Expression of a Neural Stem/Progenitor Cell Marker Nestin in Salivary Glands

Yokoyama, Megumi; Katsumata-Kato, Osamu; Sakurai, Hiroyuki; Ogawa, Hiroaki; Fujita-Yoshigaki, Junko

doi:10.15406/mojap.2017.04.00133

Cited by 3 publications

(7 citation statements)

References 21 publications

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“…Nestin is an intermediate filament protein expressed in the central nervous system stem and progenitor cells 25) , but its expression has also been reported in non-neural stem cells [26][27][28] . The pancreas, liver, and salivary glands are endoderm-derived similar glandular tissues, and these tissues have been reported to have similar structures, functions, and molecular markers 29,30) . Therefore, the regenerative process in the pancreas and liver is very similar to that of salivary glands.…”

Section: Discussionmentioning

confidence: 99%

“…Nestin is also a marker of pluripotent stem cells in the central nervous system. Yokoyama et al 29) reported an increase in nestin expression in atrophic acinar cells during the recovery process following unilateral parotid ligation and suggested that it might be a useful marker of salivary gland progenitor cells. However, in this study, there was no significant increase in nestin mRNA levels, and immunostaining revealed nestin-positive reactions in endothelial cells of blood vessels and nerve fibers but not in cells constituting acini or ducts.…”

Section: Discussionmentioning

confidence: 99%

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Examination of Changes in the Remaining Submandibular Gland after Resection of the Contralateral Salivary Gland

Miyasaka

Ikeda

Hirashima

et al. 2023

J. Hard Tissue Biology.

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Salivary glands are exocrine glands that secrete saliva, which plays an important role in the maintenance of oral health. Few reports exist on the contralateral salivary gland that is not directly affected when one salivary gland is affected by sialolithiasis, radiation therapy, or a tumor. In the present study, we investigated the changes in the contralateral submandibular gland in mice following the excision of unilateral salivary gland. Outcomes of this study showed that the unilateral submandibular and sublingual glands resection resulted in a significant increase in the weight of the remaining submandibular gland after 21 days following the unilateral resection of salivary gland. The appearance rate of the proliferating cells by cell type constituting the submandibular gland parenchyma significantly increased in the acinar cells of the contralateral submandibular gland, 7 days after unilateral resection of the submandibular and sublingual glands. This suggests that the unilateral submandibular and sublingual glands resection resulted in proliferation of the acinar cells in the contralateral submandibular gland. The area of the acini comprising the submandibular gland significantly increased in the remaining submandibular gland after 21 days following unilateral salivary gland resection, suggesting that the acinar cells proliferated as early as 7 days after salivary gland resection, resulting in increased acinar area and submandibular gland weight at 21 days post resection. The increased weight of the remaining contralateral submandibular gland may be attributable to the resection, owing to the proliferation and increased area of the acinar cells to compensate for the loss of salivary gland function.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Examination of Changes in the Remaining Submandibular Gland after Resection of the Contralateral Salivary Gland

Miyasaka

Ikeda

Hirashima

et al. 2023

J. Hard Tissue Biology.

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“…Salivary glands recover when the ligation is released 7 days after the duct ligation [19,20]. We previously reported that parotid gland morphology showed weak atrophy of acinar cells in some samples on day 3 of duct ligation, whereas most acinar cells in the parotid gland were atrophied on day 7 of duct ligation [18]. Therefore, the period of duct ligation was set at 7 days for the present study.…”

Section: Ductmentioning

confidence: 97%

“…Mice were injected intraperitoneally with a mixture of medetomidine hydrochloride (0.3 mg/kg), midazolam (4 mg/kg), and butorphanol tartrate (5 mg/kg). Under anesthesia, the unilateral parotid excretory duct was ligated, according to a previously reported procedure [18]. Salivary glands recover when the ligation is released 7 days after the duct ligation [19,20].…”

Section: Ductmentioning

confidence: 99%

Acinar Cell Proliferation Promoted by BMP2 in Injured Mouse Parotid Gland: BMP2 Promotes Cell Proliferation in Parotid Gland

