Expression of a zebrafish caudal homeobox gene correlates with the establishment of posterior cell lineages at gastrulation

Joly, Jean‐Stéphane; Maury, Martine; Joly, Claire; Duprey, Philippe; Boulekbache, Habib; Condamine, Hubert

doi:10.1111/j.1432-0436.1992.tb00488.x

Cited by 83 publications

(38 citation statements)

References 43 publications

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“…2R). As in the various bmp mutants, this reduced ventral specification is accompanied by impaired posterior development, as anticipated by reduced expression of the posterior marker cdx1 (Joly et al, 1992) on the ventral side of admp morphant embryos (Fig. 2S).…”

Section: Resultsmentioning

confidence: 99%

Zebrafish admp is required to restrict the size of the organizer and to promote posterior and ventral development

Lele

Nowak

Hammerschmidt

2001

Developmental Dynamics

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Bone morphogenetic proteins (Bmps) and their roles during early dorsoventral patterning of the vertebrate embryo are well understood. The role and regulation of a more distant member of this family, the anti-dorsalizing morphogenetic protein (Admp), however, are less clear. Here, we report the isolation and characterization of zebrafish admp. Unlike other bmps, admp is exclusively expressed on the dorsal side. Expression starts at blastula stages in the region of the organizer, giving rise to anterior neuroectoderm and axial mesoderm. During the course of gastrulation, both the neuroectodermal and the mesodermal admp transcripts vanish in an anterior-posterior wave. The maintenance of admp expression is positively influenced by Nodal signaling and by Bozozok (Boz), an organizer-promoting homeodomain protein acting as a repressor of early bmp2b expression. Despite the positive effect of boz on admp expression, Boz and Admp have rather opposite effects on zebrafish patterning, as revealed in gain-and loss-of-function experiments. Upon overexpression, admp has Bmp-like activities causing a smaller organizer and enhanced ventral specification, very similar to the phenotype caused by the loss of boz function in mutant embryos. Antisense-based admp knockdown, on the other side, leads to an enlarged organizer and impaired ventral and posterior development, as observed in embryos after boz overexpression. This finding indicates that admp is required for the development of embryonic structures normally suppressed by organizer activities. The seeming discrepancy between the regulative and functional relationship of boz and admp is discussed, and models are proposed according to which Admp might be part of a negative feedback loop to pattern and confine the organizer region.

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Section: Resultsmentioning

confidence: 99%

Zebrafish admp is required to restrict the size of the organizer and to promote posterior and ventral development

Lele

Nowak

Hammerschmidt

2001

Developmental Dynamics

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“…cdx4 and pdx1 are expressed in overlapping gradients Vertebrates express Cdx4 in all three germ layers during development (Davidson et al, 2003;Gamer and Wright, 1993;Joly et al, 1992). In zebrafish, the presumptive endoderm begins to express cdx4 during late epiboly (Joly et al, 1992), but details of its subsequent endodermal expression pattern are lacking.…”

Section: Resultsmentioning

confidence: 99%

“…In situ probes were used as previously described (Prince et al, 1998), using probes for cdx4 (Joly et al, 1992), cebpa (Lyons et al, 2001), glucagon and somatostatin 2 (Argenton et al, 1999), somatostatin 1 (Devos et al, 2002), insulin (Milewski et al, 1998), islet1 (Inoue et al, 1994), trypsin (Biemar et al, 2001), pdx1 (Lin et al, 2004) and krox20 (also known as egr2 -ZFIN) (Oxtoby and Jowett, 1993). In situs for pax9 were performed with modifications as previously described (Jackman et al, 2004).…”

Section: In Situ Hybridization Immunohistochemistry and Imagingmentioning

confidence: 99%

Cdx4 is required in the endoderm to localize the pancreas and limitβ-cell number

et al. 2008

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Cdx transcription factors have crucial roles in anteroposterior patterning of the nervous system and mesoderm. Here we focus on the role of cdx4 in patterning the endoderm in zebrafish. We show that cdx4 has roles in determining pancreatic ␤-cell number, directing midline convergence of ␤-cells during early pancreatic islet formation, and specifying the anteroposterior location of foregut organs. Embryos deficient in cdx4 have a posteriorly shifted pancreas, liver and small intestine. The phenotype is more severe with knockdown of an additional Cdx factor, cdx1a. We show that cdx4 functions within the endoderm to localize the pancreas. Morpholino knockdown of cdx4 specifically in the endoderm recapitulates the posteriorly shifted pancreas observed in cdx4 mutants. Conversely, overexpression of cdx4 specifically in the endoderm is sufficient to shift the pancreas anteriorly. Together, these results suggest a model in which cdx4 confers posterior identity to the endoderm. Cdx4 might function to block pancreatic identity by preventing retinoic acid (RA) signal transduction in posterior endoderm. In support of this, we demonstrate that in cdx4-deficient embryos treated with RA, ectopic ␤-cells are located well posterior to the normal pancreatic domain.

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“…They reached maximal amounts at stage 13 (early neurula) and decreased to low levels after stage 15 (late neurula). Joly et al (1992) isolated and characterised a caudal type cDNA in zebrafish by screening late gastrula embryos with a C&-1 homeobox probe. The putative protein is similar to chick CdxA, appears just before the onset of gastrulation, and becomes undetectable 48 hr after fertilisation.…”

Section: Introductionmentioning

confidence: 99%

Expression of Cdx‐2 in the mouse embryo and placenta: Possible role in patterning of the extra‐embryonic membranes

Beck

Erler

Russell

et al. 1995

Developmental Dynamics

374

261

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Three mouse homologues of the Drosophila homeotic gene Caudal (Cad) have been described. They are currently designated Cdx-1, Cdz-2, and Cdz-4. Cdx-1 and 2 are both strongly expressed in the adult mid-and hindgut, while Cdx-1 and 4 have been shown to be activated in the embryonic primitive streak. Using a polyclonal antibody against a fusion protein containing the amino terminal 109 amino acids of murine Cdx-2, we here describe the topographical location of the gene product from early cleavage to 12.5 days of embryonic development. Cdx-2 expression begins at 3.5 days and is confined to the trophectoderm, being absent from the inner cell mass. Subsequently, staining is located in the extra-embryonic ectoderm adjacent to the epiblast, but sparing the more superficially placed polar, as well as the mural trophoblastic cells. Continuing expression in the fetal membranes involves the chorion, the allantoic bud, and, at even later stages, the spongiotrophoblast. From 8.5 days, Cdx-2 begins to be expressed in embryonic tissues, principally (unlike Cdx-1) in the posterior part of the gut from its earliest formation, as well as in the tail bud and in the caudal part of the neural tube. Cdz-2 is, therefore, transcribed well before any other membrane of the Cad homologue group and of the related H a -C group; its expression in the extra-embryonic membranes and in the hindgut reflects the phylogenetic relationship between the cloaca and the chorio-allantois and suggests the possibility that homeobox genes may be involved in placental development andlor patterning. o 1995 Wiley-Liss, Inc.

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Expression of a zebrafish caudal homeobox gene correlates with the establishment of posterior cell lineages at gastrulation

Cited by 83 publications

References 43 publications

Zebrafish admp is required to restrict the size of the organizer and to promote posterior and ventral development

Zebrafish admp is required to restrict the size of the organizer and to promote posterior and ventral development

Cdx4 is required in the endoderm to localize the pancreas and limitβ-cell number

Expression of Cdx‐2 in the mouse embryo and placenta: Possible role in patterning of the extra‐embryonic membranes

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