Expression of an Atriplex nummularia gene encoding a protein homologous to the bacterial molecular chaperone DnaJ.

Zhu, Jian‐Kang; Shi, Jun; Bressan, Ray A.; Hasegawa, Paul M.

doi:10.1105/tpc.5.3.341

Cited by 51 publications

(19 citation statements)

References 26 publications

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“…The upregulation of dnaJ expression also correlated with the upregulation of the flavonoid biosynthesis genes and flavonoid accumulation. DnaJ is also involved in salt stress resistance and known to interact with Hsp70 in the heat shock resistance process (Zhu et al, 1993). Since dnaJ expression was also found to be involved in regulation of saline tolerance, it is reasonable to test whether the higher expression of dnaJ will also result in increased salt stress tolerance by the plants.…”

Section: Discussionmentioning

confidence: 99%

Systemic Responses of Barley to the 3-hydroxy-decanoyl-homoserine Lactone Producing Plant Beneficial Endophyte Acidovorax radicis N35

Han

Trost

et al. 2016

Front. Plant Sci.

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Quorum sensing auto-inducers of the N-acyl homoserine lactone (AHL) type produced by Gram-negative bacteria have different effects on plants including stimulation on root growth and/or priming or acquirement of systemic resistance in plants. In this communication the influence of AHL production of the plant growth promoting endophytic rhizosphere bacterium Acidovorax radicis N35 on barley seedlings was investigated. A. radicis N35 produces 3-hydroxy-C10-homoserine lactone (3-OH-C10-HSL) as the major AHL compound. To study the influence of this QS autoinducer on the interaction with barley, the araI-biosynthesis gene was deleted. The comparison of inoculation effects of the A. radicis N35 wild type and the araI mutant resulted in remarkable differences. While the N35 wild type colonized plant roots effectively in microcolonies, the araI mutant occurred at the root surface as single cells. Furthermore, in a mixed inoculum the wild type was much more prevalent in colonization than the araI mutant documenting that the araI mutation affected root colonization. Nevertheless, a significant plant growth promoting effect could be shown after inoculation of barley with the wild type and the araI mutant in soil after 2 months cultivation. While A. radicis N35 wild type showed only a very weak induction of early defense responses in plant RNA expression analysis, the araI mutant caused increased expression of flavonoid biosynthesis genes. This was corroborated by the accumulation of several flavonoid compounds such as saponarin and lutonarin in leaves of root inoculated barley seedlings. Thus, although the exact role of the flavonoids in this plant response is not clear yet, it can be concluded, that the synthesis of AHLs by A. radicis has implications on the perception by the host plant barley and thereby contributes to the establishment and function of the bacteria-plant interaction.

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Section: Discussionmentioning

confidence: 99%

Systemic Responses of Barley to the 3-hydroxy-decanoyl-homoserine Lactone Producing Plant Beneficial Endophyte Acidovorax radicis N35

Han

Trost

et al. 2016

Front. Plant Sci.

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“…Clone A12B15-6 was differentially expressed only during water deficit in the two drought tolerant genotypes (Siokra L-23 and T-1521; table 1, figure 2). Most of the HSP seem to function as molecular chaperones that assist in protein folding and prevention of protein denaturation (Zhu et al, 1993). Water deficit, osmotic stress and cold stress are some of the abiotic stresses that aggravate protein aggregation and denaturation, making production of HS proteins more necessary.…”

Section: Discussionmentioning

confidence: 99%

“…The ability to respond to mild temperature shock by the synthesis of HSP within few hours of the shock is a general response and has been observed in microbes and animals as well as in plants (Harborne, 1997). Most of the HSP probably function as molecular chaperones that assist in protein folding and prevent protein denaturation (Zhu et al, 1993). Some HSP are normally produced by the cell, while during stress more HSP are necessary because protein aggregation and denaturation are increased (Cooper, 1997).…”

Section: Introductionmentioning

confidence: 99%

Expression of heat shock protein and trehalose-6-phosphate synthase homologues induced during water deficit in cotton

Nepomuceno

Oosterhuis

Stewart

et al. 2002

Braz. J. Plant Physiol.

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Tolerance to drought in plants is not a simple trait, but a complex of mechanisms working in combination to avoid or to resist water deficit. Genotypes that differ in tolerance to water deficit may show qualitative and quantitative differences in gene expression when submitted to drought periods. Four cotton (Gossypium hirsutum L.) genotypes (Siokra L-23, Stoneville 506, CS 50 and T-1521) with contrasting responses to water deficit stress were studied using the Differential Display (DD) technique to identify and isolate genes which may differ among them. Fifty-two cDNA fragments differentially expressed during water deficit were isolated, cloned and sequenced. Search of gene bank databases showed that two cDNA clones, A12B15-6 and A12B13-1, have high homology with a heat shock protein that binds to calmodulin found in Nicotiana tabacum (2.9e-32 P(N)) and with an Arabidopsis thaliana trehalose-6-phosphate synthase enzyme (9.0e-37 P(N)), respectively. One of the presumed functions of heat shock proteins is related to prevention of protein denaturation during cellular dehydration. Trehalose-6-phosphate synthase is involved in the production of trehalose, a disaccharide known to osmotically protect cell membranes during dehydration. The HSP homologue was found to be differentially expressed during the drought period in two drought tolerant genotypes but not in drought-sensitive genotypes. The trehalose-6-phosphate synthase homologue was also up-regulated during water deficit stress, however, all four genotypes were induced to express this homologue. Ribonuclease protection assays confirmed these results. This is an important finding since there are only few reports of trehalose presence in higher plants and none in cotton.

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“…Thus, DnaJ-like proteins, in association with members of the Hsp70 family, are critically involved in the folding of proteins, the assembly of protein complexes, and the cellular response to heat shock. 156 Heat shock has been shown to cause the accumulation of ANJ1 and Hsp70 transcripts in A. nummularia cells 155 and to increase the amount of prenylated DnaJ protein associated with glyoxosomal membranes in cucumber seedlings, 159 consistent with a role for DnaJ-like proteins in the heat shock response of plant cells. 157,158 ANJ1 was a substrate for in vitro isoprenylation (farnesylation and, to some extent, geranylgeranylation) when extracts from Atriplex nummularia or Saccharomyces cerevisiae were used as a source of prenyl:protein transferase activity.…”

Section: Protein Isoprenylation In Plantsmentioning

confidence: 86%

“…155,156 The Atriplex nummularia ANJ1 protein is a 47-kDa polypeptide with 35% amino acid sequence identity to the Escherichia coli DnaJ protein. 155,156 The Atriplex nummularia ANJ1 protein is a 47-kDa polypeptide with 35% amino acid sequence identity to the Escherichia coli DnaJ protein.…”

Section: Protein Isoprenylation In Plantsmentioning

confidence: 99%

Protein Isoprenylation in Plants

Randall¹,

Crowell²

1999

Critical Reviews in Biochemistry and Molecular Biology

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Expression of an Atriplex nummularia gene encoding a protein homologous to the bacterial molecular chaperone DnaJ.

Cited by 51 publications

References 26 publications

Systemic Responses of Barley to the 3-hydroxy-decanoyl-homoserine Lactone Producing Plant Beneficial Endophyte Acidovorax radicis N35

Systemic Responses of Barley to the 3-hydroxy-decanoyl-homoserine Lactone Producing Plant Beneficial Endophyte Acidovorax radicis N35

Expression of heat shock protein and trehalose-6-phosphate synthase homologues induced during water deficit in cotton

Protein Isoprenylation in Plants

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