Expression of a Variant Chitinase Transcript of Helicoverpa armigera in E. coli
Abstract:Gene encoding for chitinase was isolated and sequenced from the cotton bollworm Helicoverpa armigera, using the polymerase chain reaction technique directed by primers designed from the chitinase cDNA of the same insect reported by Ahmad et al. (2003). The nucleotide sequence analysis suggested that the gene is of 1737 bp showing homolgy of 96% with the reference chitinase sequence (AY325496) and 80% homology with that of Manduca sexta, the first cloned insect chitinase gene (U02270). The cDNA encoded a polype… Show more
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