Expression of a single transfected cDNA converts fibroblasts to myoblasts

Davis, Robert L.; Weintraub, Harold; Lassar, Andrew B.

doi:10.1016/0092-8674(87)90585-x

Cited by 3,189 publications

(2,223 citation statements)

References 45 publications

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“…The fibroblasts transduced with MyoD were converted to myoblasts in vitro. 21) Overexpression of C/EBP induced the direct conversion from B cells to macrophages. 22) By suppressing Pax5, B cells can dedifferentiate into progenitor cells, which can differentiate into multiple hematopoietic lineages.…”

Section: Cell Fate Conversion By Defined Factorsmentioning

confidence: 99%

Induction of pluripotency by defined factors

Okita

Yamanaka

2010

Experimental Cell Research

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Abstract:The "reversion of cell fate from differentiated states back into totipotent or pluripotent states" has been an interest of many scientists for a long time. With the help of knowledge accumulated by those scientists, we succeeded in converting somatic cells to a pluripotent cell lineage by the forced expression of defined factors. These established induced pluripotent stem (iPS) cells have similar features to embryonic stem (ES) cells, including pluripotency and immortality. The iPS cell technology provides unprecedented opportunities for regenerative medicine and drug discovery.

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Section: Cell Fate Conversion By Defined Factorsmentioning

confidence: 99%

Induction of pluripotency by defined factors

Okita

Yamanaka

2010

Experimental Cell Research

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“…DIG in situ hybridization to mouse tissue sections was carried out as described by SchaerenWiemers and Gerfin-Moser (1993) with a 5 min proteinase K treatment. The mouse MyoD and capsulin cDNAs are as described in Davis et al (1987) and Kelly et al (2004), respectively.…”

Section: Rna In Situ Hybridizationmentioning

confidence: 99%

Tbx1 regulation of myogenic differentiation in the limb and cranial mesoderm

Dastjerdi

Robson

Walker

et al. 2006

Developmental Dynamics

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The T-box transcription factor Tbx1 has been implicated in DiGeorge syndrome, the most frequent syndrome due to a chromosomal deletion. Gene inactivation of Tbx1 in mice results in craniofacial and branchial arch defects, including myogenic defects in the first and second branchial arches. A T-box binding site has been identified in the Xenopus Myf5 promoter, and in other species, T-box genes have been implicated in myogenic fate. Here we analyze Tbx1 expression in the developing chick embryo relating its expression to the onset of myogenic differentiation and cellular fate within the craniofacial mesoderm. We show that Tbx1 is expressed before capsulin, the first known marker of branchial arch 1 and 2 muscles. We also show that, as in the mouse, Tbx1 is expressed in endothelial cells, another mesodermal derivative, and, therefore, Tbx1 alone cannot specify the myogenic lineage. In addition, Tbx1 expression was identified in both chick and mouse limb myogenic cells, initially being restricted to the dorsal muscle mass, but in contrast, to the head, here Tbx1 is expressed after the onset of myogenic commitment. Functional studies revealed that loss of Tbx1 function reduces the number of myocytes in the head and limb, whereas increasing Tbx1 activity has the converse effect. Finally, analysis of the Tbx1-mesoderm-specific knockout mouse demonstrated the cell autonomous requirement for Tbx1 during myocyte development in the cranial mesoderm. Developmental Dynamics 236:353-363, 2007.

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“…Filters were prehybridized and hybridized in a mixture containing 50% formamide, 5 mM NaPO 4 , 0.75 M NaCl, 1 mM EDTA, 0.5% sodium dodecyl sulfate (SDS), 0.4 mg/ml denaturated DNA salmon sperm, 106Denhardt solution, 1% dextran sulfate and the appropriated probe (10 6 c.p.m./ml) at 428C and washed twice (30 min each) in 0.26 standard saline citrate (SSC)/0.1% SDS at 658C. Filters were hybridized using the following cDNA probes labeled by random priming: mouse RAR a, b and RXR a, b (Zelent et al, 1989), mouse MyoD (Davis et al, 1987), mouse myogenin (Edmondson et al, 1989) and hamster ribosomal S26 protein . Radioactivity on the nylon membranes was determined on a PhosphorImager analyser.…”

Section: Rna Extraction and Northern Blot Analysismentioning

confidence: 99%