Expression levels of FSHR, IGF1R, CYP11al and HSD3β in cumulus cells can predict in vitro developmental competence of bovine oocytes

Khurchabilig, Atchalalt; Sato, Akito; Ashibe, Shiori; Hara, Asuka; Fukumori, Rika; Nagao, Yoshikazu

doi:10.1017/s0967199420000283

Cited by 3 publications

(1 citation statement)

References 39 publications

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“…This is also called "oocyte fate" [142]. Although several studies in cattle already found several genes in CCs from germinal vesicle (GV) [16,35,[143][144][145][146][147][148][149][150][151] and MII oocytes [144,152] to be associated with oocyte competence, only a few reports matched the oocyte fate with the transcriptomic profile obtained from the CCs or granulosa cells (Table 2). There is some consensus regarding pathways correlated positively with oocyte competence, including the cell cycle (CCND1, CCNB2, and CCNA2 genes) [143,145,153], cell growth and proliferation, (CD44, TGFB1, EGF, FGF11, PRL, and GH genes) [35,[147][148][149]154], and steroidogenesis (HSD3B2 and CYP11A1 genes) [16,154].…”

Section: Transcriptomic and Proteomic Of Cumulus Cellsmentioning

confidence: 99%

Oocyte Selection for In Vitro Embryo Production in Bovine Species: Noninvasive Approaches for New Challenges of Oocyte Competence

Águila

Treulén

Therrien

et al. 2020

Animals

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The efficiency of producing embryos using in vitro technologies in livestock species rarely exceeds the 30–40% threshold, indicating that the proportion of oocytes that fail to develop after in vitro fertilization and culture is considerably large. Considering that the intrinsic quality of the oocyte is one of the main factors affecting blastocyst yield, the precise identification of noninvasive cellular or molecular markers that predict oocyte competence is of major interest to research and practical applications. The aim of this review was to explore the current literature on different noninvasive markers associated with oocyte quality in the bovine model. Apart from some controversial findings, the presence of cycle-related structures in ovaries, a follicle size between 6 and 10 mm, large number of surrounding cumulus cells, slightly expanded investment without dark areas, large oocyte diameter (>120 microns), dark cytoplasm, and the presence of a round and smooth first polar body have been associated with better competence. In addition, the combination of oocyte and zygote selection via brilliant cresyl blue (BCB) test, spindle imaging, and the anti-Stokes Raman scattering microscopy together with studies decoding molecular cues in oocyte maturation have the potential to further optimize the identification of oocytes with better developmental competence for in-vitro-derived technologies in livestock species.

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Section: Transcriptomic and Proteomic Of Cumulus Cellsmentioning

confidence: 99%

Oocyte Selection for In Vitro Embryo Production in Bovine Species: Noninvasive Approaches for New Challenges of Oocyte Competence

Águila

Treulén

Therrien

et al. 2020

Animals

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Gene expression levels in cumulus cells are correlated with developmental competence of bovine oocytes

Anazawa,

Ashibe,

Nagao

2025

Theriogenology

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Morpho-molecular evaluation for developmental competence of oocytes retrieved through transvaginal ovum pick-up from FSH-stimulated Tharparkar donor cows (Bos indicus)

Donadkar,

Kumar,

Singh

et al. 2024

Zygote

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Summary The study was conducted on indigenous Tharparkar cow (Bos indicus) to evaluate FSH stimulation on follicular attributes, oocyte recovery and morpho-molecular developmental competence parameters concerning oocyte quality. A total of 20 OPU sessions were performed, which included 10 sessions in each FSH stimulated at the dose of 130 µg divided into four sub-doses and non-stimulated. Findings on the size of follicles having ≥6 mm showed a significantly higher, however an opposite trend was observed in the case of smaller sized follicle (<6 mm) between stimulated and non-stimulated respectively. The stimulated cows had a significantly higher number as well as the percentage of oocytes of Grade A, having a diameter ≥120 µm and BCB+VE as compared to the non-stimulated cows. The relative mRNA expression profile of GDF9, BMP15, PCNA and BCL-2 genes was higher and BAX was lower in the FSH-stimulated cow. These results indicated that FSH stimulation before OPU in Bos indicus cows has a significant impact on follicle size, oocyte yield, recovery, and their quality with respect to COC’s, diameter and BCB+VE oocytes. Further, a significant increase in the relative mRNA expression levels of GDF9, BMP15 and PCNA genes in the FSH-stimulated group suggests that FSH plays a key role in modulating the expression of these important candidate genes and thus influencing oocyte quality. The higher mRNA expression of BCL-2 genes and concomitantly lower expression of BAX gene in FSH Stimulated cows indicates the protective role of these genes and preventing programmed cell death and thus promoting cell survival, quality and embryo development.

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Expression levels of FSHR, IGF1R, CYP11al and HSD3β in cumulus cells can predict in vitro developmental competence of bovine oocytes

Cited by 3 publications

References 39 publications

Oocyte Selection for In Vitro Embryo Production in Bovine Species: Noninvasive Approaches for New Challenges of Oocyte Competence

Oocyte Selection for In Vitro Embryo Production in Bovine Species: Noninvasive Approaches for New Challenges of Oocyte Competence

Gene expression levels in cumulus cells are correlated with developmental competence of bovine oocytes

Morpho-molecular evaluation for developmental competence of oocytes retrieved through transvaginal ovum pick-up from FSH-stimulated Tharparkar donor cows (Bos indicus)

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