Expression of a wheat cytochrome P450 monooxygenase cDNA in yeast catalyzes the metabolism of sulfonylurea herbicides

Xiang, Wensheng; Wang, Xiangjing; Ren, Tongjun

doi:10.1016/j.pestbp.2005.09.001

Cited by 65 publications

(59 citation statements)

References 30 publications

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“…Phenoxyalkanoic acid Aryl hydroxylation Tulip bulb microsomes (Topal et al 1993) NADPH dependence; inhibition by CO; metabolism by purified, reconstituted enzyme; spectral evidence Chlortoluron Phenylurea N-demethylation and/or arylalkyl hydroxylation Jerusalem artichoke cDNAs (CabelloHurtado et al 1998;Didierjean et al 2002;Pierrel et al 1994;Robineau et al 1998) Heterologous expression of CYP73A1and CYP81B1 in yeast or CYP76B1 in yeast and tobacco enhances herbicide metabolism Soybean cDNA (Siminszky et al 1999) Heterologous expression of CYP71A10 in yeast and tobacco enhances herbicide metabolism Tobacco cDNA (Yamada et al 2000) Heterologous expression of CYP71A11 and CYP81B2 in yeast enhances herbicide metabolism Wheat microsomes (Mougin et al 1990;Mougin et al 1992;Mougin et al 1991;Zimmerlin and Durst 1992 (Xiang et al 2006) heterologous expression of CYP71C6v1 in yeast enhances herbicide metabolism chemical. P450-catalyzed metabolism of a herbicide will not result in enhanced herbicide resistance if the phytotoxicity of reaction product(s) is equal or greater than that of the parent molecule.…”

Section: -4dmentioning

confidence: 99%

“…CYP71C6v1, a wheat P450 cDNA, was isolated from seedling tissues by reverse-transcription-PCR through the random amplification of CYP71C subfamily members using CYP71C1-specific primers. (Xiang et al 2006). When expressed in yeast, CYP71C6v1 displayed chlorsulfuron and triasulfuron 5-hydroxylase activity and also catalyzed the metabolism of metsulfuron-methyl, bensulfuronmethyl, and tribenuron-methyl to yet unidentified products (Xiang et al 2006).…”

Section: Few P450 Genes Involved In Herbicide Metabolism Have Been Ismentioning

confidence: 99%

“…(Xiang et al 2006). When expressed in yeast, CYP71C6v1 displayed chlorsulfuron and triasulfuron 5-hydroxylase activity and also catalyzed the metabolism of metsulfuron-methyl, bensulfuronmethyl, and tribenuron-methyl to yet unidentified products (Xiang et al 2006).…”

Section: Few P450 Genes Involved In Herbicide Metabolism Have Been Ismentioning

confidence: 99%

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Plant cytochrome P450-mediated herbicide metabolism

2006

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In the last two decades it has become apparent that enzymes of the P450 monooxygenase (P450) superfamily are responsible for the Phase I metabolism of numerous herbicides representing several classes of organic compounds. The majority of experimental evidence for P450 involvement in herbicide metabolism has been derived from in vitro studies in which the catalytic activity of plant microsomes towards herbicidal substrates was measured in the presence of various P450 inhibitors and activators. While the studies with microsomes elicited much appreciation for the pivotal roles of plant P450s in herbicide metabolism, detailed characterization of these enzymes only became possible after the isolation of genes encoding specific isoforms responsible for herbicide conversion. Several lines of evidence suggest that the development of herbicide resistance in weeds by enhanced detoxification is frequently associated with elevated levels of P450 activity. Enhanced detoxification-based herbicide resistance is particularly difficult to control, because it can involve resistance to multiple, chemically unrelated classes of herbicides. Continued research efforts are aimed at elucidating the role of P450s in the metabolic fates of herbicides in plants and the development of herbicide resistance in weeds. Recent advances made in the isolation and genetic manipulation of P450 enzymes have created new opportunities for their application in engineering herbicide tolerance and bioremediation.