Yokoyama

Katsumata-Kato

Fujita-Yoshigaki

2023

International Journal of Dentistry

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Objective. To identify factors that affect salivary gland recovery, we investigated the expression and function of bone morphogenetic protein 2 (BMP2) in mice. Materials and Methods. Using a micro clip, mice parotid glands were removed 7 days after the ligation of the unilateral parotid excretory duct. Thereafter, they were weighed and stained with hematoxylin and eosin, and BMP2 expression was examined via real-time reverse transcription-polymerase chain reaction. Primary cultures of parotid glands were prepared, and BMP2 protein was added to the culture medium for 48 hr to examine its effect on cell proliferation. E-cadherin and vimentin expression was examined using western blotting. Finally, immunohistochemical staining using an anti-Ki67 antibody was performed. Results. Duct-ligated parotid glands weighed less than those that were collected after sham surgery and showed acinar cell atrophy. They also showed higher BMP2 expression than control glands. Primary-cultured parotid acinar cells supplemented with BMP2 showed higher proliferative potential than control cells. Furthermore, they showed E-cadherin, but not vimentin, expression, and their percentage of Ki67-positive cells were higher than that corresponding to the controls. Conclusions. Injury to salivary glands by excretory duct ligation increased BMP2 expression, which may be involved in maintaining salivary gland function by inducing acinar cell proliferation.

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“…KEGG pathways that were identified from combination analysis of the 7 down-regulated miRNAs and mRNA expression microarray expression of nestin and cytokeratin-14, stem/progenitor markers, increased during the culture of parotid acinar cells (4,6). In addition, ligation of parotid excretory duct induced expression of nestin and cytokeratin-5 (21,22). After injury, various epithelial cells begin to express nestin, which may indicate a reversion to an immature phenotype.…”

Section: Change In the Expression Level Of Rno-mir-3473 In Parotid Acmentioning

confidence: 99%

Change in Expression Patterns of Micro RNAs in the Primary Culture of Parotid Acinar Cells

et al. 2019

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Micro RNAs(miRNAs)are considered to regulate the development and dysfunction of salivary glands. Previous studies on the expression patterns of genes in the primary culture of parotid acinar cells revealed that the isolation process of acinar cells from the glands caused tissue injuries and activated the Src-p38 MAP kinase signaling pathway. While acinar markers were decreased, expression of stem/progenitor markers commenced in the culture. In this study, changes in expression patterns of miRNAs of parotid acinar cells were examined by microarray analysis in order to clarify the mechanism for changes in characteristics of the cells. Analysis on expression values after normalized with robust multi-array averaging method identified that 18 miRNAs were up-regulated and 52 miRNAs were down-regulated after 1-day culture compared with just after isolation. Pathway analysis on the expression-altered miRNAs suggested that they were involved in the regulation of multipotent stem cells. Among the down-regulated miRNAs that were highly expressed, miR-3473, whose fold change was the largest, significantly decreased during the culture for 2 days. Inversely, Sox10, a candidate of target genes for miR-3473, increased during the culture, which was confirmed by real time RT-PCR and immunoblot analyses. Inhibition of Src kinase activity suppressed the changes in expression levels of miR-3473 and Sox10. Since Sox10 is involved in maintenance of stemness and promotes nestin expression, there is a possibility that the down-regulation of miRNAs may regulate acquisition of stemness in parotid acinar cells.

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Expression of a Neural Stem/Progenitor Cell Marker Nestin in Salivary Glands

Cited by 3 publications

References 21 publications

Examination of Changes in the Remaining Submandibular Gland after Resection of the Contralateral Salivary Gland

Examination of Changes in the Remaining Submandibular Gland after Resection of the Contralateral Salivary Gland

Acinar Cell Proliferation Promoted by BMP2 in Injured Mouse Parotid Gland: BMP2 Promotes Cell Proliferation in Parotid Gland

Change in Expression Patterns of Micro RNAs in the Primary Culture of Parotid Acinar Cells

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