Expression of Adrenergic Receptors in Bovine and Rabbit Oocytes and Preimplantation Embryos

Číkoš, Štefan; Czikková, S.; Chrenek, Peter; Makarevich, A. V.; Burkuš, Ján; Janštová, Žofia; Fabian, Denis; Koppel, Juraj

doi:10.1111/rda.12233

Cited by 15 publications

(18 citation statements)

References 37 publications

(36 reference statements)

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“…Osthole, gramine, and hordenine act as human b2-AR agonists b2-AR expression in skeletal muscle has previously been confirmed in several species. In fact, mouse and human b2-ARs share 88% sequence homology (20). To make sure that these four food factors also act as human b2-AR agonists, luciferase assays were performed using a human b2-AR expression plasmid.…”

Section: Genementioning

confidence: 99%

Identification of Functional Food Factors as β<sub>2</sub>-Adrenergic Receptor Agonists and Their Potential Roles in Skeletal Muscle

Chikazawa

Sato

2018

J Nutr Sci Vitaminol

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Maintaining skeletal muscle functions by controlling muscle metabolism is of utmost importance. β-Adrenergic receptor (β-AR), which is expressed in skeletal muscle, is a member of the G-protein-coupled receptor family that plays a critical role in the maintenance of muscle mass. In the present study, using luciferase reporter assays in β-AR-expressing HEK293 cells, we discovered several food factors that exhibited agonistic activity at mouse or human β-AR. Osthole, gramine, and hordenine were identified as both mouse and human β-AR agonists, whereas berberine was identified as a mouse β-AR agonist only. Additionally, intramuscular injection of gramine or hordenine in mice facilitated gene expression of several cAMP response element binding protein targets, which is thought to result in increased skeletal muscle protein synthesis. This study provides evidence that several food factors might exert potential health effects on skeletal muscle by enhancing cAMP signaling through the activation of β-AR.

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Section: Genementioning

confidence: 99%

Identification of Functional Food Factors as β<sub>2</sub>-Adrenergic Receptor Agonists and Their Potential Roles in Skeletal Muscle

Chikazawa

Sato

2018

J Nutr Sci Vitaminol

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“…There are data indicating that glucocorticoids and catecholamines may directly influence mammalian preimplantation embryos by binding to their respective receptors [ 5 , 6 , 7 ]. In addition, several animal studies have shown that glucocorticoid and catecholamine administration in vitro or in vivo can negatively affect developmental capacity and quality of preimplantation embryos [ 6 , 8 , 9 , 10 , 11 , 12 , 13 ].…”

mentioning

confidence: 99%

Stress exposure during the preimplantation period affects blastocyst lineages and offspring development

Burkuš

Kačmarová

Kubandová

et al. 2015

Journal of Reproduction and Development

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We found retardation of preimplantation embryo growth after exposure to maternal restraint stress during the preimplantation period in our previous study. In the present study, we evaluated the impact of preimplantation maternal restraint stress on the distribution of inner cell mass (ICM) and trophectoderm (TE) cells in mouse blastocysts, and its possible effect on physiological development of offspring. We exposed spontaneously ovulating female mice to restraint stress for 30 min three times a day during the preimplantation period, and this treatment caused a significant increase in blood serum corticosterone concentration. Microscopic evaluation of embryos showed that restraint stress significantly decreased cell counts per blastocyst. Comparing the effect of restraint stress on the two blastocyst cell lineages, we found that the reduction in TE cells was more substantial than the reduction in ICM cells, which resulted in an increased ICM/TE ratio in blastocysts isolated from stressed dams compared with controls. Restraint stress reduced the number of implantation sites in uteri, significantly delayed eye opening in delivered mice, and altered their behavior in terms of two parameters (scratching on the base of an open field test apparatus, time spent in central zone) as well. Moreover, prenatally stressed offspring had significantly lower body weights and in 5-week old females delivered from stressed dams, fat deposits were significantly lower. Our results indicate that exposure to stress during very early pregnancy can have a negative impact on embryonic development with consequences reaching into postnatal life.

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“…If the fetus expresses vasopressin receptors (or adrenergic receptors in the case of epinephrine), this can also lead to physiologic changes in the fetus. No safe drug levels of vasopressin or epinephrine have been established in the first trimester and early second trimester, but animal embryos have been documented to express vasopressin receptors at as early as twelve conceptional days, and adrenergic receptors before implantation (Chen, Schreiber, and Brinton 2000; Čikoš et al 2013).…”

Section: Review Of the Literature And Ethical Considerations: Interstmentioning

confidence: 99%

The Ethics of Interstitial and Cesarean Scar Ectopic Pregnancies

Buskmiller

2018

Linacre Q

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Catholic bioethicists have extensively addressed extrauterine tubal pregnancies, which represent the great majority of ectopic pregnancies. However, additional management options have been developed for the other 7-10 percent of ectopic pregnancies. Using two cases of interstitial pregnancy and two cases of cesarean scar pregnancy (CSP) seen at a Catholic tertiary care center, this article discusses options including expectant management, systemic methotrexate, intragestational methotrexate, intragestational potassium chloride, uterine artery embolization, dilation and curettage (D&C), vasopressin use, cornuostomy, cornual wedge resection, CSP evacuation, CSP scar excision, CSP salvage, and hysterectomy. Cornual wedge resection, vasopressin use, and CSP scar excision are morally acceptable; less clearly licit are aspiration of gestational sac contents, cornuostomy, gestational excision for CSPs, and methotrexate. Certainly illicit are any techniques leading to direct abortion such as D&Cs on live embryos or fetuses, double-balloon catheter placement, and use of potassium chloride. Summary: An ectopic pregnancy is any pregnancy outside the uterus. These are dangerous because the pregnancy can burst out of its abnormal location and cause life-threatening internal bleeding. Most are in the part of the fallopian tube outside the uterus, but there are other types, including interstitial pregnancies (located in the part of the tube tunneling through the uterine wall) and cesarean scar pregnancies (buried in the uterine scar where the cut for a C-section was made). This article lists the ways that physicians prevent women from dying from interstitial and cesarean scar pregnancies and proposes which treatments are morally acceptable.

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Expression of Adrenergic Receptors in Bovine and Rabbit Oocytes and Preimplantation Embryos

Cited by 15 publications

References 37 publications

Identification of Functional Food Factors as β<sub>2</sub>-Adrenergic Receptor Agonists and Their Potential Roles in Skeletal Muscle

Identification of Functional Food Factors as β<sub>2</sub>-Adrenergic Receptor Agonists and Their Potential Roles in Skeletal Muscle

Stress exposure during the preimplantation period affects blastocyst lineages and offspring development

The Ethics of Interstitial and Cesarean Scar Ectopic Pregnancies

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