Expression of a fungal cyanamide hydratase in transgenic soybean detoxifies cyanamide in tissue culture and in planta to provide cyanamide resistance

Genetically Modified Soybean for Insect-Pests and Disease Control

“…The active toxins can interact with specific receptors at the midgut, leading to pore formation after toxin conformation change and, consequently, cell death (GILL et al, 1992;KNOWLES, 1994;RAJAMOHAN et al, 1998). Although this is the most commonly suggested method of action for Cry toxins, details about this mechanism, such as pos-binding effects and receptor specificities, are not yet clear (GOMEZ et al, 2002;ZHANG et al, 2005).…”

Advances in selectable marker genes for plant transformation

“…The enzyme has extremely narrow substrate specificity. The use of cyanamide hydratase as a selectable marker has been demonstrated in wheat (Weeks et al, 2000), tobacco (Maier-Greiner et al, 1991b;Kirubakaran and Sakthivel, 2008), potato, tomato, rice, Arabidopsis (Damm, 1998) and soybean (Zhang et al, 2005;Ulanov and Widholm, 2007). In a recent study, the heterologous expression of cah marker gene was reported.…”

Specificity of expression of the GUS reporter gene (uidA) driven by the tobacco ASA2 promoter in soybean plants and tissue cultures

“…Jack] were initiated and maintained as described by Zhang et al (2005). The cultures were bombarded, selected with hygromycin, plants were regenerated and leaf segments were tested for hygromycin resistance at each generation as described by Zhang et al (2005).…”

“…The untransformed control Jack did not show any hybridizing fragment. Clearly the bombardment protocol used here generally inserts multiple gene copies as we described before (Zhang et al, 2005). The fragments are of variable sizes since there is one unique HindIII site in the vectors (Fig.…”