Expression of 15-lipoxygenase type-1 in human mast cells

Gulliksson, Magdalena; Brunnström, Åsa; Johannesson, Malin; Backman, Linda; Nilsson, Gunnar; Harvima, Ilkka T.; Dahlén, Barbro; Kumlin, Maria; Claesson, Hans‐Erik

doi:10.1016/j.bbalip.2007.06.001

Cited by 53 publications

(80 citation statements)

References 33 publications

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“…1). The retention times of the material in these five peaks corresponded to the retention times for the two double oxygenation products 8(S),15(S)-5,9,11,13(Z,E,Z,E)-DiHETE and 8(R),15(S)-5,9,11,13(Z,E,Z,E)-DiHETE, the two products 8(S),15(S)-5,9,11,13(Z,E,E,E)-DiHETE and 8(R),15(S)-5,9,11,13(Z,E,E,E)-DiHETE derived from 14,15-oxido-LTA 4 (19,20) and 14(R),15(S)-DiHETE, respectively. The pattern of products formed was similar to that reported for human airway epithelial cells incubated with arachidonic acid (5).…”

Section: Formation Of 15lo Products In Isolated Humanmentioning

confidence: 99%

“…The amino acid sequence similarity between these enzymes is only 40% (3). IL-4 and/or IL-13 induce the expression of 15-LO-1 in cultured mast cells, monocytes, and epithelial cells (4,8,9). Thus, these cytokines may act as important physiological/pathophysiological regulators of 15-LO-1 expression in vivo.…”

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confidence: 99%

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confidence: 99%

“…5-Lipoxygenase (LO) is the key enzyme in leukotriene (LT) synthesis, and this enzyme catalyzes the conversion of arachidonic acid to LTA 4 . This unstable metabolite can be hydrolyzed enzymatically to LTB 4 or conjugated with glutathione to form LTC 4 , which in turn can be metabolized to LTD 4 and LTE 4 . LTC 4 and its metabolites LTD 4 and LTE 4 are collectively referred to as cysteinyl LT, and these mediators are proinflammatory agents and potent bronchoconstrictors (1).…”

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confidence: 99%

“…In contrast to 5-LO, little is known about the biological function of human 15-LO. Human airway epithelial cells, eosinophils and subsets of mast cells and dendritic cells contain high amounts of 15-LO-1 (2)(3)(4)(5)(6). A second form of human 15-LO also exists, which is named 15-LO-2 (7).…”

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confidence: 99%

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Eoxins are proinflammatory arachidonic acid metabolites produced via the 15-lipoxygenase-1 pathway in human eosinophils and mast cells

Feltenmark¹,

Gautam²,

Brunnström³

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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Human eosinophils contain abundant amounts of 15-lipoxygenase (LO)-1. The biological role of 15-LO-1 in humans, however, is unclear. Incubation of eosinophils with arachidonic acid led to formation of a product with a UV absorbance maximum at 282 nm and shorter retention time than leukotriene (LT)C4 in reverse-phase HPLC. Analysis with positive-ion electrospray tandem MS identified this eosinophil metabolite as 14,15-LTC4. This metabolite could be metabolized to 14,15-LTD4 and 14,15-LTE4 in eosinophils. Because eosinophils are such an abundant source of these metabolites and to avoid confusion with 5-LO-derived LTs, we suggest the names eoxin (EX)C4, -D4, and -E4 instead of 14,15-LTC4, -D4, and -E4, respectively. Cord blood-derived mast cells and surgically removed nasal polyps from allergic subjects also produced EXC4. Incubation of eosinophils with arachidonic acid favored the production of EXC4, whereas challenge with calcium ionophore led to exclusive formation of LTC4. Eosinophils produced EXC4 after challenge with the proinflammatory agents LTC4, prostaglandin D2, and IL-5, demonstrating that EXC4 can be synthesized from the endogenous pool of arachidonic acid. EXs induced increased permeability of endothelial cell monolayer in vitro, indicating that EXs can modulate and enhance vascular permeability, a hallmark of inflammation. In this model system, EXs were 100 times more potent than histamine and almost as potent as LTC4 and LTD4. Taken together, this article describes the formation of proinflammatory EXs, in particular in human eosinophils but also in human mast cells and nasal polyps.

