinism of several agriculturally-relevant traits in sheep. In this review, we discuss the genomic knowledge that has emerged in the past few decades regarding the phenotypic traits in sheep, and our ultimate aim is to encourage its practical application in sheep breeding. In addition, in order to expand the current understanding of the sheep genome, we shed light on research gaps that require further investigation. Conclusions Although significant research efforts have been conducted in the past few decades, several aspects of the sheep genome remain unexplored. For the full utilization of the current knowledge of the sheep genome, a wide practical application is still required in order to boost sheep productive performance and contribute to the generation of improved sheep breeds. The accumulated knowledge on the sheep genome will help advance and strengthen sheep breeding programs to face future challenges in the sector, such as climate change, global human population growth, and the increasing demand for products of animal origin.

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Noninvasive assessment and classification of human skin burns using images of Caucasian and African patients

2019

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Expression and tissue distribution analysis of Angiotensin II in sheep (Ovis aries) skins associated with white and black coat colors

Cited by 3 publications

References 30 publications

Expression and tissue distribution analysis of vimentin and transthyretin proteins associated with coat colors in sheep (Ovis aries)

Expression and tissue distribution analysis of vimentin and transthyretin proteins associated with coat colors in sheep (Ovis aries)

Genetics of the phenotypic evolution in sheep: a molecular look at diversity-driving genes

Noninvasive assessment and classification of human skin burns using images of Caucasian and African patients

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