Expression Levels of EGF, TGF-α, and EGF-R Are Significantly Reduced in Pre-Implantation Cloned Mouse Embryos

Dadi, Tedla D.; Li, Ming W.; Lloyd, Kayna

doi:10.1089/1536230042323358

Cited by 2 publications

(2 citation statements)

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“…This is in keeping with previous evidence in the mouse that the earliest period of development is sensitive to the stress of individual culture (O'Neill, 1998). It is also in keeping with observations that the receptors of some growth factors and cytokines are expressed from the 2-cell stage or earlier (Dadi et al, 2004;Sharkey et al, 1995).…”

Section: Discussionsupporting

confidence: 91%

Combined effects of individual culture and atmospheric oxygen on preimplantation mouse embryos in vitro

Kelley

Gardner

2016

Reproductive BioMedicine Online

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Embryos are routinely cultured individually, although this can reduce blastocyst development. Culture in atmospheric (20%) oxygen is also common, despite multiple detrimental effects on embryos. Although frequently occurring together, the consequences of this combination are unknown. Mouse embryos were cultured individually or grouped, under physiological (5%) or atmospheric (20%) oxygen. Embryos were assessed by time-lapse and blastocyst cell allocation. Compared with the control group (5% oxygen group culture), 5-cell cleavage (t5) was delayed in 5% oxygen individual culture and 20% oxygen group culture (59.91 ± 0.23, 60.70 ± 0.29, 63.06 ± 0.32 h post-HCG respectively, P < 0.05). Embryos in 20% oxygen individual culture were delayed earlier (3-cell cleavage), and at t5 cleaved later than embryos in other treatments (66.01 ± 0.40 h, P < 0.001), this delay persisting to blastocyst hatching. Compared with controls, hatching rate and cells per blastocyst were reduced in 5% oxygen single culture and 20% oxygen group culture (134.1 ± 3.4, 104.5 ± 3.2, 73.4 ± 2.2 cells, P < 0.001), and were further reduced in 20% oxygen individual culture (57.0 ± 2.8 cells, P < 0.001), as was percentage inner cell mass. These data indicate combining individual culture and 20% oxygen is detrimental to embryo development.

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Section: Discussionsupporting

confidence: 91%

Combined effects of individual culture and atmospheric oxygen on preimplantation mouse embryos in vitro

Kelley

Gardner

2016

Reproductive BioMedicine Online

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“…Mitogenic growth factors have a profound influence on embryo development, growth, differentiation, and optimized embryo cleavage potential (Dadi et al, 2006). Dadi et al (2004) found that expression levels of epidermal growth factor (EGF), transforming growth factor α (TGF-α), and their cognate receptor, epidermal growth factor receptor (EGFR), were reduced significantly in preimplantation cloned mouse embryos. They demonstrated that adequate levels and appropriately timed expression of these factors and in turn EGFR activation were essential for ensuring appropriate cell proliferation in the preimplantation embryo development.…”

Section: Discussionmentioning

confidence: 99%

Number of blastomeres and distribution of microvilli in cloned mouse embryos during compaction

Wang

Zheng

et al. 2010

Zygote

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The events resulting in compaction have an important influence on the processes related to blastocyst formation. To analyse the quality of the embryos obtained by somatic cell nuclear transfer (SCNT) in aspects different from previous studies, not only the number of blastomeres of cloned embryos during the initiation of compaction, but also the distribution of microvilli in cloned, normal, parthenogenetic, and tetraploid embryos before and after compaction was preliminarily investigated in mouse. Our results showed that during compaction the number of blastomeres in SCNT embryos was fewer than that in intracytoplasmic sperm injection (ICSI) embryos and, before compaction, there was a uniform distribution of microvilli over the blastomere surface, but microvilli became restricted to an apical region after compaction in the four types of embryos. We also reported here that the time course of compaction in SCNT embryos was about 3 h delayed compared with that in ICSI embryos, while there was no significant difference between SCNT and ICSI embryos when developed to the 4-cell stage. We concluded that: (i) the cleavage of blastomeres in cloned embryos was slow at least before compaction; (ii) the distribution of microvilli in cloned, normal, parthenogenetic, and tetraploid embryos was coherent before and after compaction; and (iii) the initiation of compaction in SCNT embryos was delayed compared with that of ICSI embryos.

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Expression Levels of EGF, TGF-α, and EGF-R Are Significantly Reduced in Pre-Implantation Cloned Mouse Embryos

Cited by 2 publications

References 0 publications

Combined effects of individual culture and atmospheric oxygen on preimplantation mouse embryos in vitro

Combined effects of individual culture and atmospheric oxygen on preimplantation mouse embryos in vitro

Number of blastomeres and distribution of microvilli in cloned mouse embryos during compaction

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