Expression of a deregulated tobacco nitrate reductase gene in potato increases biomass production and decreases nitrate concentration in all organs

Djennane, Samia; Quilleré, Isabelle; Leydecker, Marie‐Thérèse; Meyer, Christian; Chauvin, Jean-Éric

doi:10.1007/s00425-004-1287-5

Cited by 18 publications

(9 citation statements)

References 23 publications

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“…Moreover, we noted two interesting features of this cultivar: -relatively high activity of nitrate reductase at two final harvests (142 and 178 DAS) in comparison to earlier phases, and -high concentration of total chlorophyll during the whole growing season (particularly in the plants emerged from both energ'hill seeds and seeds treated by laser D5). Djennane et al (2004) stated that the enhancement of the nitrate reduction rate may lead to higher biomass production. Chen et al (2005b) showed that laser pretreatment induces statistically significant changes in some biochemical and physiological parameters in seedlings of Isatis indogotica.…”

Section: Resultsmentioning

confidence: 99%

Impact of presowing laser irradiation of seeds on sugar beet properties

Sacała¹,

Demczuk²,

Grzys³

et al. 2012

International Agrophysics

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A b s t r a c t. The aim of the experiment was to establish the influence of biostimulation on the sugar beet seeds. The seeds came from the specialized breeding program energ'hill or were irradiated by the laser in two doses. The impact of the biostimulation was analyzed by determining the nitrate reductase activity and the nitrate, chlorophyll and carotenoids contents in leaves, as well as, the dry matter and sugar concentration in mature roots. The field experiment was established for two sugar beet cultivars. Biostimulation by irradiation and a special seed breeding program energ'hill had a positive influence on some examined parameters (particularly on nitrate reductase activity in Ruveta and in numerous cases on photosynthetic pigments in both cultivars). Regarding the dry matter accumulation and sugar concentration this impact was more favourable for Tiziana than for Ruveta cultivar.K e y w o r d s:

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Section: Resultsmentioning

confidence: 99%

Impact of presowing laser irradiation of seeds on sugar beet properties

Sacała¹,

Demczuk²,

Grzys³

et al. 2012

International Agrophysics

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“…The higher nitrate content in the HNR genotype might be due to its better nitrate uptake capacity. It is also noteworthy that the HNR genotype produced more than twice as much biomass as the LNR genotype possibly because of its higher nitrate Downloaded by [University of Saskatchewan Library] at 11:12 19 November 2014 reduction rate, thus allowing a better allocation of nitrogen resources to photosynthesis and carbon metabolism (Djennane et al, 2004). These findings are in consonance with the view that the plant's capacity for biomass production and nitrogen uptake may be highly dependent on environmental conditions including the nitrate supply (Lawlor, 2002).…”

Section: Discussionmentioning

confidence: 98%

Modulation of Nitrogen-Utilization Efficiency in Wheat Genotypes Differing in Nitrate Reductase Activity

[]

Umar

Abrol

et al. 2011

Journal of Plant Nutrition

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2 The plants growing in natural field conditions do not express their full genetic potential of nitrogen (N) utilization due to a limiting availability of N at later stages of growth. Their full potential is likely to manifest under non-limiting nitrogen supply wherein the high nitrate reductase (HNR) and the low nitrate reductase (LNR) genotypes should differ significantly in their N-utilization efficiencies. In a sand culture experiment, using IC 321157 (HNR) and C 306 (LNR) genotypes of Triticum aestivum L. under controlled conditions, 15-day-old plants were collected in triplicate and analyzed for nitrate content, N-metabolizing enzymes and N harvest. Kinetic studies were conducted to obtain the Km and Vmax values for enzymes. The values for nitrate content, activities of the nitrate-and the ammonium-assimilating enzymes, biomass and N harvest were higher in the HNR than in the LNR genotype. The higher affinities of enzymes to their substrates in the HNR genotype indicated a greater potential of this genotype for N utilization under non-limiting N supply with a well-coordinated system of N uptake and assimilation. The study suggests that the N-utilization efficiency of plants can be improved by exploiting their full genetic potential under non-limiting N supply, which may be achieved by synchronizing the supply with demand during late stages of plant growth. It also shows that the enzymes responsible for N assimilation act in a coordinated way, thus necessitating the need of a holistic approach for the study of the N-metabolic pathways.

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“…In another variety of potato, the transgenic plants showed a marked improvement in biomass production, especially in tubers, with still lower amounts of nitrate. The more effective reduction of nitrate probably allowed a better allocation of N to the photosynthetic apparatus and to enzymes involved in C metabolism, which was demonstrated by higher leaf chlorophyll content in the transgenic potato plants [173].…”

Section: Improvement Of Nitrogen Utilization Using Genetically Modifimentioning

confidence: 99%

Improving Nitrogen Use Efficiency in Crops for Sustainable Agriculture

et al. 2011

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Abstract:In this review, we present the recent developments and future prospects of improving nitrogen use efficiency (NUE) in crops using various complementary approaches. These include conventional breeding and molecular genetics, in addition to alternative farming techniques based on no-till continuous cover cropping cultures and/or organic nitrogen (N) nutrition. Whatever the mode of N fertilization, an increased knowledge of the mechanisms controlling plant N economy is essential for improving NUE and for reducing excessive input of fertilizers, while maintaining an acceptable yield and sufficient profit margin for the farmers. Using plants grown under agronomic conditions, with different tillage conditions, in pure or associated cultures, at low and high N mineral fertilizer input, or using organic fertilization, it is now possible to develop further whole plant agronomic and physiological studies. These can be combined with gene, protein and metabolite profiling to build up a comprehensive picture depicting the different steps of N uptake, assimilation and recycling to produce either biomass in vegetative organs or proteins in storage organs. We provide a critical overview as to how our understanding of the agro-ecophysiological, physiological and molecular controls of N assimilation in crops, under varying environmental conditions, has been improved. We have used combined approaches, based on agronomic studies, whole plant physiology, quantitative genetics, forward and reverse genetics and the emerging systems biology. Long-term sustainability may require a gradual transition from synthetic N inputs to legume-based crop rotation, including continuous cover cropping systems, where these may be possible in certain areas of the world, depending on climatic conditions. Current knowledge and prospects for future agronomic development and application for breeding crops adapted to lower mineral fertilizer input and to alternative farming techniques are explored, whilst taking into account the constraints of both the current world economic situation and the environment.

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Expression of a deregulated tobacco nitrate reductase gene in potato increases biomass production and decreases nitrate concentration in all organs

Cited by 18 publications

References 23 publications

Impact of presowing laser irradiation of seeds on sugar beet properties

Impact of presowing laser irradiation of seeds on sugar beet properties

Modulation of Nitrogen-Utilization Efficiency in Wheat Genotypes Differing in Nitrate Reductase Activity

Improving Nitrogen Use Efficiency in Crops for Sustainable Agriculture

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