Expression of a sweet cherry DREB1/CBF ortholog in Arabidopsis confers salt and freezing tolerance

Kitashiba, Hiroyasu; Ishizaka, Takayuki; Isuzugawa, Kanji; Nishimura, Kenichi; Suzuki, Takashi

doi:10.1016/j.jplph.2004.04.008

Cited by 78 publications

(50 citation statements)

References 17 publications

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“…S4), implying that activation of the CBF regulon genes has resulted in the increased FT under nonacclimating conditions. This is in accordance with previous studies in Arabidopsis overexpressing Arabidopsis CBFs (Fowler and Thomashow, 2002;Seki et al, 2002) and with studies by Zhang et al (2004) and Kitashiba et al (2004), who showed that ectopic expression of functional CBF orthologs of tomato and sweet cherry, respectively, increased Arabidopsis stress tolerance. Taken together, the phenotypes of the transgenic Arabidopsis lines overexpressing birch CBF genes suggest that the studied birch genes indeed are orthologs of Arabidopsis CBF genes.…”

Section: Cbf Transcription Factors In Birchsupporting

confidence: 93%

“…Despite the increasing number of reports characterizing the cold acclimation process of woody plants at the molecular level, the mechanism for the extreme hardiness during winter is still largely unknown. Our previous results indicated that the CBF pathway is also operational in trees (Puhakainen et al, 2004), and indeed, the presence of putative CBF orthologs was recently described in various woody plants, for example, in sweet cherry (Prunus avium; Kitashiba et al, 2004), poplar (Populus spp. ; Benedict et al, 2006), Eucalyptus gunnii (El Kayal et al, 2006), grape (Vitis; Xiao et al, 2006), and Citrus (Champ et al, 2007).…”

mentioning

confidence: 59%

“…Although CBF/DREB1 genes are exclusively cold inducible, they are found both in species that can cold acclimate and in plants that do not tolerate freezing, like tomato (Jaglo et al, 2001). CBF transcription factors have also been described in trees (Kitashiba et al, 2004, Benedict et al, 2006, El Kayal et al, 2006Xiao et al, 2006;Champ et al, 2007), whose cold acclimation capacity exceeds that of any other plant species (for review, see Welling and Palva, 2006). Earlier studies have suggested an important role for CBF transcription factors also in the regulation of cold acclimation in response to LT in woody plants during the growing season.…”

Section: Discussionmentioning

confidence: 99%

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Involvement of CBF Transcription Factors in Winter Hardiness in Birch

2008

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Cold acclimation of plants involves extensive reprogramming of gene expression. In Arabidopsis (Arabidopsis thaliana), three cold-inducible transcriptional activators designated CBF1 to -3/DREB1a to -c have been shown to play an important regulatory role in this acclimation process. Similarly to Arabidopsis, boreal zone trees can increase their freezing tolerance (FT) in response to low temperature during the growing season. However, maximal FT of these trees requires short daylength-induced dormancy development followed by exposure to both low and freezing temperatures. To elucidate the molecular basis of FT in overwintering trees, we characterized the role of birch (Betula pendula) CBF transcription factors in the cold acclimation process. We identified four putative CBF orthologs in a birch expressed sequence tag collection designated BpCBF1 to -4. Ectopic expression of birch CBFs in Arabidopsis resulted in constitutive expression of endogenous CBF target genes and increased FT of nonacclimated transgenic plants. In addition, these plants showed stunted growth and delayed flowering, typical features for CBF-overexpressing plants. Expression analysis in birch showed that BpCBF1 to -4 are low temperature responsive but differentially regulated in dormant and growing plants, the expression being delayed in dormant tissues. Freeze-thaw treatment, simulating wintertime conditions in nature, resulted in strong induction of BpCBF genes during thawing, followed by induction of a CBF target gene, BpLTI36. These results suggest that in addition to their role in cold acclimation during the growing season, birch CBFs appear to contribute to control of winter hardiness in birch.

