Expression cloning of type 2 angiotensin II receptor reveals a unique class of seven-transmembrane receptors

Mukoyama, Masashi; Nakajima, Miki; Horiuchi, Masatsugu; Sasamura, Hiroyuki; Pratt, Richard E.; Dzau, Victor J.; Curnow, Kathleen M.

doi:10.1007/bf03349768

Cited by 223 publications

(322 citation statements)

References 20 publications

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“…Two kinds of Ang II receptors, AT1 and AT2, have been reported to exist in cardiac myocytes [15][16][17][18][19]. To examine which subtype of Ang II receptors is involved in the activation of p70 s6K, cardiac myocytes were preincubated with AT 1 receptor-specific antagonist, losartan (10 -5 M) or AT2 receptor-specific antagonist, PD123319 (10 -5 M) for 30 min and then stimulated with Ang II (10 -6 M) for 15 min.…”

Section: Ang H Stimulates P70 S6k Through At1 Receptormentioning

confidence: 99%

Activation of p70 S6 protein kinase is necessary for angiotensin II‐induced hypertrophy in neonatal rat cardiac myocytes

et al. 1996

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Although many lines of evidence have suggested that angiotensin II (Ang II) plays an important role in development of cardiac hypertrophy, the mechanism by which Ang II increases protein synthesis in cardiac myocytes remains unclear. It has been reported that the phosphorylation of $6 protein in 40 S ribosome is correlated to the efficiency of protein synthesis. In the present study, we have examined whether Ang II activates p70 $6 kinase (p70S6g), which has been reported to phosphorylate $6 protein.

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Section: Ang H Stimulates P70 S6k Through At1 Receptormentioning

confidence: 99%

Activation of p70 S6 protein kinase is necessary for angiotensin II‐induced hypertrophy in neonatal rat cardiac myocytes

et al. 1996

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“…For example, Ang 1-7 has vasodilatory and antihypertensive actions that oppose Ang II [6][7][8]. Ang II and Ang III have similar affinities for AT1 and AT2 receptors and cause vasoconstriction and promote aldosterone secretion [9][10][11]. Ang IV has been implicated in a number of physiological actions, including the regulation of blood flow, the modulation of exploratory behavior, and processes attributed to learning and memory [12].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous analysis of angiotensin peptides by LC–MS and LC–MS/MS: Metabolism by bovine adrenal endothelial cells

Cui

Nithipatikom

Campbell

2007

Analytical Biochemistry

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Liquid chromatography-electrospray ionization-mass spectrometry and liquid chromatographytandem mass spectrometry methods were developed to simultaneously determine the concentrations of angiotensin (Ang) II, Ang 1-7, Ang III and Ang IV in biological samples. The samples were extracted with C18 solid-phase extraction cartridges, and separated by a reverse-phase C18 column using acetonitrile in water with 0.1 % formic acid as a mobile phase. Angiotensin peptides were ionized by electrospray and detected by triple quadrupole mass spectrometry in the positive ion mode. (M+3H) 3+ and (M+2H) 2+ ions were chosen as the detected ions in the single ion recording (SIR) mode for LC-MS. The limits of detection (S/N=3) using SIR are 1 pg for Ang IV and 5 pg for Ang 1-7, Ang III and Ang II. Multiple reaction monitoring (MRM) mode was used for LC-MS/MS. The limits of detection (S/N=3) using MRM are 20 pg for Ang IV and 25 pg for Ang 1-7, Ang III and Ang II. These methods were applied to analyze angiotensin peptides in bovine adrenal microvascular endothelial cells. The results show that Ang II is metabolized by endothelial cells to Ang 1-7, Ang III and Ang IV with Ang 1-7 being the major metabolite.

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“…70 In contrast to the AT 1 receptor, much less is known about the structural and functional properties of the AT 2 receptor, which has been cloned recently from the mouse, rat, and human. 71,72 In all three species, the AT 2 -receptor cDNA encodes for a 363-amino acid receptor, and sequence comparison reveals an expectedly high homology on both the nucleic and amino acid level (human and rat AT 2 receptor cDNA share a homology of 89% and 91% on nucleic acid and amino acid level, respectively). Both mouse and human AT 2 receptor genes are located on the x chromosome.…”

Section: Development Of Ang Ii-receptor Binding Substancesmentioning

confidence: 99%

“…Conversely, Mukoyama et al 72 reported that the rat AT 2 receptor shares a seven transmembrane domain topology that may belong to a unique class of seven transmembrane receptors for which G-protein coupling has not been demonstrated. In their studies, stimulation of the cloned AT 2 receptor, transiently expressed in COS-7 cells, failed to increase IP 3 or intracellular calcium, and no apparent effects on cAMP and cGMP levels and phosphotyrosine phosphatase activity could be observed.…”

Section: Development Of Ang Ii-receptor Binding Substancesmentioning

confidence: 99%

Angiotensin II receptor pharmacology and AT1-receptor blockers

1999

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Expression cloning of type 2 angiotensin II receptor reveals a unique class of seven-transmembrane receptors

Cited by 223 publications

References 20 publications

Activation of p70 S6 protein kinase is necessary for angiotensin II‐induced hypertrophy in neonatal rat cardiac myocytes

Activation of p70 S6 protein kinase is necessary for angiotensin II‐induced hypertrophy in neonatal rat cardiac myocytes

Simultaneous analysis of angiotensin peptides by LC–MS and LC–MS/MS: Metabolism by bovine adrenal endothelial cells

Angiotensin II receptor pharmacology and AT1-receptor blockers

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