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Section: -4dmentioning

confidence: 99%

Section: Few P450 Genes Involved In Herbicide Metabolism Have Been Ismentioning

confidence: 99%

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Plant cytochrome P450-mediated herbicide metabolism

2006

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“…In addition to being responsible for the resistance in weeds, genes CytP450 are also related to the herbicides tolerance mechanisms in crops, such as CYP72A21 and CYP81A6 in O. sativa Pan et al, 2006;Hirose et al, 2007), CYP72A5 in Zea mays (Persans et al, 2001), CYP81B2 and CYP71A11 in Nicotiana tabaccum (Yamada et al, 2000), CYP71C6v1 in Triticum aestivum (Xiang et al, 2006) and CYP71A10 in Glycine max (Siminszky et al, 1999).…”

Section: Enzymes Involved In the Herbicide Degradationmentioning

confidence: 99%

Ocorrência e importância da resistência a herbicidas causada por incremento de metabolização para o manejo de plantas daninhas

Dalazen

Markus²,

Kaspary³

et al. 2016

RBHerbicidas

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-Non-target-site resistance (NTSR) to herbicides mainly caused by enhanced degradation is highly problematic due to the occurrence of biotypes with multiple resistance and is a new challenge for weed management and herbicide use. Recently, enzymes associated with xenobiotic degradation especially cytochrome P450, GSTs and ABC transporters have been associated with the herbicide resistance in several weeds. This knowledge opens a new window to understand the evolution of NTSR. The aims of this review are to discuss the current knowledge of the gene regulation associated with the herbicide resistance caused by enhanced herbicide degradation and to analyze the main consequences of this problem for the adequate herbicide use and weed management. Multiple herbicide resistance caused by degradation enhancement occurs in Lolium rigidum, Alopecurus myosuroides, Echinochloa phyllopogon and in several other species. The level of herbicide resistance caused by degradation enhancement is affected by environmental factors in several cases, which difficult the resistance diagnostic and facilitate its distribution. The modern weed management should consider the characteristics of the herbicide degradation, since the simple rotation of herbicides mechanism of action might not be enough to prevent the herbicide resistance. The use of synergistic mixtures of herbicides and other enzyme inhibitors may contribute to prevent the evolution and spreading of NTSR weed herbicide resistance. Keywords: multiple resistance; cytochrome P450; detoxification; NTSR Resumo -A resistência a herbicidas causada por mecanismos não relacionados ao local de ação (NRLA), principalmente causada pelo incremento de metabolização, é altamente problemática devido à ocorrência de biótipos com resistência múltipla, e é um novo desafio para o manejo de plantas daninhas e utilização de herbicidas. Recentemente, enzimas associadas à degradação de xenobióticos, especialmente citocromo P450, GSTs e transportadores ABC, têm sido associadas à resistência a herbicidas em várias plantas daninhas. Os objetivos desta revisão são descrever o conhecimento atual da regulação gênica associada à resistência a herbicidas causada pelo incremento de metabolização e analisar as principais implicações deste problema para definir estratégias adequadas de manejo com o uso de herbicidas. A resistência múltipla a herbicidas causada pelo incremento da degradação ocorre em Lolium rigidum, Alopecurus myosuroides, Echinochloa phyllopogon e outras várias espécies. O nível de resistência a herbicidas causada pelo incremento de metabolização é afetado por fatores ambientais em vários casos, o que dificulta o diagnóstico da resistência e facilita sua distribuição. O manejo moderno de plantas daninhas deve considerar as características de degradação do herbicida, uma vez que a simples rotação de mecanismo de ação de herbicidas pode não ser suficiente para prevenir a ocorrência da resistência a estes produtos. A utilização de misturas sinergísticas de herbicidas e outros inibidores e...

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“…In wheat, the CYP71C6V1 was able to metabolize sulfonylurea herbicides such as triasulfuron, chlorsulfuron 15 . However, the tolerance to the chlortoluron has not been reported to date.…”

Section: Research Articlementioning

confidence: 99%

Mechanism of the plant cytochrome P450 for herbicide resistance: a modelling study

Fang

et al. 2012

Journal of Enzyme Inhibition and Medicinal Chemistry

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Expression of a wheat cytochrome P450 monooxygenase cDNA in yeast catalyzes the metabolism of sulfonylurea herbicides

Cited by 65 publications

References 30 publications

Plant cytochrome P450-mediated herbicide metabolism

Plant cytochrome P450-mediated herbicide metabolism

Ocorrência e importância da resistência a herbicidas causada por incremento de metabolização para o manejo de plantas daninhas

Mechanism of the plant cytochrome P450 for herbicide resistance: a modelling study

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