Expression of a truncated form of yeast ribosomal protein L3 in transgenic wheat improves resistance to Fusarium head blight

Di, Rong; Blechl, Ann E.; Dill‐Macky, Ruth; Tortora, Andrew; Tumer, Nilgun E.

doi:10.1016/j.plantsci.2010.02.003

Cited by 38 publications

(17 citation statements)

References 32 publications

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“…Furthermore, there has been a lack of wheat and barley genome sequences, although barley genome has recently been published (The International Barley Genome Sequencing Consortium, 2012), which should assist the process of identifying elements relating to resistance. Transgenic wheat expressing a α-1-purothionin, a thaumatin-like protein 1, a β-1,3-glucanase (MacKintosh et al, 2007), a class II chitinase (Shin et al, 2008), an antifungal plant defensin (Li et al, 2011), a pectin methyl-esterase inhibitor (Volpi et al, 2011), a polygalacturonase-inhibiting protein (Ferrari et al, 2012), a lactoferrin (Han et al, 2012), a Arabidopsis thaliana NPR1 (Makandar et al, 2006), or a truncated form of the yeast ribosomal protein L3 (Di et al, 2010) enhanced resistance to FHB under greenhouse conditions. However, transgenic lines have rarely been tested for FHB severity under field conditions.…”

Section: Contribution Of Proteomics To Crop Protection Against Fhbmentioning

confidence: 99%

Fusarium graminearum and Its Interactions with Cereal Heads: Studies in the Proteomics Era

et al. 2013

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The ascomycete fungal pathogen Fusarium graminearum (teleomorph stage: Gibberella zeae) is the causal agent of Fusarium head blight in wheat and barley. This disease leads to significant losses of crop yield, and especially quality through the contamination by diverse fungal mycotoxins, which constitute a significant threat to the health of humans and animals. In recent years, high-throughput proteomics, aiming at identifying a broad spectrum of proteins with a potential role in the pathogenicity and host resistance, has become a very useful tool in plant-fungus interaction research. In this review, we describe the progress in proteomics applications toward a better understanding of F. graminearum pathogenesis, virulence, and host defense mechanisms. The contribution of proteomics to the development of crop protection strategies against this pathogen is also discussed briefly.

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Section: Contribution Of Proteomics To Crop Protection Against Fhbmentioning

confidence: 99%

Fusarium graminearum and Its Interactions with Cereal Heads: Studies in the Proteomics Era

et al. 2013

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“…There is relatively little published work on increased fungal disease resistance of transgenic wheat in the field. In four reports, the transgenes conferred resistance to Fusarium head blight, one of them also to wheat sharp eyespot (Mackintosh et al, 2007;Shin et al, 2008;Di et al, 2010;Li et al, 2011). In addition, Tilletia caries (stinking smut)-resistant wheat lines were described (Schlaich et al, 2006).…”

Section: The Effective Use Of Pm3 Alleles In Transgenic Multilinesmentioning

confidence: 99%

TransgenicPm3multilines of wheat show increased powdery mildew resistance in the field

Brunner

Stirnweis

Quijano

et al. 2011

Plant Biotechnology Journal

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SummaryResistance (R) genes protect plants very effectively from disease, but many of them are rapidly overcome when present in widely grown cultivars. To overcome this lack of durability, strategies that increase host resistance diversity have been proposed. Among them is the use of multilines composed of near-isogenic lines (NILs) containing different disease resistance genes. In contrast to classical R-gene introgression by recurrent backcrossing, a transgenic approach allows the development of lines with identical genetic background, differing only in a single R gene. We have used alleles of the resistance locus Pm3 in wheat, conferring race-specific resistance to wheat powdery mildew (Blumeria graminis f. sp. tritici), to develop transgenic wheat lines overexpressing Pm3a, Pm3c, Pm3d, Pm3f or Pm3g.In field experiments, all tested transgenic lines were significantly more resistant than their respective nontransformed sister lines. The resistance level of the transgenic Pm3 lines was determined mainly by the frequency of virulence to the particular Pm3 allele in the powdery mildew population, Pm3 expression levels and most likely also allele-specific properties. We created six two-way multilines by mixing seeds of the parental line Bobwhite and transgenic Pm3a, Pm3b and Pm3d lines. The Pm3 multilines were more resistant than their components when tested in the field. This demonstrates that the difference in a single R gene is sufficient to cause host-diversity effects and that multilines of transgenic Pm3 wheat lines represent a promising strategy for an effective and sustainable use of Pm3 alleles.

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“…SG was also found to specifically interact with 40S and 60S ribosomal subunit as early as 5 minutes and led to polysome disaggregation in RAW 264.7 cells [10]. It has been suggested that trichothecenes targeted the ribosomal protein L3 (Rpl3) at the peptidyltransferase centre of 60S subunit, which led to protein synthesis inhibition [11,12]. Heterogenous expression of N-terminal fragment of yeast Rpl3 in tobacco conferred resistance to DON [13].…”

Section: Introductionmentioning

confidence: 99%

WITHDRAWN: Mitochondria: a critical target in the toxicity of trichothecenes and potential treatment strategies

Huang¹,

Cui²,

Wu³

et al. 2018

Oncotarget

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Copyright: Huang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ABSTRACTTrichothecenes are secondary metabolites produced by fungi of genus Fusarium. It was known that trichothecenes can bind to the 60S subunit of the eukaryotic ribosomes, resulting in inhibition of protein synthesis. However, emerging evidences suggest that trichothecenes can target the mitochondria to induce mitochondrial dysfunction and mitochondria-dependent apoptosis. Numerous studies have investigated the mitochondria-associated toxicities induced by trichothecenes. Here, we mainly collected the data associated with mitochondria and its relationship with trichothecene-induced toxic effects. We showed that mitochondria are the main site of reactive oxygen species production and mediate the trichothecene-induced apoptosis in various cells. The mitochondrial membrane permeabilization plays critical roles in this apoptotic process. Trichothecenes can penetrate the mitochondrial membrane due to its amphiphilic property, and inhibit the activities of mitochondrial respiratory chain complexes and electron transfer in mitochondria. Besides, trichothecenes reduce the mitochondrial biogenesis and mitochondrial transcription and translation. Peroxisome proliferator-activated receptor-γ co-activator 1α and mammalian target of rapamycin pathway are closely linked to this process. Trichothecenes can activate various microRNAs that may mediate the protein synthesis inhibition. These microRNAs may have a crosstalk with mitochondria to mediate the mitochondrial dysfunction and transcription repression in mitochondrial genome. Numerous natural compounds have been tested for their effective antioxidant and anti-inflammatorycapacities. Specially, mitochondria-targeted antioxidants and mitophagy exhibit outstanding cytoprotective abilitysince they can reduce the mitochondrial reactive oxygen species and maintain the mitochondrial quality. This review may shed some new light on the mitochondriaassociated mechanism underlying the toxicity of trichothecenes and new preventive ways to combat these mycotoxins. www.impactjournals.com/oncotarget/

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Expression of a truncated form of yeast ribosomal protein L3 in transgenic wheat improves resistance to Fusarium head blight

Cited by 38 publications

References 32 publications

Fusarium graminearum and Its Interactions with Cereal Heads: Studies in the Proteomics Era

Fusarium graminearum and Its Interactions with Cereal Heads: Studies in the Proteomics Era

TransgenicPm3multilines of wheat show increased powdery mildew resistance in the field

WITHDRAWN: Mitochondria: a critical target in the toxicity of trichothecenes and potential treatment strategies

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