Expression changes of dopaminergic system-related genes in PC12 cells induced by manganese, silver, or copper nanoparticles

Wang, Jianyong; Rahman, M. F.; Duhart, Helen M.; Newport, Glenn D.; Patterson, Tucker A.; Murdock, Richard C.; Hussain, Saber M.; Schlager, John J.; Ali, Syed F.

doi:10.1016/j.neuro.2009.09.005

Cited by 170 publications

(128 citation statements)

References 46 publications

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“…The neurotoxicity of Ag NPs with an average size of 15 nm at a concentration of 10 μg/ml for 24 h was investigated using the dopaminergic neuronal cell line PC12 by Wang et al (2009). From the results of this study, it can be inferred that the silver is involved not only in the oxidative stress, but also in alteration of the enzymatic functions which play an important role in the dopamine depletion.…”

Section: Discussionmentioning

confidence: 92%

Antibacterial activity of thin films TiO2 doped with Ag and Cu on Gracilicutes and Firmicutes bacteria

Stoyanova¹,

Ivanova²,

Angelov³

et al. 2017

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This article aims to explore the antibacterial activity of thin films of TiO doped with Ag and Cu using two types of Gram-negative and Gram-positive test bacteria with clinical significance (Gracilicutes and Firmcutes bacteria). The thin films (thickness of about 60 nm) were deposited on glass substrates by radio frequency magnetron co-sputtering (r.f. power of 50 W) of TiO target with Ag and Cu pieces on its surface in an Ar atmosphere (0.8 Pa) without heating during the deposition. The total surface area of the Ag was 60 mm and that of the Cu was 100 mm . Bacillus cereus, Staphylococcus epidermidis, Salmonella enterica, Escherichia coli and Pseudomonas sp. were used as test strains. The antibacterial actvity of the films was evaluated by the classical Koch's method and optical density measurements. The bactericidal effect was established at different time points between 30 min and 90 min for Pseudomonas sp. and S. enterica. The Firmicutes bacteria B. cereus and S. epidermidis were killed at the 4 and 8 hour of the treatment, respectively. The effect on E. coli was bacteriostatic until the 10 hour. The results were confirmed by assessment of the bacterial dehydrogenase activity. The studied thin films of TiO co-doped with Ag and Cu have a potential for application as antibacterial coatings.

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Section: Discussionmentioning

confidence: 92%

Antibacterial activity of thin films TiO2 doped with Ag and Cu on Gracilicutes and Firmicutes bacteria

Stoyanova¹,

Ivanova²,

Angelov³

et al. 2017

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“…Several researchers have shown that ENMs agglomerate in cell culture media. 9,22 In blood, plasma proteins rapidly adsorb to nanoparticle surfaces to form a protein corona that can change the physicochemical properties and influence the fate of ENMs. 20,36,37 It is difficult to interpret some in vitro and in vivo nanoparticle studies, due to their agglomeration in cell culture media and development of the protein corona in blood.…”

Section: Discussionmentioning

confidence: 99%

“…One possibility is that the ceria ENMs were rapidly coated by proteins in the blood that changed their physicochemical properties, resulting in their rapid clearance by the mononuclear phagocyte system. 20,21 Another possibility is that the nanoparticles agglomerated very rapidly in the blood or on a biological surface, changing their distribution and cell interaction, [22][23][24][25] so that the agglomerated ceria became too large or unavailable to penetrate the intact BBB. The present study utilized a controlled system to better understand how ceria ENMs interact with brain capillary endothelial cells.…”

Section: Introductionmentioning

confidence: 99%

Brain microvascular endothelial cell association and distribution of a 5 nm ceria engineered nanomaterial

Mo¹,

Tseng²,

Wu³

et al. 2012

IJN

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Purpose: Ceria engineered nanomaterials (ENMs) have current commercial applications and both neuroprotective and toxic effects. Our hypothesis is that ceria ENMs can associate with brain capillary cells and/or cross the blood-brain barrier. Methods: An aqueous dispersion of ∼5 nm ceria ENM was synthesized and characterized in house. Its uptake space in the Sprague Dawley rat brain was determined using the in situ brain perfusion technique at 15 and 20 mL/minute flow rates; 30, 100, and 500 µg/mL ceria perfused for 120 seconds at 20 mL/minute; and 30 µg/mL perfused for 20, 60, and 120 seconds at 20 mL/ minute. The capillary depletion method and light and electron microscopy were used to determine its capillary cell and brain parenchymal association and localization. Results: The vascular space was not significantly affected by brain perfusion flow rate or ENM, demonstrating that this ceria ENM did not influence blood-brain barrier integrity. Cerium concentrations, determined by inductively coupled plasma mass spectrometry, were significantly higher in the choroid plexus than in eight brain regions in the 100 and 500 µg/mL ceria perfusion groups. Ceria uptake into the eight brain regions was similar after 120-second perfusion of 30, 100, and 500 µg ceria/mL. Ceria uptake space significantly increased in the eight brain regions and choroid plexus after 60 versus 20 seconds, and it was similar after 60 and 120 seconds. The capillary depletion method showed 99.4% ± 1.1% of the ceria ENM associated with the capillary fraction. Electron microscopy showed the ceria ENM located on the endothelial cell luminal surface. Conclusion: Ceria ENM association with brain capillary endothelial cells saturated between 20 and 60 seconds and ceria ENM brain uptake was not diffusion-mediated. During the 120-second ceria ENM perfusion, ceria ENM predominately associated with the surface of the brain capillary cells, providing the opportunity for its cell uptake or redistribution back into circulating blood.

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“…Silver nanoparticles led to dopamine depletion in PC12 cells by perturbing intracellular redox balance, and manganese and copper nanoparticles affected the expression of enzymes involved in dopamine metabolism. 271,272 PC12, a dopaminergic neuronal cell line, has been used as a cell model for in vitro neurotoxicity studies. Treatment of PC12 cells with SiO 2 nanoparticles caused a decrease in dopamine levels due to the downregulation of dopamine synthesis.…”

Section: Nanotoxicity To the Cnsmentioning

confidence: 99%

Perturbation of physiological systems by nanoparticles

et al. 2014

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Nanotechnology is having a tremendous impact on our society. However, societal concerns about human safety under nanoparticle exposure may derail the broad application of this promising technology. Nanoparticles may enter the human body via various routes, including respiratory pathways, the digestive tract, skin contact, intravenous injection, and implantation. After absorption, nanoparticles are carried to distal organs by the bloodstream and the lymphatic system. During this process, they interact with biological molecules and perturb physiological systems. Although some ingested or absorbed nanoparticles are eliminated, others remain in the body for a long time. The human body is composed of multiple systems that work together to maintain physiological homeostasis. The unexpected invasion of these systems by nanoparticles disturbs normal cell signaling, impairs cell and organ functions, and may even cause pathological disorders. This review examines the comprehensive health risks of exposure to nanoparticles by discussing how nanoparticles perturb various physiological systems as revealed by animal studies. The potential toxicity of nanoparticles to each physiological system and the implications of disrupting the balance among systems are emphasized.

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Expression changes of dopaminergic system-related genes in PC12 cells induced by manganese, silver, or copper nanoparticles

Cited by 170 publications

References 46 publications

Antibacterial activity of thin films TiO2 doped with Ag and Cu on Gracilicutes and Firmicutes bacteria

Antibacterial activity of thin films TiO2 doped with Ag and Cu on Gracilicutes and Firmicutes bacteria

Brain microvascular endothelial cell association and distribution of a 5 nm ceria engineered nanomaterial

Perturbation of physiological systems by nanoparticles

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