Expression of a Peppermint (E)-β-Farnesene Synthase Gene in Rice Has Significant Repelling Effect on Bird Cherry-Oat Aphid (Rhopalosiphum padi)

Gao, Liang; Zhang, Xiting; Zhou, Fei; Chen, Hao

doi:10.1007/s11105-015-0888-4

Cited by 15 publications

(16 citation statements)

References 32 publications

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“…The coupled GC–MS analysis and behavior results showed that the transgenic alfalfa emitted EβF, and repelled pea aphids under laboratory conditions. Similar results have been observed with peach aphids M. persicae to Arabidopsis and Nicotiana tabacum (tobacco), and bird cherry‐oat aphid Rhopalosiphum padi to Oryza sativa (rice) . These results suggested that EβF synthase gene can more functionally increase pea aphid aversion to transgenic alfalfa, with the targeted gene approach offering a feasible option to reduce aphid infestations in alfalfa.…”

Section: Discussionsupporting

confidence: 80%

(E)‐β‐farnesene synthase gene affects aphid behavior in transgenic Medicago sativa

Wang

Gao

Chen

et al. 2018

Pest Management Science

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Section: Discussionsupporting

confidence: 80%

(E)‐β‐farnesene synthase gene affects aphid behavior in transgenic Medicago sativa

Wang

Gao

Chen

et al. 2018

Pest Management Science

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“…There is a medium-sized gene family for most plant genomes with encoding terpene synthetases. It is important for studying the physiological and 4 ecological functions of the specific terpenoids by using the plants that overexpress the TPS gene [15,[23][24][25][26][27]. Over expression of the wheat FPP synthase gene (FPS) in Arabidopsis could repel the Myzus persicae Sulzer [26].…”

Section: Introductionmentioning

confidence: 99%

WITHDRAWN: De novo assembly and comparative transcriptome analysis revealed the genes that were potentially involved in defensive terpenoids emission in the wheat against the wheat aphid Sitobion avenae (Fabricius)

Zhao

Liu

Zhan

et al. 2019

Preprint

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Background Terpenoid volatiles play an important role directly or indirectly in the plant defense mechanisms against herbivores, including the gramineous crops such as transgenic rice and corn. The conventional varieties of wheat are important gramineous cereal crops that lack aphid-resistant genes. Therefore, it is necessary to seek the aphid-resistant genes by screening for potential terpenoid synthase genes in the wheat germplasm resources. Results The result showed that aphid-damaged Octoploid Tirtitrigia emitted a higher amount of S-linalool, ent-kaurene, (+)-delta-cadinene, (3S,6E)-nerolidol as compared to the intact plant. In addition, (E)-β-caryophyllene, β‐Myrcene, (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT) were new volatile terpenoids emitted by the damaged plant. Further olfactory responses tests showed that S-linalool significantly repelled Sitobion avenae (Fabricius). After de novo assembly and quantitative assessment, a total of 182348 (74.8%) unigenes were annotated by alignment with the public protein databases. Of these unigenes, 2389 differentially expressed genes were identified between the intact and damaged ears of Octoploid Trititrigia. The expression profile of 10 randomly selected TPSs was confirmed with RT-qPCR. Candidate genes involved in terpenes biosynthesis were identified by showing the significant transcript changes between the intact and damaged plant ears of Octoploid Trititrigia. The transcript abundances of terpenes biosynthetic pathway-related genes were also positively correlated with the production of volatile terpenoids in the ears. The unigenes of S-linalool synthase gene was mapped to the cloned cDNA WT008_M07 (AK333728) and WT013_P07 (AK335856) of the Chinese spring wheat cultivar. The predicted protein complete ORF sequence (TaLIS1/2) when compared with the S-linalool synthase gene of other species, contained an aspartate-rich region DDxxD motif. Its function was characterized as coordinating the divalent metal ions involved in substrate binding. Furthermore, the phylogenetic tree results indicated that the TaLIS1 and TuNES1 are highly homologous. Conclusions This assembled transcriptome of S. avenae-damaged Octoploid Trititrigia and the intact ears could provide more molecular resources for the future functional characterization analysis of genomics in volatile terpenoids involved in direct or indirect defenses. Our study describes the metabolic regulation mechanism of the volatile terpenoids in the gramineous crops, which provides support for both breeding and genetic modification of the wheat varieties resistant to wheat aphid.

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“…The generation of genetically modified plants with overexpressed TPS genes is useful in studying the physiological and ecological functions of specific terpenoids (Aharoni et al ; Degenhardt et al ; Brillada et al ; Gao et al ; Zhang et al ). For example, targeting of the TPS gene FaNES1 of strawberry Fragaria × ananassa, into A. thaliana mitochondria resulted in the recruitment of carnivorous predatory mites through the enhanced production of ( E )‐nerolidol and DMNT (Kappers et al ).…”

Section: Introductionmentioning

confidence: 99%

Expression of lima bean terpene synthases in rice enhances recruitment of a beneficial enemy of a major rice pest

Lin

et al. 2017

Plant Cell & Environment

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Volatile terpenoids play a key role in plant defence against herbivory by attracting parasitic wasps. We identified seven terpene synthase genes from lima bean, Phaseolus lunatus L. following treatment with either the elicitor alamethicin or spider mites, Tetranychus cinnabarinus. Four of the genes (Pltps2, Pltps3, Pltps4 and Pltps5) were up-regulated with their derived proteins phylogenetically clustered in the TPS-g subfamily and PlTPS3 positioned at the base of this cluster. Recombinant PlTPS3 was able to convert geranyl diphosphate and farnesyl diphosphate to linalool and (E)-nerolidol, the latter being precursor of the homoterpene (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT). Recombinant PlTPS4 showed a different substrate specificity and produced linalool and (E)-nerolidol, as well as (E,E)-geranyllinalool from geranylgeranyl diphosphate. Transgenic rice expressing Pltps3 emitted significantly more (S)-linalool and DMNT than wild-type plants, whereas transgenic rice expressing Pltps4 produced (S)-linalool, DMNT and (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT). In laboratory bioassays, female Cotesia chilonis, the natural enemy of the striped rice stemborer, Chilo suppressalis, were significantly attracted to the transgenic plants and their volatiles. We further confirmed this with synthetic blends mimicking natural rice volatile composition. Our study demonstrates that the transformation of rice to produce volatile terpenoids has the potential to enhance plant indirect defence through natural enemy recruitment.

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Expression of a Peppermint (E)-β-Farnesene Synthase Gene in Rice Has Significant Repelling Effect on Bird Cherry-Oat Aphid (Rhopalosiphum padi)

Cited by 15 publications

References 32 publications

(E)‐β‐farnesene synthase gene affects aphid behavior in transgenic Medicago sativa

(E)‐β‐farnesene synthase gene affects aphid behavior in transgenic Medicago sativa

WITHDRAWN: De novo assembly and comparative transcriptome analysis revealed the genes that were potentially involved in defensive terpenoids emission in the wheat against the wheat aphid Sitobion avenae (Fabricius)

Expression of lima bean terpene synthases in rice enhances recruitment of a beneficial enemy of a major rice pest

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