Expression of a novel cardiac-specific tropomyosin isoform in humans

Denz, Christopher R.; Narshi, Aruna; Zajdel, Robert W.; Dube, Dipak K.

doi:10.1016/j.bbrc.2004.06.084

Cited by 64 publications

(94 citation statements)

References 13 publications

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“…Because ␣/2a is high in blood vessels, the transcripts detected by Cooley and Bergtrom (2001) may actually come from vascular tissue in these organs. Recently, a novel cardiac-specific Tm containing the 2a exon from the ␣-gene was detected in human heart using PCR amplification (Denz et al 2004) and has also been detected in axolotl heart (Zajdel et al 2002). This isoform contains the 9a/9b C terminus like striated Tm but with 2a in place of 2b.…”

Section: Discussionmentioning

confidence: 99%

Smooth Muscle-specific α Tropomyosin Is a Marker of Fully Differentiated Smooth Muscle in Lung

Vrhovski

McKay

Schevzov

et al. 2005

J Histochem Cytochem.

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S U M M A R Y Tropomyosin (Tm) is one of the major components of smooth muscle. Currently it is impossible to easily distinguish the two major smooth muscle (sm) forms of Tm at a protein level by immunohistochemistry due to lack of specific antibodies. ␣ -sm Tm contains a unique 2a exon not found in any other Tm. We have produced a polyclonal antibody to this exon that specifically detects ␣ -sm Tm. We demonstrate here the utility of this antibody for the study of smooth muscle. The tissue distribution of ␣ -sm Tm was shown to be highly specific to smooth muscle. ␣ -sm Tm showed an identical profile and tissue colocalization with ␣ -sm actin both by Western blotting and immunohistochemistry. Using lung as a model organ system, we examined the developmental appearance of ␣ -sm Tm in comparison to ␣ -sm actin in both the mouse and human. ␣ -sm Tm is a late-onset protein, appearing much later than actin in both species. There were some differences in onset of appearance in vascular and airway smooth muscle with airway appearing earlier. ␣ -sm Tm can therefore be used as a good marker of mature differentiated smooth muscle cells. Along with ␣ -sm actin and sm-myosin antibodies, ␣ -sm Tm is a valuable tool for the study of smooth muscle.

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Section: Discussionmentioning

confidence: 99%

Smooth Muscle-specific α Tropomyosin Is a Marker of Fully Differentiated Smooth Muscle in Lung

Vrhovski

McKay

Schevzov

et al. 2005

J Histochem Cytochem.

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“…The LC24 was generated against bacterially produced human Tm4 with only the C-terminal end used. 62 This antibody detects both rat and human Tm4 but also 1b amino acids [29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44] 64 detects all the cytoskeletal products from this gene both human and mouse (Fig. 8E).…”

Section: 56mentioning

confidence: 99%

“…The TPM1 gene has been found by RT-PCR to code for 20 isoforms in rat adult tissues and cultured cells. 32 Included among the encoded isoforms is the αTm expressed in striated muscle, the recently discovered TPM1κ in human hearts, 35 the isoforms preferentially enriched in the central nervous system, TmBr1, TmBr2 and TmBr3 and Tm2, Tm3, Tm5a and Tm5b. The TPM2 gene codes for the skeletal muscle βTm isoform and, thus far, only one cytoskeletal isoform, Tm1, has been detected.…”

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confidence: 99%

Tropomyosin isoforms and reagents

Schevzov¹,

Whittaker²,

et al. 2011

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“…The complex as a whole is an important, naturalistic model system because taxa are characterized by extensive interspecific and intraspecific variation for a number of ecologically important traits, including expression of metamorphic vs. nonmetamorphic (paedomorphic) life histories (Gould 1977;Shaffer and Voss 1996), timing of metamorphosis (Rose and Armentrout 1976;Voss and Smith 2005), cannibal vs. normal larval morphologies (Powers 1907;Hoffman and Pfennig 1999), infectious disease (Collins et al 2004), variation in adult coloration and pigment patterning (Reese 1969;Parichy 1996Parichy , 1998, and variation in general morphology (Shaffer 1984;Irschick and Shaffer 1997). In addition, these salamanders are important laboratory models for olfaction (Marchand et al 2004;Park et al 2004), vision (Thoreson et al 2004;Chichilnisky and Reike 2005), cardiogenesis (Denz et al 2004;Zhang et al 2004), embryogenesis (Bachvarova et al 2004;Ericsson et al 2004), and postembryonic development (Parichy 1998;Voss and Smith 2005), including organ and tissue regeneration (Christensen et al 2002;Schnapp and Tanaka 2005). Both natural and laboratory-based research areas are in need of a comprehensive genome map that can be used to identify the position and effect of loci that contribute to phenotypic variation and that can be used to compare features of the salamander genome to other vertebrates.…”

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confidence: 99%

A Comprehensive Expressed Sequence Tag Linkage Map for Tiger Salamander and Mexican Axolotl: Enabling Gene Mapping and Comparative Genomics in Ambystoma

et al. 2005

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Expressed sequence tag (EST) markers were developed for Ambystoma tigrinum tigrinum (Eastern tiger salamander) and for A. mexicanum (Mexican axolotl) to generate the first comprehensive linkage map for these model amphibians. We identified 14 large linkage groups (125.5-836.7 cM) that presumably correspond to the 14 haploid chromosomes in the Ambystoma genome. The extent of genome coverage for these linkage groups is apparently high because the total map size (5251 cM) falls within the range of theoretical estimates and is consistent with independent empirical estimates. Unlike most vertebrate species, linkage map size in Ambystoma is not strongly correlated with chromosome arm number. Presumably, the large physical genome size (30 Gbp) is a major determinant of map size in Ambystoma. To demonstrate the utility of this resource, we mapped the position of two historically significant A. mexicanum mutants, white and melanoid, and also met, a quantitative trait locus (QTL) that contributes to variation in metamorphic timing. This new collection of EST-based PCR markers will better enable the Ambystoma system by facilitating development of new molecular probes, and the linkage map will allow comparative studies of this important vertebrate group. T HE tiger salamander (Ambystoma tigrinum) species complex consists of several closely related and phenotypically diverse taxa that range from central Mexico to southern Canada (Shaffer and McKnight 1996). The complex as a whole is an important, naturalistic model system because taxa are characterized by extensive interspecific and intraspecific variation for a number of ecologically important traits, including expression of metamorphic vs.

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Expression of a novel cardiac-specific tropomyosin isoform in humans

Cited by 64 publications

References 13 publications

Smooth Muscle-specific α Tropomyosin Is a Marker of Fully Differentiated Smooth Muscle in Lung

Smooth Muscle-specific α Tropomyosin Is a Marker of Fully Differentiated Smooth Muscle in Lung

Tropomyosin isoforms and reagents

A Comprehensive Expressed Sequence Tag Linkage Map for Tiger Salamander and Mexican Axolotl: Enabling Gene Mapping and Comparative Genomics in Ambystoma

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