Expression of an L1-Related Cell Adhesion Molecule on Developing CNS Fiber Tracts in Zebrafish and Its Functional Contribution to Axon Fasciculation

Weiland, Ulrich; Ott, Heiko; Bastmeyer, Martin; Schaden, Herbert; Giordano, Suzanne; Stuermer, Claudia A. O.

doi:10.1006/mcne.1997.0603

Cited by 26 publications

(28 citation statements)

References 49 publications

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“…Images were edited with Photoshop 6.0 (Adobe). Zebrafish embryos were processed for whole-mount immunohistochemistry as previously described (Marx et al, 2001;Weiland et al, 1997). Embryos were labeled with zebrafish pAb Tag-1 (axons, Lang et al, 2001), anti-␣-actinin (Biotrend), antiphospho-Tyrosin (myotome boundaries, Santa Cruz Biotechnologiy), and phalloidin-Alexa 488 (actin, Invitrogen).…”

Section: Whole Mounts In Situ Hybridization and Immunostainingmentioning

confidence: 99%

Molecular evolution and expression of zebrafish St8SiaIII, an alpha‐2,8‐sialyltransferase involved in myotome development

Bentrop

Marx

Schattschneider

et al. 2008

Developmental Dynamics

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Enzymes of the St8Sia family, a subgroup of the glycosyltransferases, mediate the transfer of sialic acid to glycoproteins or glycolipids. Here, we describe the cloning of the zebrafish St8SiaIII gene and study its developmental activity. A conserved synteny relationship among vertebrate chromosome regions containing St8SiaIII loci underscores an ancient duplication of this gene in the teleost fish lineage and a specific secondary loss of one paralog in the zebrafish. The single zebrafish St8SiaIII enzyme, which is expected to function as an oligosialyltransferase, lacks maternal activity, is weakly expressed during nervous system development, and shows a highly dynamic expression pattern in somites and somitederived structures. Morpholino knock-down of St8SiaIII leads to anomalous somite morphologies, including defects in segment boundary formation and myotendious-junction integrity. These phenotypes hint for a basic activity of zebrafish St8SiaIII during segmentation and somite formation, providing novel evidence for a non-neuronal function of sialyltransferases during vertebrate development. Developmental Dynamics 237:808 -818, 2008.

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Section: Whole Mounts In Situ Hybridization and Immunostainingmentioning

confidence: 99%

Molecular evolution and expression of zebrafish St8SiaIII, an alpha‐2,8‐sialyltransferase involved in myotome development

Bentrop

Marx

Schattschneider

et al. 2008

Developmental Dynamics

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“…A polyclonal serum against c-Jun, which recognizes all three Jun proteins (c-Jun, Jun B, and Jun D) (Kovary and Bravo, 1991), was used at 1:8000 for immunohistochemistry and 1:10,000 for Western blot analysis (antibody 636/3; kindly provided by Thomas Herdegen), and the resulting staining is referred to as Jun-like immunoreactivity (Jun-IR). Also used were E17, a mAb against E587 antigen (E587-Ag), an L1-like cell-adhesion molecule in goldfish (Weiland et al, 1997); N518, a mAb against neurolin, the goldfish homolog of DM-GR ASP (Bastmeyer et al, 1995;Leppert et al, 1999); R643 mAb against reggie-2 from goldfish (Schulte et al, 1997;Lang et al, 1998); and a polyclonal serum against goldfish reggie-2 (Lang et al, 1998). Secondary antibodies included the following: Indocarbocyanine (C y3)-conjugated donkey anti-mouse IgG at 1:1000 (Jackson ImmunoResearch, West Grove, PA) was used to recognize all of the monoclonal antibodies used.…”

