Expression of adiponectin receptors 1 (AdipoR1) and 2 (AdipoR2) in the porcine pituitary during the oestrous cycle

Kieżun, Marta; Maleszka, Anna; Smolińska, Nina; Nitkiewicz, Anna; Kamiński, Tadeusz

doi:10.1186/1477-7827-11-18

Cited by 34 publications

(29 citation statements)

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“…Lack of correlation between the protein and mRNA expression and enzyme activity may result from transcriptional, post-transcriptional or translation regulation, protein stability or enzyme activity as well as functioning feedbacks, i.e. high protein concentration may suppress mRNA expression or enzyme activity, and a high level of gene expression may diminish the post-transcriptional processes (Kiezun et al 2013. The death receptor-mediated pathway of apoptosis is activated when certain death receptor ligands of the tumour necrosis factor (TNF) family (such as FAS (1996) showed the ability of Fas for inducing granulosa cell apoptosis.…”

Section: Effect Of Resistin On Cell Apoptosismentioning

confidence: 99%

Resistin is a survival factor for porcine ovarian follicular cells

Rak¹,

Drwal²,

Wróbel³

et al. 2015

Reproduction

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Previously, we demonstrated the expression of resistin in the porcine ovary, the regulation of its expression and its direct effect on ovarian steroidogenesis. The objective of this study was to examine the effect of resistin on cell proliferation and apoptosis in a co-culture model of porcine granulosa and theca cells. First, we analysed the effect of resistin at 1 and 10 ng/ml alone or in combination with FSHand IGF1 on ovarian cell proliferation with an alamarBlue assay and protein expression of cyclins A and B using western blot. Next, the mRNA and protein expression of selected pro-apoptotic and pro-survival regulators of cell apoptosis, caspase-9, -8 and -3 activity and DNA fragmentation using real time PCR, western blot, fluorescent assay and an ELISA kit, respectively, were analysed after resistin treatment. Furthermore, we determined the effect of resistin on the protein expression of ERK1/2, Stat and Akt kinase. Using specific inhibitors of these kinases, we also checked caspase-3 activity and protein expression. We found that resistin, at both doses, has no effect on cell proliferation. The results showed that resistin decreased pro-apoptotic genes, which was confirmed on protein expression of selected factors. We demonstrate an inhibitory effect of resistin on caspase activity and DNA fragmentation. Finally, resistin stimulated phosphorylation of the ERK1/2, Stat and Akt and kinases inhibitors reversed resistin action on caspase-3 activity and protein expression to control. All of these results showed that resistin has an inhibitory effect on porcine ovarian cell apoptosis by activation of the MAPK/ERK, JAK/Stat and Akt/PI3 kinase signalling pathways. Reproduction (2015) 150 343-355

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Section: Effect Of Resistin On Cell Apoptosismentioning

confidence: 99%

Resistin is a survival factor for porcine ovarian follicular cells

Rak¹,

Drwal²,

Wróbel³

et al. 2015

Reproduction

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“…In addition, T-cadherin has been found to act as a receptor for hexameric and HMW forms of adiponectin (Hug et al 2004, Hui et al 2012. Specifically, AdipoR1 in pig tissues has been reported to be highly expressed (Kiezun et al 2013), which is the reason why we focused on this subtype of AdipoRs. In addition, only Adipor1 knockdown has been found to be able to prevent the acute effects of the globular form of adiponectin on rat cardiac metabolism and oxidation (Palanivel et al 2007).…”

Section: Discussionmentioning

confidence: 99%

Effect of monomeric adiponectin on cardiac function and perfusion in anesthetized pig

Grossini¹,

Prodam²,

Walker³

et al. 2014

Journal of Endocrinology

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Adiponectin, the most abundant adipokine released by adipose tissue, appears to play an important role in the regulation of vascular endothelial and cardiac function. To date, however, the physiological effects of human monomeric adiponectin on the coronary vasculature and myocardial systo-diastolic function, as well as on parasympathetic/sympathetic involvement and nitric oxide (NO) release, have not yet been investigated. Thus, we planned to determine the primary in vivo effects of human monomeric adiponectin on coronary blood flow and cardiac contractility/relaxation and the related role of autonomic nervous system, adiponectin receptors, and NO. In 30 anesthetized pigs, human monomeric adiponectin was infused into the left anterior descending coronary artery at constant heart rate and arterial blood pressure, and the effects on coronary blood flow, left ventricular systo-diastolic function, myocardial oxygen metabolism, and NO release were examined. The mechanisms of the observed hemodynamic responses were also analyzed by repeating the highest dose of human monomeric adiponectin infusion after autonomic nervous system and NO blockade, and after specific adiponectin 1 receptor antagonist administration. Intracoronary human monomeric adiponectin caused dose-related increases of coronary blood flow and cardiac function. Those effects were accompanied by increased coronary NO release and coronary adiponectin levels. Moreover, the vascular effects of the peptide were prevented by blockade of b 2 -adrenoceptors and NO synthase, whereas all effects of human monomeric adiponectin were prevented by adiponectin 1 receptor inhibitor. In conclusion, human monomeric adiponectin primarily increased coronary blood flow and cardiac systo-diastolic function through the involvement of specific receptors, b 2 -adrenoceptors, and NO release.

