Expression of AMPA, kainate, and NMDA receptor subunits in cochlear and vestibular ganglia

Niedzielski, A.S.; Wenthold, RJ

doi:10.1523/jneurosci.15-03-02338.1995

Cited by 168 publications

(109 citation statements)

References 47 publications

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“…The cochlear and vestibular ganglia of the rat exhibit high levels of NR1, moderate levels of NR2B and NR2D, and lower levels of NR2A and NR2C (Niedzielski and Wenthold, 1995). Furthermore, NR1 staining of neuronal somata in some regions of the gerbil cochlear nucleus increases from P7 to P28 (Joelson and Schwartz, 1998).…”

Section: Glutamate Receptors In Mammalian and Avian Auditory Developmentmentioning

confidence: 98%

Development of NMDA R1 expression in chicken auditory brainstem

Tang

Carr

2004

Hearing Research

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N-methyl-D-aspartate (NMDA) receptor subunit-specific probes were used to characterize developmental changes in the distribution of excitatory amino acid receptors in the chicken's auditory brainstem nuclei. Although NR1 subunit expression does not change greatly during the development of the cochlear nuclei in the chicken Hear. , there are significant developmental changes in NR2 subunit expression. We used in situ hybridization against NR1, NR2A, NR2B, NR2C, and NR2D to compare NR1 and NR2 expression during development. All five NMDA subunits were expressed in the auditory brainstem before embryonic day (E) 10, when electrical activity and synaptic responses appear in the nucleus magnocellularis (NM) and the nucleus laminaris (NL). At this time, the dominant form of the receptor appeared to contain NR1 and NR2B. NR2A appeared to replace NR2B by E14, a time that coincides with synaptic refinement and evoked auditory responses. NR2C did not change greatly during auditory development, whereas NR2D increased from E10 and remained at fairly high levels into adulthood. Thus changes in NMDA NR2 receptor subunits may contribute to the development of auditory brainstem responses in the chick. Indexing termscochlear nucleus; magnocellularis; laminaris; angularis; tonotopic gradient Physiological studies have shown that the auditory system is characterized by fast, accurate encoding of auditory information (Warchol and Dallos, 1990; Zhang and Trussell, 1994a,b;Fukui and Ohmori, 2003). We have therefore investigated the distribution of subtypes of Nmethyl-D-aspartate (NMDA) receptors (NMDAR) in the brainstem auditory nuclei of the chicken to determine whether changes in receptors may reflect the development of specializations for temporal processing. NMDAR can contain two classes of subunits, NR1 and NR2 (A-D). The third class of subunits (NR3), containing NR3A and NR3B, is thought to act as modulators of the NMDAR (Ciabarra et al., 1995;Nishi et al., 2001; for review see Cull-Candy, 2001). A functional NMDAR appears to consist of two NR1 subunits and two or three NR2 subunits. NR1 is the fundamental subunit necessary for the NMDAR complex, and its expression is spatiotemporally ubiquitous compared with that of NR2 subunits in vertebrate brain (Watanabe et al., 1992;Wada et al., 2004).Changes in NMDAR composition characterize the development of forebrain circuits. NR2B has been shown to be necessary for synapse formation and plasticity (Tang et al., 1999;Slutsky et al., 2004), whereas NR2A expression is associated with synaptic maturation (Fu et al., 2005). NMDAR made up of NR2B and NR1 subunits are the dominant form during NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript the "critical period" window for plasticity in mouse visual cortex (Quinlan et al., 1999a) and barrel cortex ) and in vocal learning regions of songbirds (Basham et al., 1999;Singh et al., 2000;Ding and Perkel, 2004). Near the end of the critical period, there is a gradual increase in the contribution of NR2A subunits i...