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Section: Cbf Transcription Factors In Birchsupporting

confidence: 93%

mentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

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Involvement of CBF Transcription Factors in Winter Hardiness in Birch

2008

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“…Although recent breakthroughs have increased the knowledge on the molecular basis of the CBF cold pathway in herbaceous species, little is known in woody plants. To date, data on CBF genes from perennial plants include functional analysis of CBF from cherry [12], eucalyptus [13], blueberry [11], and apple [10].…”

Section: Introductionmentioning

confidence: 99%

Isolation and expression analysis of CBF4 from <i>Vitis amurensis</i> associated with stress

Chen¹,

Zhang²,

Yu³

et al. 2013

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The C-repeat Binding Factor/Dehydration Responsive Element Binding factor (CBF/DREB) responsive pathway is involved in plant response to abiotic stress. In this study, we report the isolation of VaCBF4 (a complete cDNA) and its promoter from Vitis amurensis through rapid amplification of cDNA ends and genome walking techniques, respectively. The CBF4 transcript accumulation of V. amurensis increased under cold, salinity, and abscisic acid (ABA) and salicylic acid (SA) treatments, whereas that of Vitis vinifera showed a different change. The transcript levels of VaCBF4 in the roots, stems, leaves, and petioles under cold, salinity, and ABA and SA treatments were up-regulated in a timedependent manner. The presence of the cis-elements MBC, ABRE, and as-2-box in the VaCBF4 promoter further confirmed that this promoter is a component of the CBF transduction pathway, which is involved in plant response to multistress.

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“…In addition, similar results were observed in transgenic Brassica napus (Jaglo et al 2001) and maize (Qin et al 2004) by the overexpression of their respective DREB1/CBF ortholog genes. We have previously reported on the identification of three DREB1/CBF homologs (designated as CIG-A, -B and -C ) from sweet cherry (Kitashiba et al 2002(Kitashiba et al , 2003, and shown increased salt and freezing tolerance in transgenic Arabidopsis by overexpression of CIG-B (Kitashiba et al 2004). In addition, nuclear localization signal (NLS) motif and some characteristic sequences such as Myb-/Myc-recoginition sites and G-box (E-box/ABRE-like motif) were found in the N-terminal region of CIG-B protein and in the 5Ј-upstream region of CIG-B (Kitashiba et al 2002).…”

mentioning

confidence: 99%

Nuclear localization of sweet cherry DREB1/CBF ortholog (CIG) and low temperature-inducible activity of CIG promoter

Kitashiba

Ban

Honda

et al. 2006

Plant Biotechnology

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The fluorescent signal of GFP fused to CIG-B, which is one of the three DREB1/CBF (dehydration responsive element binding protein 1/C-repeat binding factor) homologs isolated from sweet cherry, was observed only in nucleus. The expression of GFP driven by CIG-B promoter was up-regulated by low-temperature. These results suggested that nuclear localization signal (NLS) motif in the N-terminal region of CIG-B protein and inducer of CBF expression (ICE)-like motifs in CIG-B promoter could be responsible for nuclear targeting and low temperature perception, respectively.

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Expression of a sweet cherry DREB1/CBF ortholog in Arabidopsis confers salt and freezing tolerance

Cited by 78 publications

References 17 publications

Involvement of CBF Transcription Factors in Winter Hardiness in Birch

Involvement of CBF Transcription Factors in Winter Hardiness in Birch

Isolation and expression analysis of CBF4 from <i>Vitis amurensis</i> associated with stress

Nuclear localization of sweet cherry DREB1/CBF ortholog (CIG) and low temperature-inducible activity of CIG promoter

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Expression of a sweet cherry DREB1/CBF ortholog in Arabidopsis confers salt and freezing tolerance

Cited by 78 publications

References 17 publications

Involvement of CBF Transcription Factors in Winter Hardiness in Birch

Involvement of CBF Transcription Factors in Winter Hardiness in Birch

Isolation and expression analysis of CBF4 from &lt;i&gt;Vitis amurensis&lt;/i&gt; associated with stress

Nuclear localization of sweet cherry DREB1/CBF ortholog (CIG) and low temperature-inducible activity of CIG promoter

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Isolation and expression analysis of CBF4 from <i>Vitis amurensis</i> associated with stress