Section: Methodsmentioning

confidence: 99%

A Purine-Sensitive Pathway Regulates Multiple Genes Involved in Axon Regeneration in Goldfish Retinal Ganglion Cells

et al. 2000

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In lower vertebrates, retinal ganglion cells (RGCs) can regenerate their axons and reestablish functional connections after optic nerve injury. We show here that in goldfish RGCs, the effects of several trophic factors converge on a purine-sensitive signaling mechanism that controls axonal outgrowth and the expression of multiple growth-associated proteins. In culture, goldfish RGCs regenerate their axons in response to two molecules secreted by optic nerve glia, axogenesis factor-1 (AF-1) and AF-2, along with ciliary neurotrophic factor. The purine analog 6-thioguanine (6-TG) blocked outgrowth induced by each of these factors. Previous studies in PC12 cells have shown that the effects of 6-TG on neurite outgrowth may be mediated via inhibition of a 47 kDa protein kinase. Growth factor-induced axogenesis in RGCs was accompanied by many of the molecular changes that characterize regenerative growth in vivo, e.g., increased expression of GAP-43 and certain cell surface glycoproteins. 6-TG inhibited all of these changes but not those associated with axotomy per se, e.g., induction of jun family transcription factors, nor did it affect cell survival. Additional studies using RGCs from transgenic zebrafish showed that expression of T␣-1 tubulin is likewise stimulated by AF-1 and blocked by 6-TG. The purine nucleoside inosine had effects opposite to those of 6-TG. Inosine stimulated outgrowth and the characteristic pattern of molecular changes in RGCs and competitively reversed the inhibitory effects of 6-TG. We conclude that axon regeneration and the underlying program of gene expression in goldfish RGCs are mediated via a common, purine-sensitive pathway.

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“…One member of the CAM family, L1, also plays a role in axonal fasciculation (Stallcup and Beasley, 1985;Lemmon and McLoon, 1986;Weiland et al, 1997). It is required by retinal axons in the tectum, where their pattern is disorganized in the L1-null neonate (Demyanenko and Maness, 2003).…”

Section: Tag-1 Contributes To Rgc Axon Fasciculation In the Optic Nervementioning

confidence: 99%

Structural Requirement of TAG-1 for Retinal Ganglion Cell Axons and Myelin in the Mouse Optic Nerve

Chatzopoulou¹,

Miguez²,

Savvaki³

et al. 2008

J. Neurosci.

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White matter axons organize into fascicles that grow over long distances and traverse very diverse environments. The molecular mechanisms preserving this structure of white matter axonal tracts are not well known. Here, we used the optic nerve as a model and investigated the role of TAG-1, a cell adhesion molecule expressed by retinal axons. TAG-1 was first expressed in the embryonic retinal ganglion cells (RGCs) and later in the postnatal myelin-forming cells in the optic nerve. We describe the consequences of genetic loss of Tag-1 on the developing and adult retinogeniculate tract. Tag-1-null embryos display anomalies in the caliber of RGC axons, associated with an abnormal organization of the astroglial network in the optic nerve. The contralateral projections in the lateral geniculate nucleus are expanded postnatally. In the adult, Tag-1-null mice show a loss of RGC axons, with persistent abnormalities of axonal caliber and additional cytoskeleton and myelination defects. Therefore, TAG-1 is an essential regulator of the structure of RGC axons and their surrounding glial cells in the optic nerve.

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Expression of an L1-Related Cell Adhesion Molecule on Developing CNS Fiber Tracts in Zebrafish and Its Functional Contribution to Axon Fasciculation

Cited by 26 publications

References 49 publications

Molecular evolution and expression of zebrafish St8SiaIII, an alpha‐2,8‐sialyltransferase involved in myotome development

Molecular evolution and expression of zebrafish St8SiaIII, an alpha‐2,8‐sialyltransferase involved in myotome development

A Purine-Sensitive Pathway Regulates Multiple Genes Involved in Axon Regeneration in Goldfish Retinal Ganglion Cells

Structural Requirement of TAG-1 for Retinal Ganglion Cell Axons and Myelin in the Mouse Optic Nerve

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