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“…In the case of granulosa and theca cells, total RNA was extracted using the TRIzol reagent. Other methodological details were in accordance with those used in the study of Kiezun et al (2013).…”

Section: Determination Of the Expression Of Adipor1 And Adipor2 Genesmentioning

confidence: 99%

“…Forward and reverse primers for GAPDH were used according to the paper of Nitkiewicz et al (2010). The conditions of the reaction were the same as in our previous study (Kiezun et al, 2013). The relative expression levels of AdipoR1 and AdipoR2 were calculated based on the comparative cycle threshold method (ΔΔCt) (Livak and Schmittgen, 2001) and normalised using the geometrical mean of the expression level of reference genes GAPDH and cyclophilin A.…”

Section: Determination Of the Expression Of Adipor1 And Adipor2 Genesmentioning

confidence: 99%

“…Western blotting analysis was performed as described by Smolinska et al (2007), using specific first and second antibodies which were presented in our previous study by Kiezun et al (2013). The results of Western blotting were quantified by densitometric scanning of immunoblots with GelScan for Windows version 1.45 software (Kucharczyk, Poland).…”

Section: Western Blottingmentioning

confidence: 99%

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Expression of adiponectin receptors 1 and 2 in the ovary and concentration of plasma adiponectin during the oestrous cycle of the pig

Maleszka

Smolińska

Nitkiewicz

et al. 2014

Acta Veterinaria Hungarica

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The aim of this study was to compare the expression levels of adiponectin receptor 1 and adiponectin receptor 2 mRNAs and proteins in porcine ovaries during four stages (days 2 to 3, 10 to 12, 14 to 16, 17 to 19) of the oestrous cycle and to measure adiponectin plasma concentrations during the same phases of the cycle. Higher mRNA expression of adiponectin receptor 1 was detected in porcine granulosa cells than in corpora lutea and theca cells (P < 0.01). In contrast, higher gene expression of adiponectin receptor 2 occurred in newly developed and mature corpora lutea (P < 0.01). The adiponectin receptor 1 protein content was the highest in corpora lutea isolated on days 2 to 3 of the cycle and was the lowest in theca interna cells (P < 0.01). The profile of adiponectin receptor 2 protein was similar to that of adiponectin receptor 1. Adiponectin plasma concentrations were significantly higher throughout the luteal phase than in the follicular phase (P < 0.01). In conclusion, the presence of adiponectin receptor 1 and adiponectin receptor 2 mRNAs and proteins in the porcine ovary suggests that adiponectin may directly affect ovarian functions through its own specific receptors. The expression of both receptors and adiponectin plasma concentration were dependent on hormonal status related to the stage of the cycle. Key words: Adiponectin receptors, adiponectin, pig, ovary, oestrous cycleAdiponectin is an adipose-secreted 244-amino-acid protein with 30 kDa molecular weight, which circulates in the blood in the form of a trimer (low molecular weight, LMW), a hexamer (middle molecular weight, MMW) or a multimer (high molecular weight, HMW) (Maeda et al., 1996). Adiponectin exerts its effect by binding to two distinct but structurally related 7-transmembrane receptors, adiponectin receptor 1 (AdipoR1) and adiponectin receptor 2 (AdipoR2).

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Expression of adiponectin receptors 1 (AdipoR1) and 2 (AdipoR2) in the porcine pituitary during the oestrous cycle

Cited by 34 publications

References 50 publications

Resistin is a survival factor for porcine ovarian follicular cells

Resistin is a survival factor for porcine ovarian follicular cells

Effect of monomeric adiponectin on cardiac function and perfusion in anesthetized pig

Expression of adiponectin receptors 1 and 2 in the ovary and concentration of plasma adiponectin during the oestrous cycle of the pig

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