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Section: Glutamate Receptors In Mammalian and Avian Auditory Developmentmentioning

confidence: 98%

Development of NMDA R1 expression in chicken auditory brainstem

Tang

Carr

2004

Hearing Research

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“…8, which is published as supporting information on the PNAS web site). We also tested expression of three glutamate receptors (GluR2-4), because these AMPA receptors are expressed at high levels in type I spiral ganglia neurons as well as dorsal root ganglia neurons (23,24). Our RT-PCR analysis showed that GluR2 was not detectable in MSCs regardless of their differentiation status (data not shown).…”

Section: Bone Mscs Constitutively Express Neural Stem Cell Markers Andmentioning

confidence: 99%

Sonic hedgehog and retinoic acid synergistically promote sensory fate specification from bone marrow-derived pluripotent stem cells

Kondo

Johnson

Yöder

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

164

118

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Recent studies demonstrated that stromal cells isolated from adult bone marrow have the competence of differentiating into neuronal cells in vitro and in vivo. However, the capacity of marrow stromal cells or mesenchymal stem cells (MSCs) to differentiate into diverse neuronal cell populations and the identity of molecular factors that confer marrow stromal cells with the competence of a neuronal subtype have yet to be elucidated. Here, we show that Sonic hedgehog (Shh) and retinoic acid (RA), signaling molecules secreted from tissues in the vicinity of peripheral sensory ganglia during embryogenesis, exert synergistic effects on neural-competent MSCs to express a comprehensive set of glutamatergic sensory neuron markers. Application of Shh or RA alone had little or no effect on the expression of these neuronal subtype markers. In addition, incubation of MSCs with embryonic hindbrain͞somite͞ otocyst conditioned medium or prenatal cochlea explants promoted up-regulation of additional sensory neuron markers and process outgrowth. These results identify Shh and RA as sensory competence factors for adult pluripotent cells and establish the importance of interactions between adult pluripotent cells and the host microenvironment in neuronal subtype specification.

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“…So we cannot make a reasonable prediction of the composition of the subunits in the kainate receptors in the cochlea. A previous RT-PCR and in situ hybridization analysis of kainate receptor subunits in rat cochlear ganglion by Niedzielski and Wenthold (1995) observed all but the GluK3 subunit. It is possible that some of the antibodies we used may have reacted with GluK subunit other than those to which they were created, so that we may have erroneously attributed one or more subunits to the hair cell synapse, when in fact its presence in our mRNA analysis may have been associated with role in the axonal terminal in the cochlear nucleus.…”

Section: Fig 6 Examples Of Synaptic Plaques Showing Non-overlappingmentioning

confidence: 85%

“…However, information on the presence, distribution, and role of kainate receptors in the cochlea is sparse. Kainate receptor subunits (GluK1, 2, 4, and 5) are known to be present in spiral ganglion cell bodies by in situ hybridization (Niedzielski and Wenthold 1995), but it is not known whether the receptors, once generated, are distributed at the hair cell afferent synapse, on the presynaptic terminals in the cochlear nucleus, or in both locations. Immunohistochemical analysis of ionotropic receptors in the cochlea has always been challenging.…”

Section: Introductionmentioning

confidence: 99%

Cochlear Kainate Receptors

Peppi

Landa

Sewell

2012

JARO

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Synaptic transmission between the cochlear hair cell and its afferent fiber is mediated by glutamate receptors. While kainate receptors are known to be present in the spiral ganglion, little is known of their distribution or functional role. We have detected all five kainate receptor subunits in the mouse cochlea with quantitative RT-PCR and with immunohistochemistry. We observed kainate receptors on afferent terminals co-localized with α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors at the afferent synapse. Individual terminals innervating a single hair cell varied in their ratios of AMPA to kainate receptor immunoreactivity. Infusion of the mouse cochlea via the scala tympani with UBP296, a recently developed antagonist with high specificity for the GluK1 kainate receptor (compared to the AMPA receptor), reduced the compound action potential and elevated auditory neural thresholds without affecting the distortion product otoacoustic emission thresholds. Thus, the pharmacological evidence suggests that kainate receptors may contribute to the response to transmitter released from the hair cell during acoustic stimulation. It is plausible that afferent transmission at this synapse is mediated by a mix of AMPA and kainate receptors.

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Expression of AMPA, kainate, and NMDA receptor subunits in cochlear and vestibular ganglia

Cited by 168 publications

References 47 publications

Development of NMDA R1 expression in chicken auditory brainstem

Development of NMDA R1 expression in chicken auditory brainstem

Sonic hedgehog and retinoic acid synergistically promote sensory fate specification from bone marrow-derived pluripotent stem cells

Cochlear Kainate Receptors

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