Expression of a bacterial gene in plants by using a viral vector

Brisson, Normand; Paszkowski, Jerzy; Penswick, John Robert; Gronenborn, Bruno; Potrykus, I.; Höhn, Thomas

doi:10.1038/310511a0

Cited by 203 publications

(70 citation statements)

References 20 publications

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“…Among the notable achievements were the ¢rst transfection of plant protoplasts with infectious RNA transcripts of brome mosaic virus (Ahlquist et al 1984), the synthesis of recombinant marker proteins in cells and inoculated leaves (Brisson et al 1984;French et al 1986;Takamatsu et al 1987;Dawson et al 1988) and the production of measurable quantities of a cytokine with biological activity (de Zoeten et al 1989). However, the use of recombinant plant viruses as research and production tools for plant biotechnology was not ¢rmly established until tobamovirus vectors were used in a commercial context to transfect whole plants and the resulting products puri¢ed on a pilot scale and analysed for their composition and speci¢c activity.…”

Section: Introductionmentioning

confidence: 99%

Tobacco mosaic virus and the virescence of biotechnology

Turpen¹

1999

Phil. Trans. R. Soc. Lond. B

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There is a growing realization that a modern combination of molecular biology and agriculture will provide a photosynthetic basis for the biosynthesis of an increasing variety of complex and valuable molecules. This`greening' of biotechnology may impact on the global environment in many bene¢cial ways, but will perhaps have its most signi¢cant impact on human health. In the past decade, the capacity to use plants as an expanded source of therapeutics has grown through the accelerated development of e¡ective viral transfection vectors for gene transfer to cultivated crops. Recombinant vectors based on tobacco mosaic virus (TMV) and other members of the Tobamovirus genus are now used to transfect commercially meaningful quantities of plant biomass cultivated in enclosed greenhouses and multiacre ¢elds. Viral RNA promoters are e¡ectively manipulated for the synthesis of recombinant messenger RNAs in whole plants. Chimeric plant virus and virus-like particles are designed for peptide production and display from recombinant structural protein^gene fusions. Gene functions are assessed and modi¢ed by either virus-mediated expression or cytosolic inhibition of expression at the RNA level. Recombinant virus populations, propagated by inoculating plants with infectious RNA transcripts or recombinant virions, have proved to be genetically stable over product-manufacturing cycles. Large volumes of highly puri¢ed protein products isolated from transfected foliage conform reproducibly to the speci¢cations required for well-characterized biologics. In some cases, they exceed the speci¢c activities of molecules puri¢ed from alternative recombinant and native sources. The resulting products are then formulated according to the developing national regulatory guidelines appropriate for agriculture-based manufacturing. Each of these innovations was ¢rst realized by researchers using clones of tobamovirus genes and recombinant genomes. This progress is founded on the heritage of a century of fundamental TMV research.

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Section: Introductionmentioning

confidence: 99%

Tobacco mosaic virus and the virescence of biotechnology

Turpen¹

1999

Phil. Trans. R. Soc. Lond. B

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“…CaMV is known to tolerate small insertions in the gene II region, in which they do not affect the viability of the virus (Gronenborn et al, 1981;Brisson et al, 1984;Bakkeren et al, 1989). We took advantage of this and introduced short oligonucleotides into this region.…”

Section: Discussionmentioning

confidence: 99%

“…To facilitate discussion we arbitrarily define mutant types carrying existing and new mutations (produced by in vitro manipulation of the DNA sequence in the dispensable gene II region) using the letters A, B, C and D. All are derivatives of strain CM4-184 Gardner et al, 1981). Types A and B have already been described [Bakkeren et al, 1989, in which they are named Ca355 and Ca355THBI, respectively; Ca355 (Brisson et al, 1984) has a 12 bp intergenic region between open reading frames I and III that contains a XhoI site]. Type B carries an oligonucleotide (not present in type A) that can be cut with EcoRV (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Its life cycle and potential use as a vector in genetic engineering of plants have been investigated (Gronenborn et al, 1981 ;Hohn et al, 1982Hohn et al, , 1987Brisson et al, 1984;Gronenborn, 1987). Following infection, the nick-overlap triple helices remaining in the viral DNA as vestiges of the previous replication cycle are first repaired in the host cell nucleus to produce a closed double-stranded circular genome (Howell & Hull, 1978;Ansa et al, 1982;Hull & Covey, 1983a;Maule, 1985).…”

Section: Introductionmentioning

confidence: 99%

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The mode of cauliflower mosaic virus propagation in the plant allows rapid amplification of viable mutant strains

Riederer

Grimsley²,

Höhn³

et al. 1992

Journal of General Virology

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We inoculated the leaves of turnip plants (Brassica campestris spp. rapa cv. Just Right) with two cauliflower mosaic viruses (CaMVs) with different small mutations in a dispensable region of the viral genome, and followed the spread of the virus infection through the plant. Surprisingly, analysis of viral DNA in single primary chlorotic lesions revealed the presence of both mutants. In contrast, the secondary chlorotic lesions and systemically infected leaves contained virus molecules of either one or the other type only. Infection of plants with different ratios of the two reporter viruses showed that this ratio is not conserved during systemic virus spread. Infection with CaMV DNA in the form of heteroduplexes containing a single mismatched base pair, in which each strand carried a distinct diagnostic marker, provided us with evidence that the mismatch was subjected to a repair process in the host plant.

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“…Biolistic and Agrobacterium-mediated transformation methods have been widely used to generate many transgenic plants with genes of industrial or agronomic interest. During the early phase of plant genetic engineering, certain constitutive promoters, such as the cauliflower mosaic virus 35S promoter and maize ubiquitin, have been used to express a wide range of traits in various plant species (Brisson et al, 1984;Cornejo et al, 1993). However, constitutive or overexpression strategies may lead to undesirable pleiotropic effects in transgenic plants (Hsieh et al, 2002;Kasuga et al, 1999).…”

Section: Introductionmentioning

confidence: 99%