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Section: Formation Of 15lo Products In Isolated Humanmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Eoxins are proinflammatory arachidonic acid metabolites produced via the 15-lipoxygenase-1 pathway in human eosinophils and mast cells

Feltenmark¹,

Gautam²,

Brunnström³

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

149

145

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Electrochemical oxidation of phosphatidylethanolamines studied by mass spectrometry

Colombo

Coliva

Kraj³

et al. 2018

J Mass Spectrom

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Phosphatidylethanolamines (PEs) are widely present in cellular membranes and lipoproteins. Oxidation of PE fatty acyl chains generates several oxidized products, exerting a vast number of biological functions, not totally unveiled yet. In vitro biomimetic models have been used to identify oxidized PEs and to develop analytical strategies for their targeted detection in vivo. Most of the models are based on oxidation by reactive oxygen species (ROS), but the oxidative metabolism of PE also relies on controlled reactions catalyzed by enzymes as lipoxygenase, which can be mimicked by electrochemical (EC) oxidation. In this study, 3 PE standards (1‐palmitoyl‐2‐oleoyl‐sn‐glycero‐3‐phosphoethanolamine (POPE), 1‐palmitoyl‐2‐linoleoyl‐sn‐glycero‐3‐phosphoethanolamine (PLPE), and 1‐palmitoyl‐2‐arachidonoyl‐sn‐glycero‐3‐phosphoethanolamine (PAPE)) were oxidized by EC oxidation, using an EC flow‐through cell system as a biomimetic model of oxidative injury. The new oxidation products were identified by online EC electrospray ionization mass spectrometry (EC‐ESI‐MS and MS/MS). Long‐chain and short‐chain oxidation products were identified, bearing modifications in the sn‐2 acyl chains, whereas the oxidation pattern was dependent on the unsaturation level. Long‐chain oxidation products of PEs (keto, hydroxy, hydroperoxy, poly‐hydroperoxy derivatives) were identified, bearing up to 5, 7, and 10 oxygens for POPE, PLPE, and PAPE, respectively. Fourteen short‐chain oxidation products, 7 from PLPE, and 7 from PAPE, including aldehydes, γ‐hydroxy‐α,β‐aldehydes, and dicarboxylic acids were characterized. Some of these oxidized species were previously reported during the oxidative metabolism of PEs driven by ROS. The EC‐ESI‐MS platform was, therefore, able to mimic the oxidative metabolism of PEs mediated by ROS.

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Influence of Polyphenols on the Physiological Processes in the Skin

Ratz-Łyko

Arct

Majewski

et al. 2015

Phytotherapy Research

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In the last decade antioxidants from a group of polyphenols have been proposed as one of the most effective functional ingredients of anti-ageing properties that counteract the effects of oxidative damage to the skin. It has been shown that the use of polyphenols affects skin protection and mitigates inflammatory conditions of the skin. Numerous studies have confirmed that polyphenols by neutralizing free radicals, antioxidant activity and by their ability to chelate ions of transition metals can effectively reduce the level of nonprotein inflammatory mediators. The biological activity of polyphenols in the skin is primarily determined by their physicochemical properties and the ability to overcome the epidermal barrier as they try to reach appropriate receptors. This study reviews literature on the effects of polyphenols relating to the physiological processes in the skin and role of the major plant polyphenols in cosmetology and dermatology.

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Expression of 15-lipoxygenase type-1 in human mast cells

Cited by 53 publications

References 33 publications

Eoxins are proinflammatory arachidonic acid metabolites produced via the 15-lipoxygenase-1 pathway in human eosinophils and mast cells

Eoxins are proinflammatory arachidonic acid metabolites produced via the 15-lipoxygenase-1 pathway in human eosinophils and mast cells

Electrochemical oxidation of phosphatidylethanolamines studied by mass spectrometry

Influence of Polyphenols on the Physiological Processes in the Skin

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