2005
DOI: 10.6090/jarq.39.11
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Expression of a Non-Secreted Form of Juvenile Hormone Esterase in a Baculovirus

Abstract: Pericardial cells rapidly cleared recombinant-juvenile hormone esterase (rJHE) expressed within a baculovirus in insects from the hemolymph. To prevent the clearance of rJHE, we used the polymerase chain reaction (PCR) to remove the signal sequence from the JHE gene, thereby converting the enzyme to a non-secreted form (NSJHE). The resulting gene was expressed in a baculovirus (AcN-SJHE) using HIGH-FIVE cells and proper cellular enzyme production was monitored. Transcription level of the NSJHE and rJHE were co… Show more

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