Expression characteristics of FHIT, p53, BRCA2 and MLH1 in families with a history of oesophageal cancer in a region with a high incidence of oesophageal cancer

Abstract

The aim of the present study was to determine the changes to the expression levels of fragile histidine triad (FHIT), breast cancer type 2 susceptibility protein (BRCA2), MutL homolog 1 (MLH1) and tumour protein 53 (p53) exhibited by families with a history of oesophageal cancer in a region that has a high incidence of oesophageal cancer, and to determine the association of these changes with the cancer history of the families. Immunohistochemistry was used to detect the protein expression of FHIT, p53, BRCA2, and MLH1 in the excised specimens of cancer tissues from 74 oesophageal cancer patients (positive family history of oesophageal cancer [OCFH +], n=33; negative family history of oesophageal cancer [OCFH −], n=41) from a region with a high incidence of oesophageal cancer. The positive expression rates of FHIT (61%; 45/74), BRCA2 (50%; 37/74) and MLH1 (27%; 9/33) in the oesophageal cancer tissues were significantly lower than those in the healthy tissues adjacent to the cancer (97% [29/30], 87% [26/30] and 73% [25/41], respectively). A significant difference was identified between the positive expression rates (P<0.01). However, FHIT, p53, BRCA2 and MLH1 expression demonstrated no significant affect on clinicopathological changes, such as oesophageal cancerous tissue differentiation, the degree of infiltration and cancer cell metastasis. The FHIT, BRCA2 and MLH1 expression levels were identified to be significantly lower in the cancer tissues from OCFH + patients. This result indicates that the expression levels of FHIT, BRCA2, and MLH1 are important molecular indices of genetic susceptibility to oesophageal cancer.

Expression of TP53, BCL-2, and VEGFA Genes in Esophagus Carcinoma and its Biological Significance

“…Vascular epithelial growth factor (VEGF) is also known to be related to the invasion and metastasis of malignant tumors. Studies have revealed the expression of TP53, BCL-2, and VEGFA genes in both esophagus tumor and adjacent tissues [ 5 , 6 ], suggesting the biological significance of those 3 factors in esophagus cancer. This study therefore investigated the effect of TP53, BCL-2, and VEGFA genes on the proliferation and apoptosis of esophagus carcinoma cells, via a methyl-benzyl-nitrosamine (MBNA)-induced rat esophagus cancer model, in an attempt to investigate the potential molecular mechanism underlying cancer pathogenesis.…”

FHIT Gene Expression in Acute Lymphoblastic Leukemia and its Clinical Significance

“…FHIT is believed to be a tumor suppressor gene, The FHIT gene at FRA3B is one of the earliest and most frequently altered genes in the majority of human cancers (Wang et al, 2014). The FHIT gene encodes a 1.1 kb mRNA which is expressed at low levels in most tissue types (ZHIWEI et al, 2015). Aberrant transcripts from this gene have been found in about half of all esophageal carcinomas, gastric carcinomas (Wang et al, 2014), and other carcinomas.…”

Identifying potential metastasis‐related long non‐coding RNAs, microRNAs, and message RNAs in the esophageal squamous cell carcinoma

“…In EC, high expression of hsa‐mir‐374a could promote proliferation via directly targeting apoptosis inducer Axin2 . Previous studies have revealed the expression of VEGFA in both esophagus tumor and adjacent tissues . It is found that VEGFA is downregulated and involved in the cell proliferation and epithelial‐to‐mesenchymal transition in esophageal adenocarcinoma .…”

“…52 Previous studies have revealed the expression of VEGFA in both esophagus tumor and adjacent tissues. 53,54 It is found that VEGFA is downregulated and involved in the cell proliferation and epithelial-tomesenchymal transition in esophageal adenocarcinoma. 55 In addition, VEGFA is involved in the pathways in cancer and Rap1 signaling pathway in ESCC.…”